scholarly journals Terminal amino acid sequences and proteolytic cleavage sites of mouse mammary tumor virus env gene products.

1983 ◽  
Vol 48 (1) ◽  
pp. 314-319 ◽  
Author(s):  
L E Henderson ◽  
R Sowder ◽  
G Smythers ◽  
S Oroszlan
Keyword(s):  
Amino Acid ◽  
Mouse Mammary Tumor Virus ◽  
Amino Acid Sequences ◽  
Gene Products ◽  
Cleavage Sites ◽  
Terminal Amino Acid ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Terminal Amino

scholarly journals Murine tissue amyloid protein AA. NH2-terminal sequence identity with only one of two serum amyloid protein (ApoSAA) gene products.

1984 ◽  
Vol 159 (2) ◽  
pp. 641-646 ◽  
Author(s):  
J S Hoffman ◽  
L H Ericsson ◽  
N Eriksen ◽  
K A Walsh ◽  
E P Benditt
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Gene Products ◽  
Amyloid Protein ◽  
Terminal Sequence ◽  
Terminal Amino Acid ◽  
Sequence Identity ◽  
Murine Tissue ◽  
Terminal Amino ◽  
Acute Phase Serum

Amyloid protein AA is the presumptive fragment of an acute phase serum apolipoprotein, apoSAA. Two major murine apoSAA isotypes (apoSAA1 and apoSAA2) have been identified. The NH2-terminal amino acid sequences of purified murine apoSAA1 and apoSAA2 have been examined and compared with that of murine amyloid protein AA. Our results indicate that apoSAA1 and apoSAA2 are separate gene products and that amyloid protein AA has NH2-terminal amino acid sequence identity with only one of these isotypes, namely apoSAA2.

Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

1968 ◽  
Vol 26 ◽  
pp. 22-23
Author(s):  
N. H. Sarkar ◽  
Dan H. Moore
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Ammonium Acetate ◽  
Dry Weight ◽  
Mouse Tumor ◽  
Mammary Tumor Virus ◽  
Rna Molecules ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

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