scholarly journals Performance of Three Enzyme Immunoassays and Two Direct Fluorescence Assays for Detection of Giardia lamblia in Stool Specimens Preserved in ECOFIX

2000 ◽  
Vol 38 (7) ◽  
pp. 2781-2783 ◽  
Author(s):  
Daniel P. Fedorko ◽  
Esther C. Williams ◽  
Nancy A. Nelson ◽  
Leslie B. Calhoun ◽  
Sizhuang S. Yan
Keyword(s):  
Giardia Lamblia ◽  
Fluorescent Antibody ◽  
The Other ◽  
Specific Enzyme ◽  
Enzyme Immunoassays ◽  
Direct Fluorescent Antibody ◽  
Direct Fluorescence ◽  
Stool Specimens ◽  
The Difference ◽  
Formalin Fixed

ECOFIX is a single-vial stool preservative that is both formalin- and mercury-free. We evaluated the abilities of three commercialGiardia lamblia-specific enzyme immunoassays (EIAs) (ProSpecT Giardia Microplate Assay [Alexon-Trend Inc.], Giardia Test [Techlab], and Premier Giardia lamblia [Meridian Diagnostics, Inc.]) and two commercial direct fluorescent-antibody (FA) assays for G. lamblia(Crypto/Giardia IF Test [Techlab] and Merifluor Cryptosporidium/Giardia [Meridian Diagnostics, Inc.]) to detectG. lamblia in 34 G. lamblia-positive and 44G. lamblia-negative stool specimens (determined by traditional examination for ova and parasites) preserved in ECOFIX compared to their abilities to detect G. lamblia in the same specimens preserved in formalin as the “gold standard” for each assay. Of the 34 formalin-fixed positive specimens, the number detected by each assay was as follows:, Alexon EIA, 34; Meridian EIA, 27; Techlab EIA, 29; Meridian FA assay, 31; and Techlab FA assay, 28. Both FA tests and the Alexon EIA performed well with ECOFIX, but the other two EIAs detected fewer positive specimens (the difference was statistically significant with the Techlab EIA) when ECOFIX was the preservative. Use of G. lamblia cyst antigen from cultured organisms preserved in formalin and ECOFIX demonstrated that the Alexon EIA could detect smaller amounts of antigen in ECOFIX than the other two EIAs could and suggested that cyst antigen is more stable in formalin. We recommend that laboratories use an FA assay or the Alexon EIA if they plan to use ECOFIX as their stool preservative.

scholarly journals Detection ofCryptosporidium molnariOocysts from Fish by Fluorescent-Antibody Staining Assays forCryptosporidiumspp. Affecting Humans

2011 ◽  
Vol 77 (5) ◽  
pp. 1878-1880 ◽  
Author(s):  
Rona Barugahare ◽  
Michelle M. Dennis ◽  
Joy A. Becker ◽  
Jan Šlapeta
Keyword(s):  
Ribosomal Dna ◽  
Fluorescent Antibody ◽  
Small Subunit ◽  
Rapid Diagnosis ◽  
Antibody Staining ◽  
Direct Fluorescent Antibody ◽  
Murray Cod ◽  
Diagnosis Of Infection ◽  
Formalin Fixed ◽  
Fluorescent Antibody Staining

ABSTRACTThree direct fluorescent-antibody staining assay kits for the detection of zoonoticCryptosporidiumspecies were used to detectCryptosporidium molnarifrom Murray cod, and the cryptosporidia were characterized by using small-subunit (SSU) ribosomal DNA (rDNA). To facilitate rapid diagnosis of infection, this study demonstrated that all three kits detected freshC. molnariand two kits detected formalin-fixed oocysts.

A 34-year retrospective study of equine viral abortion in Poland

2014 ◽  
Vol 17 (4) ◽  
pp. 607-612 ◽  
Author(s):  
B.A. Bażanów ◽  
A.B. Frącka ◽  
N.A. Jackulak ◽  
Z.M. Staroniewicz ◽  
S.M. Ploch
Keyword(s):  
Virus Isolation ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The Other ◽  
Indirect Fluorescent Antibody ◽  
Direct Fluorescent Antibody ◽  
Tissue Homogenates ◽  
Vero Cell Lines ◽  
Isolation Test

Abstract The purpose of the present review was a comparison of the abortions caused by EAV and EHV-1 viruses over the 34 years. A total of 452 tissues samples from aborted fetuses (347) or foals (105) stillborn or newborn that died within 72 hours were investigated. The material for the examinations came from different farms located throughout Poland. The tissue homogenates were examined by using virus isolation test in RK-13 and Vero cell lines and the cytopathic agent was confirmed as EHV-1 by the direct fluorescent antibody test or as EAV by the indirect fluorescent antibody test. The study indicated that EAV was isolated (104 cases, 23%) almost as equally often as EHV-1 (116 cases, 25.6%). Both, equid herpesvirus-associated abortion and the abortion induced by EAV were characterized by cyclicity. The percentage of EAV and EHV-1 isolation alternately reduced and increased, but the increase of isolation of one virus was accompanied by the decrease of the other. The domination of one virus over the other occurred in cycles of a few years.

Evaluation of four methods for detection of group B streptococcal colonization

1976 ◽  
Vol 4 (5) ◽  
pp. 429-431
Author(s):  
E O Mason ◽  
P Wong ◽  
F F Barrett
Keyword(s):  
Fluorescent Antibody ◽  
Positive Culture ◽  
The Other ◽  
Antibody Staining ◽  
Direct Fluorescent Antibody ◽  
Group B ◽  
Site Selective ◽  
Sensitive Method ◽  
Fluorescent Antibody Staining

Four methods (streak plate, pour plate, selective broth, and direct fluorescent-antibody staining) were evaluated for their ability to detect group B streptococcal colonization in parturient women and their offspring. When colonization was defined as a positive culture by any method from any site, selective broth was the most sensitive method, detecting 100% of colonized mothers and infants at birth and 48 h of age. This method failed to detect only one colonized individual (infant at 24 h of age). The other three methods detected from 20 to 56% of colonized individuals.

scholarly journals PREVALENCE OF TOXOPLASMOSIS AMONG SHEEP AND GOATS IN BAGHDAD AREA

2021 ◽  
Vol 22 (1) ◽  
pp. 43-49
Author(s):  
Layla Kh.Rifaat ◽  
Suad Z.Jawdat
Keyword(s):  
Toxoplasma Gondii ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Fixation Test ◽  
The Other ◽  
Sheep And Goats ◽  
Combined Use ◽  
Direct Fluorescent Antibody

The complement fixation test (CFT) and the direct fluorescent antibody test were used for detecting anti Toxoplasma gondii antibodies in sera obtained from 143 sheep and 44 goats. Complement fixing antibodies were detected in 38 (26.2%) for sheep sera and 24 (54.5%) of goat sera tested by CFT.  On the other hand, 26(18.2%) of sheep sera were positive by the FAT. The combined use of CFT and FAT allows the differentiation between an acute or latent T.gondii infection.

scholarly journals Comparison of Nine Commercially Available Enzyme-Linked Immunosorbent Assays for Detection of Giardia lamblia in Fecal Specimens

1998 ◽  
Vol 36 (5) ◽  
pp. 1338-1340 ◽  
Author(s):  
William E. Aldeen ◽  
K. Carroll ◽  
A. Robison ◽  
M. Morrison ◽  
D. Hale
Keyword(s):  
Giardia Lamblia ◽  
Polyclonal Antibodies ◽  
Fluorescent Antibody ◽  
Ease Of Use ◽  
Antibody Assay ◽  
Processing Times ◽  
Hands On ◽  
Direct Fluorescent Antibody ◽  
Overall Performance ◽  
Immunosorbent Assays

Overall performance, including ease of use, total hands-on time, incubation and processing times, sensitivity, and specificity, of each of nine enzyme-linked immunosorbent assays (ELISAs) were compared by using 222 individual fecal samples submitted for the detection ofGiardia lamblia. The assays evaluated were manufactured by Alexon, Inc., Cambridge Biotech Corp., Meridian, Inc., and Trend Scientific, Inc. All assays used polyclonal antibodies except the “new and improved” Microplate (direct and diluted methods) by Alexon, which is a monoclonal antibody assay. Seventy specimens were positive for G. lamblia by ELISA, ova and parasite test, and/or direct fluorescent-antibody assay. One hundred fifty two were negative by all three methods. Sensitivities and specificities ranged from 88.6 to 100% and 99.3 to 100%, respectively. The total hands-on time needed to run one specimen ranged from 1 min to 2 min 17 s per specimen. All except one commercially available ELISA were found to be rapid, sensitive, and specific for the detection of G. lamblia in fecal specimens.

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