scholarly journals Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Pulsed-Field Gel Electrophoresis Typing of Acinetobacter baumannii in China

2013 ◽  
Vol 51 (4) ◽  
pp. 1263-1268 ◽  
Author(s):  
Y. Hu ◽  
B. Li ◽  
D. Jin ◽  
Z. Cui ◽  
X. Tao ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Tandem Repeat ◽  
Gel Electrophoresis ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Multiple-Locus Variable-Number Tandem Repeat Analysis ofVibrio choleraein Comparison with Pulsed Field Gel Electrophoresis and Virulotyping

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Cindy Shuan Ju Teh ◽  
Kek Heng Chua ◽  
Kwai Lin Thong
Keyword(s):  
Tandem Repeat ◽  
Gel Electrophoresis ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Multiple Locus ◽  
Repeat Analysis ◽  
Geographical Regions ◽  
Different Sources

Molecular analysis of MalaysianVibrio choleraewas carried out using a multiple-locus variable-number tandem repeat analysis (MLVA) assay based on 7 loci ofV. cholerae. The discriminatory ability of the assay was compared with pulsed-field gel electrophoresis (PFGE) using 43 MalaysianV. choleraeisolated from various sources. In addition, the virulotypes of the strains were determined. Based on MLVA, 38 allelic profiles were obtained (F=0.63) while PFGE generated 35 pulsotypes (F=0.71). Simpson's index of diversity for different VNTR loci ranged from 0.59 to 0.92. The combined loci increased the discriminatory index to 0.99 which was comparable with PFGE (D=0.99). Most of the environmental non-O1/non-O139 strains harboredrtxA,rstR,toxR, andhlyA only, and the virulotype of this serogroup was significantly different (P<.01) from clinical/environmental O1 and environmental O139 strains. In conclusion, the MLVA assay developed in this study was a useful genotyping tool with comparable discriminatory power with PFGE. In addition, the combination of the two approaches can further distinguish the strains from different sources and geographical regions of isolation.

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