scholarly journals 3′-End Stem-Loops of the Subviral RNAs Associated with Turnip Crinkle Virus Are Involved in Symptom Modulation and Coat Protein Binding

2000 ◽  
Vol 74 (14) ◽  
pp. 6528-6537 ◽  
Author(s):  
Jianlong Wang ◽  
Anne E. Simon
Keyword(s):  
Coat Protein ◽  
Turnip Crinkle Virus ◽  
Complementary Strand ◽  
Satellite Rna ◽  
Stem Loop ◽  
Subviral Rnas ◽  
Translation Initiation Codon ◽  
Defective Interfering Rna ◽  
Symptom Modulation ◽  
Interfering Rna

ABSTRACT Many plant RNA viruses are associated with one or more subviral RNAs. Two subviral RNAs, satellite RNA C (satC) and defective interfering RNA G (diG) intensify the symptoms of their helper, turnip crinkle virus (TCV). However, when the coat protein (CP) of TCV was replaced with that of the related Cardamine chlorotic fleck virus (CCFV), both subviral RNAs attenuated symptoms of the hybrid virus TCV-CPCCFV. In contrast, when the translation initiation codon of the TCV CP was altered to ACG and reduced levels of CP were synthesized, satC attenuated symptoms while diG neither intensified nor attenuated symptoms. The determinants for this differential symptom modulation were previously localized to the 3′-terminal 100 bases of the subviral RNAs, which contain six positional differences (Q. Kong, J.-W. Oh, C. D. Carpenter, and A. E. Simon, Virology 238:478–485, 1997). In the current study, we have determined that certain sequences within the 3′-terminal stem-loop structures of satC and diG, which also serve as promoters for complementary strand synthesis, are critical for symptom modulation. Furthermore, the ability to attenuate symptoms was correlated with weakened binding of TCV CP to the hairpin structure.

scholarly journals The Coat Protein of Turnip Crinkle Virus Is Involved in Subviral RNA-Mediated Symptom Modulation and Accumulation

Virology ◽  
1997 ◽  
Vol 238 (2) ◽  
pp. 478-485 ◽  
Author(s):  
Qingzhong Kong ◽  
Jong-Won Oh ◽  
Clifford D Carpenter ◽  
Anne E Simon
Keyword(s):  
Coat Protein ◽  
Turnip Crinkle Virus ◽  
Symptom Modulation ◽  
Subviral Rna

scholarly journals Defective Interfering RNAs of a Satellite Virus

2001 ◽  
Vol 75 (11) ◽  
pp. 5429-5432 ◽  
Author(s):  
Wenping Qiu ◽  
Scholthof G. Karen-Beth
Keyword(s):  
Mosaic Virus ◽  
Rna World ◽  
Rna Virus ◽  
Coding Region ◽  
Satellite Virus ◽  
Rate Of Spread ◽  
Subviral Rnas ◽  
The Family ◽  
Defective Interfering Rna ◽  
Interfering Rna

ABSTRACT Panicum mosaic virus (PMV) is a recently molecularly characterized RNA virus with the unique feature of supporting the replication of two subviral RNAs in a few species of the familyGramineae. The subviral agents include a satellite RNA (satRNA) that is devoid of a coding region and the unrelated satellite panicum mosaic virus (SPMV) that encodes its own capsid protein. Here we report the association of this complex with a new entity in the RNA world, a defective-interfering RNA (DI) of a satellite virus. The specificity of interactions governing this four-component viral system is illustrated by the ability of the SPMV DIs to strongly interfere with the accumulation of the parental SPMV. The SPMV DIs do not interfere with PMV satRNA, but they do slightly enhance the rate of spread and titer of PMV. The SPMV-derived DIs provide an additional avenue by which to investigate fundamental biological questions, including the evolution and interactions of infectious RNAs.

scholarly journals Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein

Virology ◽  
2020 ◽  
Vol 551 ◽  
pp. 64-74
Author(s):  
Chao Wu ◽  
Srinivasaraghavan Kannan ◽  
Chandra S. Verma ◽  
Kunchithapadam Swaminathan ◽  
Sek-Man Wong
Keyword(s):  
Molecular Modeling ◽  
Coat Protein ◽  
Sulfite Oxidase ◽  
Turnip Crinkle Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Cymbidium Ringspot Virus Harnesses RNA Silencing To Control the Accumulation of Virus Parasite Satellite RNA

2008 ◽  
Vol 82 (23) ◽  
pp. 11851-11858 ◽  
Author(s):  
Vitantonio Pantaleo ◽  
József Burgyán
Keyword(s):  
Rna Silencing ◽  
Sequence Similarity ◽  
Helper Virus ◽  
Ringspot Virus ◽  
Silencing Suppressor ◽  
Short Interfering Rna ◽  
Satellite Rna ◽  
The Cost ◽  
Interfering Rna ◽  
Rna Replicon

ABSTRACT Cymbidium ringspot virus (CymRSV) satellite RNA (satRNA) is a parasitic subviral RNA replicon that replicates and accumulates at the cost of its helper virus. This 621-nucleotide (nt) satRNA species has no sequence similarity to the helper virus, except for a 51-nt-long region termed the helper-satellite homology (HSH) region, which is essential for satRNA replication. We show that the accumulation of satRNA strongly depends on temperature and on the presence of the helper virus p19 silencing suppressor protein, suggesting that RNA silencing plays a crucial role in satRNA accumulation. We also demonstrate that another member of the Tombusvirus genus, Carnation Italian ringspot virus (CIRV), supports satRNA accumulation at a higher level than CymRSV. Our results suggest that short interfering RNA (siRNA) derived from CymRSV targets satRNA more efficiently than siRNA from CIRV, possibly because of the higher sequence similarity between the HSH regions of the helper and CIRV satRNAs. RNA silencing sensor RNA carrying the putative satRNA target site in the HSH region was efficiently cleaved when transiently expressed in CymRSV-infected plants but not in CIRV-infected plants. Strikingly, replacing the CymRSV HSH box2 sequence with that of CIRV restores satRNA accumulation both at 24°C and in the absence of the p19 suppressor protein. These findings demonstrate the extraordinary adaptation of this virus to its host in terms of harnessing the antiviral silencing response of the plant to control the virus parasite satRNA.

scholarly journals Expression of Interferon-γ by a Coronavirus Defective-Interfering RNA Vector and Its Effect on Viral Replication, Spread, and Pathogenicity

Virology ◽  
1997 ◽  
Vol 233 (2) ◽  
pp. 327-338 ◽  
Author(s):  
Xuming Zhang ◽  
David R. Hinton ◽  
Daniel J. Cua ◽  
Stephen A. Stohlman ◽  
Michael M.C. Lai
Keyword(s):  
Viral Replication ◽  
Interferon Γ ◽  
Defective Interfering Rna ◽  
Interfering Rna

A defective interfering RNA that contains a mosaic of a plant virus genome

Cell ◽  
1987 ◽  
Vol 51 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Bradley I. Hillman ◽  
James C. Carrington ◽  
Thomas J. Morris
Keyword(s):  
Plant Virus ◽  
Virus Genome ◽  
Defective Interfering Rna ◽  
Interfering Rna
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