Assessment of Use of the COBAS AMPLICOR System with BACTEC 12B Cultures for Rapid Detection of Frequently Identified Mycobacteria
1999 ◽
Vol 37
(3)
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pp. 782-784
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Keyword(s):
The use of the COBAS AMPLICOR System (Roche Molecular Diagnostics, Basel, Switzerland), the only automated system for PCR testing, was evaluated for a rapid identification of mycobacteria with positive BACTEC 12B cultures. Two hundred ninety-six specimens with a growth index of ≥30 were analyzed for the presence of Mycobacterium tuberculosis complex, Mycobacterium avium, andMycobacterium intracellulare. Compared to traditional methods and provided that samples with PCR inhibition are retested at a 1:10 dilution, the sensitivity and specificity of the COBAS AMPLICOR System with BACTEC 12B cultures were 100 and 98%, respectively. The COBAS AMPLICOR method is rapid and reliable for identifying the most common mycobacteria in cultures.
1991 ◽
Vol 29
(11)
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pp. 2473-2476
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1995 ◽
Vol 9
(5)
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pp. 321-326
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1996 ◽
Vol 40
(7)
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pp. 1748-1750
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2005 ◽
Vol 43
(11)
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pp. 5435-5439
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Keyword(s):
2012 ◽
pp. 171-199
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1989 ◽
Vol 27
(7)
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pp. 1543-1547
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1996 ◽
Vol 2
(3)
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pp. 232-233
1987 ◽
Vol 25
(7)
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pp. 1239-1243
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