scholarly journals A Drosophila ribosomal protein functions in mammalian cells.

1990 ◽  
Vol 10 (9) ◽  
pp. 4524-4528 ◽  
Author(s):  
C G Maki ◽  
D D Rhoads ◽  
J J Diaz ◽  
D J Roufa
Keyword(s):  
Ribosomal Protein ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mammalian Cells ◽  
Expression Vector ◽  
Resistance Mutation ◽  
Chinese Hamster ◽  
Ribosomal Subunits ◽  
Cdna Expression ◽  
Protein Functions

A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.

A Drosophila ribosomal protein functions in mammalian cells

1990 ◽  
Vol 10 (9) ◽  
pp. 4524-4528
Author(s):  
C G Maki ◽  
D D Rhoads ◽  
J J Diaz ◽  
D J Roufa
Keyword(s):  
Ribosomal Protein ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mammalian Cells ◽  
Expression Vector ◽  
Resistance Mutation ◽  
Chinese Hamster ◽  
Ribosomal Subunits ◽  
Cdna Expression ◽  
Protein Functions

A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles

2021 ◽  
pp. 2100098
Author(s):  
Benjamin F. Synoground ◽  
Claire E. McGraw ◽  
Kathryn S. Elliott ◽  
Christina Leuze ◽  
Jada R. Roth ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Ammonia Stress ◽  
Igg Glycosylation

Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells

2008 ◽  
Vol 369 (2) ◽  
pp. 712-717 ◽  
Author(s):  
Oleg V. Yarishkin ◽  
Eun-Mi Hwang ◽  
Jae-Yong Park ◽  
Dawon Kang ◽  
Jaehee Han ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster

Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells

2002 ◽  
Vol 79 (5) ◽  
pp. 580-585 ◽  
Author(s):  
Elisabetta G. P. Prati ◽  
Mattia Matasci ◽  
Tobias B. Suter ◽  
Andre Dinter ◽  
Adriana R. Sburlati ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Down Regulation ◽  
Glycosylation Pathway
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