scholarly journals Evaluation of the Mastalex latex agglutination test for methicillin resistance in Staphylococcus aureus grown on different screening media

2001 ◽  
Vol 47 (2) ◽  
pp. 187-189 ◽  
Author(s):  
D. F. J. Brown
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

scholarly journals Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

1999 ◽  
Vol 37 (9) ◽  
pp. 2789-2792 ◽  
Author(s):  
Arjanne van Griethuysen ◽  
Miranda Pouw ◽  
Nan van Leeuwen ◽  
Max Heck ◽  
Piet Willemse ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Screen Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Specific Test ◽  
Methicillin Resistant ◽  
Phage Types ◽  
Test Specificity

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a inStaphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methicillin-susceptible isolates from cultures of blood from consecutive patients. Also, 267 methicillin-resistant isolates that comprised 248 different phage types were tested. Methicillin resistance was defined as the presence of themecA gene. Of the 267 mecA gene-positive isolates, 263 were positive by the MRSA screen test (sensitivity, 98.5%), and all the mecA-gene negative strains were negative by the MRSA screen test (specificity, 100%). The oxacillin agar screen test detected methicillin resistance in 250 of themecA gene-positive isolates (sensitivity, 93.6%). The sensitivity of the MRSA screen test was statistically significantly higher than the sensitivity of the oxacillin agar screen test (P < 0.05). The MRSA screen test is a highly sensitive and specific test for the detection of methicillin resistance. Also, it offers results within half an hour and is easy to perform, which makes this test a valuable tool in the ongoing battle against methicillin-resistant S. aureus.

scholarly journals Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

2014 ◽  
Vol 52 (9) ◽  
pp. 3394-3398 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Thomas Walther ◽  
Sonja Molinaro ◽  
Xuehua Li ◽  
Guoqing Xia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

scholarly journals Rapid Detection of Methicillin Resistance in Staphylococcus aureus Isolates by the MRSA-Screen Latex Agglutination Test

1999 ◽  
Vol 37 (9) ◽  
pp. 3029-3030 ◽  
Author(s):  
Willem B. van Leeuwen ◽  
Cindy van Pelt ◽  
Ad Luijendijk ◽  
Henri A. Verbrugh ◽  
Wil H. F. Goessens
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Screen Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Negative Results ◽  
Specificity And Sensitivity ◽  
Latex Test

The slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR (“gold standard”) for the detection of methicillin resistance inStaphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a (PBP2a) antigen in 87 of 90 genetically diverse methicillin-resistant S. aureus (MRSA) stock culture strains, leading to a sensitivity of 97%. The three discrepant MRSA strains displayed positive results only after induction of themecA gene by exposure to methicillin. Both mecAPCR and MRSA-Screen displayed negative results among the methicillin-susceptible S. aureus strains (n = 106), as well as for Micrococcus spp. (n = 10), members of the familyEnterobacteriaceae (n = 10),Streptococcus pneumoniae (n = 10), andEnterococcus spp. (n = 10) (specificity = 100%). Producing the same PBP2a antigen, all 10 methicillin-resistant Staphylococcus epidermidis strains score positived in both the latex test and the mecA PCR. Consequently, the MRSA-Screen test should be applied only after identification of the MRSA strain to the species level to rule out coagulase-negative staphylococci. In conclusion, due to excellent specificity and sensitivity the MRSA-Screen latex test has the potential to be successfully used for routine applications in the microbiology laboratory.

Evaluation of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus from Foods

1993 ◽  
Vol 56 (9) ◽  
pp. 759-762 ◽  
Author(s):  
TSUNG C. CHANG ◽  
SU H. HUANG
Keyword(s):  
Staphylococcus Aureus ◽  
Immunoglobulin G ◽  
Protein A ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Polystyrene Latex ◽  
Latex Agglutination Test ◽  
Overnight Incubation ◽  
Latex Test

A latex agglutination kit (AUREUS TEST™) for the rapid identification of Staphylococcus aureus was evaluated in this study. The reagent consists of polystyrene latex particles sensitized with rabbit anti-protein A immunoglobulin G and fibrinogen. Due to the respective binding of bacterial protein A with immunoglobulin G and coagulase with fibrinogen, an agglutination reaction occurs within 1 min when the latex is mixed with a suspension of S. aureus. Of 157 S. aureus isolates (138 from foods) and 110 non-S. aureus isolates (58 species belonging to 19 genera), the sensitivity and specificity of the latex test were 100 and 94.4–100%, respectively. The results were comparable to the conventional coagulase test. Therefore, it is proposed that suspicious colonies of S. aureus on Baird-Parker agar medium be subcultured to tryptic soy agar for overnight incubation. Cultures grown on tryptic soy agar are used for latex agglutination test. The latex agglutination test can be finished within minutes and could be used as an alternative rapid procedure of the coagulase test, which needs several hours or even overnight incubation for completion. In addition, all S. aureus isolates grown on Baird-Parker agar also could be correctly identified by the latex reagent.

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