Gene Transfer by Retrovirus Vectors Occurs Only in Cells That Are Actively Replicating at the Time of Infection

1992 ◽  
Vol 12 (1) ◽  
pp. 433-433
Keyword(s):  
Gene Transfer ◽  
Time Of Infection ◽  
In Cells

What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues

2008 ◽  
Vol 41 (3) ◽  
pp. 286-295 ◽  
Author(s):  
Jean-Michel Escoffre ◽  
Thomas Portet ◽  
Luc Wasungu ◽  
Justin Teissié ◽  
David Dean ◽  
...  
Keyword(s):  
Gene Transfer ◽  
In Cells

Gene transfer in situ and in cells for intracerebral transplantation

1993 ◽  
Vol 5 (6) ◽  
pp. 453-459 ◽  
Author(s):  
Philippe Horellou ◽  
Cecilia Lundberg ◽  
Jean-Jacques Robert ◽  
Anders Björklund ◽  
Jacques Mallet
Keyword(s):  
Gene Transfer ◽  
Intracerebral Transplantation ◽  
In Cells

Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection

1990 ◽  
Vol 10 (8) ◽  
pp. 4239-4242 ◽  
Author(s):  
D G Miller ◽  
M A Adam ◽  
A D Miller
Keyword(s):  
Gene Transfer ◽  
Stationary Phase ◽  
Retroviral Vectors ◽  
Rat Embryo ◽  
Retroviral Infection ◽  
Replication Competent Virus ◽  
Retrovirus Vector ◽  
Time Of Infection ◽  
Infected Cells ◽  
Retrovirus Infection

Previous reports have shown that retrovirus infection is inhibited in nonreplicating (stationary-phase [hereafter called stationary]) cells. Infection of stationary cells was shown to occur when the cells were allowed to replicate at times up to a week after infection, suggesting that an unintegrated retrovirus could persist in a form that was competent to integrate after release of the block to replication. However, those studies were complicated by the use of replication-competent virus, which can spread in the infected cells. We have used a replication-defective retrovirus vector to compare the efficiency of gene transfer in stationary and replicating rat embryo fibroblasts. In agreement with previous results, gene transfer was inhibited 100-fold in stationary versus replicating cells. In contrast to previously reported results, the block to infection could not be relieved by stimulating stationary cells to divide at times from 6 h to 10 days after infection. Thus, for successful retroviral infection, the infected cells must be replicating at the time of infection. These results have important implications for the use of retroviral vectors for gene transfer.

scholarly journals Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.

1990 ◽  
Vol 10 (8) ◽  
pp. 4239-4242 ◽  
Author(s):  
D G Miller ◽  
M A Adam ◽  
A D Miller
Keyword(s):  
Gene Transfer ◽  
Stationary Phase ◽  
Retroviral Vectors ◽  
Rat Embryo ◽  
Retroviral Infection ◽  
Replication Competent Virus ◽  
Retrovirus Vector ◽  
Time Of Infection ◽  
Infected Cells ◽  
Retrovirus Infection

Previous reports have shown that retrovirus infection is inhibited in nonreplicating (stationary-phase [hereafter called stationary]) cells. Infection of stationary cells was shown to occur when the cells were allowed to replicate at times up to a week after infection, suggesting that an unintegrated retrovirus could persist in a form that was competent to integrate after release of the block to replication. However, those studies were complicated by the use of replication-competent virus, which can spread in the infected cells. We have used a replication-defective retrovirus vector to compare the efficiency of gene transfer in stationary and replicating rat embryo fibroblasts. In agreement with previous results, gene transfer was inhibited 100-fold in stationary versus replicating cells. In contrast to previously reported results, the block to infection could not be relieved by stimulating stationary cells to divide at times from 6 h to 10 days after infection. Thus, for successful retroviral infection, the infected cells must be replicating at the time of infection. These results have important implications for the use of retroviral vectors for gene transfer.

Gene Transfer of the Catabolic Inhibitor TIMP-1 Increases Measured Proteoglycans in Cells from Degenerated Human Intervertebral Discs

Spine ◽  
2003 ◽  
Vol 28 (20) ◽  
pp. 2331-2337 ◽  
Author(s):  
Corey J. Wallach ◽  
Satoshi Sobajima ◽  
Yasuhiko Watanabe ◽  
Joseph S. Kim ◽  
Helga I. Georgescu ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Intervertebral Discs ◽  
In Cells

Atelocollagen-Based Gene Transfer in Cells Allows High-Throughput Screening of Gene Functions

2001 ◽  
Vol 289 (5) ◽  
pp. 1075-1081 ◽  
Author(s):  
Kimi Honma ◽  
Takahiro Ochiya ◽  
Shunji Nagahara ◽  
Akihiko Sano ◽  
Hanako Yamamoto ◽  
...  
Keyword(s):  
Gene Transfer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Gene Functions ◽  
In Cells
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