scholarly journals Comparison of the Vitek Gram-Positive Susceptibility 106 Card and the MRSA-Screen Latex Agglutination Test for Determining Oxacillin Resistance in Clinical Bloodstream Isolates of Staphylococcus aureus

2001 ◽  
Vol 39 (1) ◽  
pp. 53-56 ◽  
Author(s):  
T. Yamazumi ◽  
S. A. Marshall ◽  
W. W. Wilke ◽  
D. J. Diekema ◽  
M. A. Pfaller ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Gram Positive ◽  
Oxacillin Resistance

scholarly journals Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

2014 ◽  
Vol 52 (9) ◽  
pp. 3394-3398 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Thomas Walther ◽  
Sonja Molinaro ◽  
Xuehua Li ◽  
Guoqing Xia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

Evaluation of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus from Foods

1993 ◽  
Vol 56 (9) ◽  
pp. 759-762 ◽  
Author(s):  
TSUNG C. CHANG ◽  
SU H. HUANG
Keyword(s):  
Staphylococcus Aureus ◽  
Immunoglobulin G ◽  
Protein A ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Polystyrene Latex ◽  
Latex Agglutination Test ◽  
Overnight Incubation ◽  
Latex Test

A latex agglutination kit (AUREUS TEST™) for the rapid identification of Staphylococcus aureus was evaluated in this study. The reagent consists of polystyrene latex particles sensitized with rabbit anti-protein A immunoglobulin G and fibrinogen. Due to the respective binding of bacterial protein A with immunoglobulin G and coagulase with fibrinogen, an agglutination reaction occurs within 1 min when the latex is mixed with a suspension of S. aureus. Of 157 S. aureus isolates (138 from foods) and 110 non-S. aureus isolates (58 species belonging to 19 genera), the sensitivity and specificity of the latex test were 100 and 94.4–100%, respectively. The results were comparable to the conventional coagulase test. Therefore, it is proposed that suspicious colonies of S. aureus on Baird-Parker agar medium be subcultured to tryptic soy agar for overnight incubation. Cultures grown on tryptic soy agar are used for latex agglutination test. The latex agglutination test can be finished within minutes and could be used as an alternative rapid procedure of the coagulase test, which needs several hours or even overnight incubation for completion. In addition, all S. aureus isolates grown on Baird-Parker agar also could be correctly identified by the latex reagent.

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