Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells

2002 ◽  
Vol 79 (5) ◽  
pp. 580-585 ◽  
Author(s):  
Elisabetta G. P. Prati ◽  
Mattia Matasci ◽  
Tobias B. Suter ◽  
Andre Dinter ◽  
Adriana R. Sburlati ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Down Regulation ◽  
Glycosylation Pathway

Engineering of coordinated up- and down-regulation of two glycosyltransferases of the o-glycosylation pathway in Chinese hamster ovary (CHO) cells

2000 ◽  
Vol 68 (3) ◽  
pp. 239-244 ◽  
Author(s):  
Elisabetta G.P. Prati ◽  
Mattia Matasci ◽  
Tobias B. Suter ◽  
Andre Dinter ◽  
Adriana R. Sburlati ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Down Regulation ◽  
Glycosylation Pathway

scholarly journals β3-adrenoceptor agonist-induced down-regulation of Gsα and functional desensitization in a Chinese hamster ovary cell line expressing a β3-adrenoceptor refractory to down-regulation

1994 ◽  
Vol 303 (3) ◽  
pp. 973-978 ◽  
Author(s):  
J Chambers ◽  
J Park ◽  
D Cronk ◽  
C Chapman ◽  
F R Kennedy ◽  
...  
Keyword(s):  
G Protein ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Maximal Effect ◽  
Down Regulation ◽  
Adrenoceptor Agonist ◽  
Fold Reduction ◽  
Beta 2 ◽  
Gs Alpha

Chinese hamster ovary (CHO) cells transfected to express human beta 2- or beta 3-adrenoceptors (beta 2-CHO and beta 3-CHO cells) were exposed to the beta-adrenoceptor agonist isoprenaline at various concentrations and for differing times. Sustained exposure of the beta 2-CHO but not beta 3-CHO cells to isoprenaline resulted in a time- and concentration-dependent down-regulation of the receptor as measured by a reduction in specific binding of [125I]cyanopindolol. Such maintained exposure of cells expressing either receptor to the agonist produced a marked down-regulation of immunologically detectable levels of the alpha subunit of the stimulatory guanine-nucleotide-binding protein Gs. This effect was specific for Gs because levels of both G12 alpha and Gq alpha/G11 alpha were unaltered by isoprenaline treatment of both beta 2-CHO and beta 3-CHO cells. The effect of isoprenaline on Gs alpha down-regulation was some 30-fold more potent in the beta 2-CHO than in the beta 3-CHO cells. Time courses of isoprenaline-induced down-regulation of Gs alpha were not different, however, in the two cell lines. Isoprenaline treatment of the beta 3-CHO cells produced a desensitization of agonist-mediated regulation of adenylyl cyclase, manifested by a 4-fold reduction in the potency and a 30% reduction in maximal effect of the agonist, whereas desensitization of the beta 2-CHO cells was considerably greater (25-fold reduction in potency and 70% reduction in maximal effect). These results demonstrate that agonist-induced down-regulation of the G-protein which interacts with a receptor can be produced by both beta 2- and beta 3-adrenoceptors. Despite apparent concurrence of down-regulation of receptors and G-proteins in other systems [e.g. Adie, Mullaney, McKenzie and Milligan (1992) Biochem. J. 285, 529-536], agonist-induced receptor down-regulation does not appear to be a prerequisite for down-regulation of the G-protein. Furthermore, the results suggest that agonist-induced down-regulation of a G-protein may be sufficient, in the absence of receptor regulation, to induce some agonist desensitization of effector function.

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles

2021 ◽  
pp. 2100098
Author(s):  
Benjamin F. Synoground ◽  
Claire E. McGraw ◽  
Kathryn S. Elliott ◽  
Christina Leuze ◽  
Jada R. Roth ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Ammonia Stress ◽  
Igg Glycosylation

Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells

2008 ◽  
Vol 369 (2) ◽  
pp. 712-717 ◽  
Author(s):  
Oleg V. Yarishkin ◽  
Eun-Mi Hwang ◽  
Jae-Yong Park ◽  
Dawon Kang ◽  
Jaehee Han ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster

Toxicity to Chinese hamster ovary (CHO) cells of the products of reaction of butylated hydroxyanisole with nitrite at low pH

1994 ◽  
Vol 8 (1) ◽  
pp. 117-123
Author(s):  
B.J. Phillips ◽  
A.C. Tee ◽  
P.A. Carroll ◽  
R. Purchase ◽  
D.G. Walters
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Low Ph ◽  
Butylated Hydroxyanisole ◽  
Products Of Reaction
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