scholarly journals Induction of Cross-Reactive Antibodies to Novel H7N9 Influenza Virus by Recombinant Newcastle Disease Virus Expressing a North American Lineage H7 Subtype Hemagglutinin

2013 ◽  
Vol 87 (14) ◽  
pp. 8235-8240 ◽  
Author(s):  
P. H. Goff ◽  
F. Krammer ◽  
R. Hai ◽  
C. W. Seibert ◽  
I. Margine ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
North American ◽  
Newcastle Disease ◽  
H7n9 Influenza Virus ◽  
H7n9 Influenza ◽  
H7 Subtype ◽  
Recombinant Newcastle Disease Virus

scholarly journals Recombinant Newcastle Disease Virus as a Vaccine Vector

2001 ◽  
Vol 75 (23) ◽  
pp. 11868-11873 ◽  
Author(s):  
Takaaki Nakaya ◽  
Jerome Cros ◽  
Man-Seong Park ◽  
Yurie Nakaya ◽  
Hongyong Zheng ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Lethal Dose ◽  
Vaccine Vector ◽  
Virus Challenge ◽  
Influenza Virus Hemagglutinin ◽  
Recombinant Newcastle Disease Virus

ABSTRACT A complete cDNA clone of the Newcastle disease virus (NDV) vaccine strain Hitchner B1 was constructed, and infectious recombinant virus expressing an influenza virus hemagglutinin was generated by reverse genetics. The rescued virus induces a strong humoral antibody response against influenza virus and provides complete protection against a lethal dose of influenza virus challenge in mice, demonstrating the potential of recombinant NDV as a vaccine vector.

Protection and Differentiation of Infected from Vaccinated Animals by an Inactivated Recombinant Newcastle Disease Virus/Avian Influenza H5 Vaccine

2010 ◽  
Vol 5 (s1) ◽  
pp. e23-e24
Author(s):  
Bernardo Lozano-Dubernard ◽  
Ernesto Soto-Priante ◽  
David Sarfati-Mizrahi ◽  
Felipa Castro-Peralta ◽  
Ricardo Flores-Castro ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Newcastle Disease Virus

scholarly journals An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 96-100
Author(s):  
Pheik-Sheen Cheow ◽  
Tiong Kit Tan ◽  
Adelene Ai-Lian Song ◽  
Khatijah Yusoff ◽  
Suet Lin Chia
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Vaccine Development ◽  
Transfection Efficiency ◽  
Transfection Method ◽  
Helper Plasmids ◽  
Immunogenic Properties ◽  
Recombinant Newcastle Disease Virus

Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.

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