What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues

2008 ◽  
Vol 41 (3) ◽  
pp. 286-295 ◽  
Author(s):  
Jean-Michel Escoffre ◽  
Thomas Portet ◽  
Luc Wasungu ◽  
Justin Teissié ◽  
David Dean ◽  
...  
Keyword(s):  
Gene Transfer ◽  
In Cells

Gene transfer in situ and in cells for intracerebral transplantation

1993 ◽  
Vol 5 (6) ◽  
pp. 453-459 ◽  
Author(s):  
Philippe Horellou ◽  
Cecilia Lundberg ◽  
Jean-Jacques Robert ◽  
Anders Björklund ◽  
Jacques Mallet
Keyword(s):  
Gene Transfer ◽  
Intracerebral Transplantation ◽  
In Cells

Gene Transfer of the Catabolic Inhibitor TIMP-1 Increases Measured Proteoglycans in Cells from Degenerated Human Intervertebral Discs

Spine ◽  
2003 ◽  
Vol 28 (20) ◽  
pp. 2331-2337 ◽  
Author(s):  
Corey J. Wallach ◽  
Satoshi Sobajima ◽  
Yasuhiko Watanabe ◽  
Joseph S. Kim ◽  
Helga I. Georgescu ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Intervertebral Discs ◽  
In Cells

Atelocollagen-Based Gene Transfer in Cells Allows High-Throughput Screening of Gene Functions

2001 ◽  
Vol 289 (5) ◽  
pp. 1075-1081 ◽  
Author(s):  
Kimi Honma ◽  
Takahiro Ochiya ◽  
Shunji Nagahara ◽  
Akihiko Sano ◽  
Hanako Yamamoto ◽  
...  
Keyword(s):  
Gene Transfer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Gene Functions ◽  
In Cells

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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