Ciprofloxacin Resistance in Campylobacter jejuniIsolates: Detection of gyrA Resistance Mutations by Mismatch Amplification Mutation Assay PCR and DNA Sequence Analysis
1999 ◽
Vol 37
(10)
◽
pp. 3276-3280
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Keyword(s):
The gyrA quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible Campylobacter jejuni isolates. All isolates resistant to ciprofloxacin had Thr-86-to-Ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. A mismatch amplification mutation assay (MAMA) PCR protocol was developed that detects this gyrA mutation in quinolone-resistant isolates. The MAMA PCR provides a means for routine detection of the gyrA mutation without the need for sequencing the gyrA gene.
2020 ◽
Vol 154
(6)
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pp. 947-960
Keyword(s):
Keyword(s):
1991 ◽
Vol 266
(6)
◽
pp. 3752-3759
1996 ◽
Vol 24
(3)
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pp. 901-904
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2012 ◽
Vol 302
(1)
◽
pp. 4-9
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1986 ◽
Vol 261
(17)
◽
pp. 7952-7957
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Keyword(s):
Keyword(s):