Evaluation of a cefoxitin disk diffusion test for the detection of mecA-positive methicillin-resistant Staphylococcus saprophyticus

2006 ◽  
Vol 27 (6) ◽  
pp. 500-504 ◽  
Author(s):  
M HIGASHIDE ◽  
M KURODA ◽  
S OHKAWA ◽  
T OHTA
Keyword(s):  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Methicillin Resistant ◽  
Staphylococcus Saprophyticus ◽  
Cefoxitin Disk ◽  
Cefoxitin Disk Diffusion

scholarly journals Comparison of Different Phenotypic Approaches To Screen and Detect mecC-Harboring Methicillin-Resistant Staphylococcus aureus

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
André Kriegeskorte ◽  
Evgeny A. Idelevich ◽  
Andreas Schlattmann ◽  
Franziska Layer ◽  
Birgit Strommenger ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Disk Diffusion Test ◽  
Methicillin Resistant ◽  
Content Type ◽  
Vitek 2 ◽  
Cefoxitin Disk ◽  
Cefoxitin Disk Diffusion

ABSTRACT Similar to mecA, mecC confers resistance against beta-lactams, leading to the phenotype of methicillin-resistant Staphylococcus aureus (MRSA). However, mecC-harboring MRSA strains pose special difficulties in their detection. The aim of this study was to assess and compare different phenotypic systems for screening, identification, and susceptibility testing of mecC-positive MRSA isolates. A well-characterized collection of mecC-positive S. aureus isolates (n = 111) was used for evaluation. Routinely used approaches were studied to determine their suitability to correctly identify mecC-harboring MRSA, including three (semi)automated antimicrobial susceptibility testing (AST) systems and five selective chromogenic agar plates. Additionally, a cefoxitin disk diffusion test and an oxacillin broth microdilution assay were examined. All mecC-harboring MRSA isolates were able to grow on all chromogenic MRSA screening plates tested. Detection of these isolates in AST systems based on cefoxitin and/or oxacillin testing yielded overall positive agreements with the mecC genotype of 97.3% (MicroScan WalkAway; Siemens), 91.9% (Vitek 2; bioMérieux), and 64.9% (Phoenix, BD). The phenotypic resistance pattern most frequently observed by AST devices was “cefoxitin resistance/oxacillin susceptibility,” ranging from 54.1% (Phoenix) and 83.8% (Vitek 2) to 92.8% (WalkAway). The cefoxitin disk diffusion and oxacillin broth microdilution assays categorized 100% and 61.3% of isolates to be MRSA, respectively. The chromogenic media tested confirmed their suitability to reliably screen for mecC-harboring MRSA. The AST systems showed false-negative results with varying numbers, misidentifying mecC-harboring MRSA as methicillin-susceptible S. aureus. This study underlines cefoxitin's status as the superior surrogate mecC-positive MRSA marker.

scholarly journals Ceftaroline resistance in Staphylococcus pseudintermedius gene mecA carriers

2018 ◽  
Vol 38 (12) ◽  
pp. 2233-2236
Author(s):  
Carolina B. Scherer ◽  
Larissa S. Botoni ◽  
Antônio U. Carvalho ◽  
Kelly M. Keller ◽  
Adriane P. Costa-Val
Keyword(s):  
Methicillin Resistance ◽  
Meca Gene ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Staphylococcus Pseudintermedius ◽  
Ceftaroline Fosamil ◽  
Disk Diffusion Test ◽  
Methicillin Resistant ◽  
Resistant Strains ◽  
Current Report

ABSTRACT: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) being a constant concern, ceftaroline fosamil has been recently approved as a new cephalosporin, active against MRSA, for use in humans; only rare cases of resistance have been reported till date. There is no report of resistance to ceftaroline in Staphylococcus pseudintermedius, which is the main bacterium causing dermatitis and otitis in dogs. To evaluate staphylococcal resistance to ceftaroline, 35 isolates of methicillin-resistant S. pseudintermedius (MRSP), carrying the mecA gene, from 26 dogs with folliculitis and nine dogs with external otitis, underwent disk diffusion test with cefoxitin, oxacillin, and ceftaroline. Tests with cefoxitin and oxacillin showed > 90% sensitivity in methicillin resistance detection. In the disk diffusion test, 97.14% (34/35) were resistant to cefoxitin, 94.29% (33/35) to oxacillin, and 31.43% (11/35) to ceftaroline. Of the ceftaroline-resistant strains, 27.27% (3/11) were obtained from the ears of dogs while the rest (8/11) were from the skin. The current report is the first description of MRSP resistance to ceftaroline.

Incidence and antibiotic susceptibility of methicillin resistant Staphylococcus aureus in pus samples of patients of Bastar region, Central India

2012 ◽  
Vol 2 (5) ◽  
pp. 204-208
Author(s):  
Mohammad Fareed Khan ◽  
Arvind Neral ◽  
Vikas Chandra Yadav ◽  
Farah Aziz Khan ◽  
Sarfaraz Ahmed
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Central India ◽  
Empirical Therapy ◽  
Disk Diffusion ◽  
Antibiotic Prescribing ◽  
Care Hospital ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Methicillin Resistant

Increasing prevalence of methicillin resistant S. aureus (MRSA) and resistanceto multiple antibiotic classes is a global issue. Regional surveillance of antibiotic susceptibility of the organism is a necessary step to overcome the issues of antimicrobial resistance and treatment failure in MRSA infections. The study was conducted to find the pattern of antibiotic susceptibility in MRSA isolated from the pus samples of patients attending a tertiary care hospital in Bastar tribal region in Central India. The study was performed on 215 MRSA isolates cultured from pus samples of patients over a period of two years and five months. In the methicillin resistant organisms selected by oxacillin screen agar test and cefoxitin disk diffusion test, antibiotic susceptibility was determined by Kirby Baur disk diffusion test with CLSI guide lines. Of the total S. aureus isolates, the incidence of MRSA was 34.1% of which 82.8% were resistant to co‐trimoxazole, 77.2% to tetracycline, 68.8% to gentamycin, 66% to erythromycin, 64.2% to ciprofloxacin, 1.4% to vancomycin, and 0.9% to linezolid. All these isolates were resistant to the ß‐lactam antibiotics tested. Emergence of linezolid resistance and relatively higher vancomycin resistance in the MRSA isolates is a worrisome finding of this study. The antibiotic prescribing must rely on both initial empirical therapy and microbiological antibiotic susceptibility result.

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