scholarly journals Fission Yeast Cdc24 Is a Replication Factor C- and Proliferating Cell Nuclear Antigen-Interacting Factor Essential for S-Phase Completion

1999 ◽  
Vol 19 (2) ◽  
pp. 1038-1048 ◽  
Author(s):  
Hiroyuki Tanaka ◽  
Koichi Tanaka ◽  
Hiroshi Murakami ◽  
Hiroto Okayama
Keyword(s):  
Dna Replication ◽  
Fission Yeast ◽  
Proliferating Cell Nuclear Antigen ◽  
Large Subunit ◽  
Nuclear Antigen ◽  
Temperature Sensitive ◽  
Replication Factor C ◽  
Replication Factor ◽  
Factor C ◽  
Proliferating Cell

ABSTRACT At the nonpermissive temperature the fission yeastcdc24-M38 mutant arrests in the cell cycle with incomplete DNA replication as indicated by pulsed-field gel electrophoresis. Thecdc24 + gene encodes a 501-amino-acid protein with no significant homology to any known proteins. The temperature-sensitive cdc24 mutant is effectively rescued by pcn1 +, rfc1 + (a fission yeast homologue of RFC1), andhhp1 +, which encode the proliferating cell nuclear antigen (PCNA), the large subunit of replication factor C (RFC), and a casein kinase I involved in DNA damage repair, respectively. The Cdc24 protein binds PCNA and RFC1 in vivo, and the domains essential for Cdc24 function and for RFC1 and PCNA binding colocalize in the N-terminal two-thirds of the molecule. In addition,cdc24 + genetically interacts with the gene encoding the catalytic subunit of DNA polymerase ɛ, which is stimulated by PCNA and RFC, and with those encoding the fission yeast counterparts of Mcm2, Mcm4, and Mcm10. These results indicate that Cdc24 is an RFC- and PCNA-interacting factor required for DNA replication and might serve as a target for regulation.

scholarly journals Synthetic Activity ofSsoDNA Polymerase Y1, an Archaeal DinB-like DNA Polymerase, Is Stimulated by Processivity Factors Proliferating Cell Nuclear Antigen and Replication Factor C

2001 ◽  
Vol 276 (50) ◽  
pp. 47394-47401 ◽  
Author(s):  
Petr Grúz ◽  
Francesca M. Pisani ◽  
Masatomi Shimizu ◽  
Masami Yamada ◽  
Ikuko Hayashi ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Dna Polymerases ◽  
Nuclear Antigen ◽  
Synthetic Activity ◽  
Replication Factor C ◽  
Replication Factor ◽  
Pol Iv ◽  
Factor C ◽  
Proliferating Cell

DNA replication efficiency is dictated by DNA polymerases (pol) and their associated proteins. The recent discovery of DNA polymerase Y family (DinB/UmuC/RAD30/REV1 superfamily) raises a question of whether the DNA polymerase activities are modified by accessory proteins such as proliferating cell nuclear antigen (PCNA). In fact, the activity of DNA pol IV (DinB) ofEscherichia coliis enhanced upon interaction with the β subunit, the processivity factor of DNA pol III. Here, we report the activity ofSsoDNA pol Y1 encoded by thedbhgene of the archaeonSulfolobus solfataricusis greatly enhanced by the presence of PCNA and replication factor C (RFC).Ssopol Y1per sewas a distributive enzyme but a substantial increase in the processivity was observed on poly(dA)-oligo(dT) in the presence of PCNA (039p or 048p) and RFC. The length of the synthesized DNA product reached at least 200 nucleotides.Ssopol Y1 displayed a higher affinity for DNA compared with pol IV ofE. coli, suggesting that the two DNA polymerases have distinct reason(s) to require the processivity factors for efficient DNA synthesis. The abilities of pol Y1 and pol IV to bypass DNA lesions and their sensitive sites to protease are also discussed.

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