RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.
1990 ◽
Vol 10
(10)
◽
pp. 5575-5579
◽
Keyword(s):
Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles of heat denaturation and renaturation of RNA to achieve a high efficiency of cleavage. This effect can also be achieved by gradual denaturation of RNA with heat or formamide. These results suggest that only a subpopulation of the catalytic RNA molecules assumes the active conformation required for self-cleavage. This procedure could be of general use for detecting catalytic RNA activities.
1990 ◽
Vol 10
(10)
◽
pp. 5575-5579
Keyword(s):
2002 ◽
Vol 99
(23)
◽
pp. 15118-15123
◽
1997 ◽
Vol 247
(3)
◽
pp. 741-753
◽
1992 ◽
Vol 223
(1)
◽
pp. 233-245
◽
1994 ◽
Vol 32
(4)
◽
pp. 1112-1114
Keyword(s):