scholarly journals Comparison of the BD Phoenix System with the Cefoxitin Disk Diffusion Test for Detection of Methicillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci

2009 ◽  
Vol 47 (7) ◽  
pp. 2288-2291 ◽  
Author(s):  
A. Mencacci ◽  
I. Montecarlo ◽  
F. Gonfia ◽  
A. Moretti ◽  
A. Cardaccia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Test ◽  
Cefoxitin Disk ◽  
Cefoxitin Disk Diffusion

scholarly journals Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus

1999 ◽  
Vol 37 (5) ◽  
pp. 1591-1594 ◽  
Author(s):  
Matthias Cavassini ◽  
Aline Wenger ◽  
Katia Jaton ◽  
Dominique S. Blanc ◽  
Jacques Bille
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Screening Test ◽  
Rapid Detection ◽  
Methicillin Resistance ◽  
Screen Test ◽  
Latex Agglutination ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test

The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of themecA gene for the detection of methicillin resistance inStaphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) and 80 methicillin-resistant S. aureus (MRSA) isolates, defined by the absence or presence of the mecA gene, respectively, were tested. The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivities of 100, 61.3, and 82.5% and specificities of 99.2, 96.7, and 98.3%, respectively. We conclude that the MRSA-Screen is a very accurate, reliable, and fast test (15 min) for differentiation of MRSA from MSSA colonies on agar plates.

scholarly journals Evaluation of cefoxitin disk diffusion breakpoint for detection of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs

2012 ◽  
Vol 24 (5) ◽  
pp. 964-967 ◽  
Author(s):  
David A. Bemis ◽  
Rebekah D. Jones ◽  
Ricardo Videla ◽  
Stephen A. Kania
Keyword(s):  
Methicillin Resistance ◽  
Screening Method ◽  
Inhibition Zone ◽  
Disk Diffusion ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcus Pseudintermedius ◽  
Growth Inhibition Zone ◽  
Disk Method ◽  
Cefoxitin Disk ◽  
Cefoxitin Disk Diffusion

Cefoxitin disk diffusion susceptibility testing is a recommended screening method for the detection of methicillin resistance in human isolates of Staphylococcus aureus and coagulase-negative staphylococci. A retrospective analysis of 1,146 clinical isolates of Staphylococcus pseudintermedius from dogs was conducted to determine if screening by the cefoxitin disk method can be similarly useful with S. pseudintermedius. The distribution of cefoxitin growth inhibition zone diameters within this collection was bimodal and correlated well with the results of methicillin resistance gene ( mecA) detection by polymerase chain reaction. Of the isolates, 5% had discordant results and, when retested, 84% of these were in agreement. While a greater diversity of isolates and interlaboratory comparisons must be tested, the current study suggests that an epidemiological breakpoint (of approximately ≤30 mm = resistant; ≥31 = susceptible) can be established to predict methicillin resistance in S. pseudintermedius.

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