A Union of Dynamic Hydrological Modeling and Satellite Remotely-Sensed Data for Spatiotemporal Assessment of Sediment Yields

(1) The existing frameworks for water quality modeling overlook the connection between multiple dynamic factors affecting spatiotemporal sediment yields (SY). This study aimed to implement satellite remotely sensed data and hydrological modeling to dynamically assess the multiple factors within basin-scale hydrologic models for a realistic spatiotemporal prediction of SY in watersheds. (2) A connective algorithm was developed to incorporate dynamic models of the crop and cover management factor (C-factor) and the soil erodibility factor (K-factor) into the Soil and Water Assessment Tool (SWAT) with the aid of the Python programming language and Geographic Information Systems (GIS). The algorithm predicted the annual SY in each hydrologic response unit (HRU) of similar land cover, soil, and slope characteristics in watersheds between 2002 and 2013. (3) The modeled SY closely matched the observed SY using the connective algorithm with the inclusion of the two dynamic factors of K and C (predicted R2 (PR2): 0.60–0.70, R2: 0.70–0.80, Nash Sutcliffe efficiency (NS): 0.65–0.75). The findings of the study highlight the necessity of excellent spatial and temporal data in real-time hydrological modeling of catchments.

Association of rheumatoid arthritis and its severity with human leukocytic antigen-DRB1 alleles in Kurdish region in North of Iraq

Background Rheumatoid arthritis is a complex multifactorial chronic disease, the importance of human leukocytic antigen (HLA) as a major genetic risk factor for rheumatoid arthritis was studied worldwide. The objective of this study is to identify the association of HLA-DRB1 subtypes with rheumatoid arthritis and its severity in Kurdish region. Methods A case–control study recruited 65 rheumatoid arthritis patients and 100 healthy individuals as control group all over the Kurdistan region/Iraq. Both patient and control groups are genotyped using polymerase chain reaction with sequence specific primer. Anti-CCP antibodies were measured by ELISA test. Rheumatoid factor, C-reactive protein, and disease activity score 28 which measured by DAS-28 values were calculated. The DAS-28 was used to assess the clinical severity of the patients. Results HLA-DRB1-0404 and HLA-DRB1-0405 frequencies showed a strong association with disease susceptibility (P < 0.001). The frequency of HLA-DRB1-0411 and HLA-DRB1-0413 were significantly higher in control group (P < 0.001). The frequency of rheumatoid factor and Anti-CCP were significantly higher among shared epitope-positive patients compared to shared epitope-negative patients (P < 0.001). Regarding the disease activity by DAS-28, rheumatoid arthritis patients didn’t show significant difference between the shared epitope-positive and shared epitope-negative patients. Conclusions HLA-DR0404 and HLA-DR0405 alleles are related to RA, while HLA-DR1-0411 and HLA-DRB1-0413 protect against RA in the Kurdistan region in the North of Iraq.

Matrix metalloproteinase-7 induces E-cadherin cleavage in acid-exposed primary human pharyngeal epithelial cells via the ROS/ERK/c-Jun pathway

Laryngopharyngeal reflux disease (LPRD) is caused by pharyngeal mucosal damage due to the reflux of gastric contents, including acid, pepsin, and bile juice. Our previous study has demonstrated that LPRD is associated with the cleavage of E-cadherin, which is facilitated by the acid-activated matrix metalloproteinase-7 (MMP-7); however, the mechanism by which the acid activates MMP-7 remains unclear. The purpose of this study was to investigate the mechanism by which MMP-7 is activated in the pharyngeal epithelial cells that are exposed to acid. The levels of reactive oxygen species (ROS) were measured in the epithelial cells exposed to acid. To investigate the signaling mechanism of ROS in the expression of MMP-7, the mechanism of action of the mitogen-activated protein kinase was examined. The expression of various signaling factors was determined, according to the presence or absence of each inhibitor in the acid-exposed pharyngeal epithelial cells. To identify changes in the cleavage of E-cadherin, the integrity of the mucosal membrane was assessed using a transepithelial permeability test. We found that acid exposure increased the levels of ROS, phosphorylated-extracellular signal-regulated kinase (p-ERK) 1/2, and phosphorylated-c-Jun (p–c-Jun) in pharyngeal epithelial cells. The ROS inhibitor reduced the expression of p-ERK and MMP-7, while the ERK inhibitor reduced the expression of p–c-Jun and MMP-7. Moreover, the c-Jun inhibitor reduced the expression of MMP-7 and blocked the degradation of E-cadherin. In addition, decrease in the levels of immunostained E-cadherin and increase in transepithelial permeability after acid exposure were collectively alleviated by the inhibitors of ROS, ERK, and c-Jun. The degradation of E-cadherin that occurs after human mucosal cells are exposed to acid appears to be caused by an increase in the expression of MMP-7 via the ROS/ERK/c-Jun pathway, which is thought to be an important mechanism associated with the development of LPRD. Key messages • ROS is triggered when reflux occurs. • ROS regulates the transcription factor c-Jun via the ERK pathway. • The increase in MMP-7 that induces LPRD is induced via the ROS/ERK/c-Jun pathway. • This study revealed for the first time the expression mechanism of MMP-7, which is one of the causes of LPRD.

Relationship of personal factors and hardiness in students

Problem and purpose. The issue of resilience is very urgent today. Rapid social, economic, political changes in society, the speed of technical and technological changes affect the psychological health of a person, provoking stress, neuroses, inappropriate behavior, depressive states. The study of the relationship of resilience and its components with personality traits will serve as the basis for creating programs for the development of resilience, through the formation of personal characteristics of students necessary for harmonious development and professional self-realization in society. The purpose of the article is to identify and describe the relationship between personality traits and resilience in students. Materials and methods. The study involved 50 students between the ages of 20 and 25 of The Herzen State Pedagogical University of Russia. The study was conducted from October to December 2020 (during the COVID-19 Pandemic) and was carried out remotely. using a Google form. The following methods were used in the study: Hardiness Survey (Hardiness Survey, S. Maddy), 5PFQ personality questionnaire, Multifactorial personality questionnaire 16PF R. Cattell, T.V. Kornilova New questionnaire uncertainty tolerance. To process the data obtained, methods of quantitative and qualitative analysis were used; descriptive statistics methods, r-Spearman rank correlation coefficient. Results. The results of a theoretical and empirical study of hardiness and its relationship with personal factors in students of a pedagogical university are presented. Statistical data are presented, on the basis of which the relationship of hardiness and its components with communicative, intellectual, emotional and regulatory properties of a person is revealed. The study revealed a statistically significant positive relationship between the scale "Resilience" and such indicators of the Multifactorial Personality Questionnaire 16PF as factor A: "isolation-sociability" (r = 0.28; p≤0.05), factor C: "emotional instability-emotional stability "(r = 0.64; p≤0.05), and etc. Conclusion. It was found that hardiness and its components are positively associated with communicative factors (sociability, courage, that is, the activity of social communication, gullibility, plasticity and dominance in social contacts), intellectual factors (plasticity, radicalism, curiosity, that is, flexibility and efficiency of thinking), emotional factors (emotional stability, restraint, calmness and relaxation, expressiveness, that is, expressiveness, sensitivity) and regulatory factors (self-control, responsibility). The solution to the research problem is proposed in the form of creating and implementing a practice-oriented program for the development of hardiness in students.

A Kidney-Targeted Nanoparticle to Augment Renal Lymphatic Density Decreases Blood Pressure in Hypertensive Mice

Chronic interstitial inflammation and renal infiltration of activated immune cells play an integral role in hypertension. Lymphatics regulate inflammation through clearance of immune cells and excess interstitial fluid. Previously, we demonstrated increasing renal lymphangiogenesis prevents hypertension in mice. We hypothesized that targeted nanoparticle delivery of vascular endothelial growth factor-C (VEGF-C) to the kidney would induce renal lymphangiogenesis, lowering blood pressure in hypertensive mice. A kidney-targeting nanoparticle was loaded with a VEGF receptor-3-specific form of VEGF-C and injected into mice with angiotensin II-induced hypertension or LNAME-induced hypertension every 3 days. Nanoparticle-treated mice exhibited increased renal lymphatic vessel density and width compared to hypertensive mice injected with VEGF-C alone. Nanoparticle-treated mice exhibited decreased systolic blood pressure, decreased pro-inflammatory renal immune cells, and increased urinary fractional excretion of sodium. Our findings demonstrate that pharmacologically expanding renal lymphatics decreases blood pressure and is associated with favorable alterations in renal immune cells and increased sodium excretion.

Diagnostic Value of Vascular Endothelial Growth Factor C (VEGF-C) and CA 15-3 in Breast Cancer

ABSTRACT Background: expression of VEGF-C and CA 15-3 may be useful to differentiate between malignant and benign breast tumour because VEGF-C plays a role in promoting angiogenesis and lymphangiogenesis in malignant processes and CA 15-3 is the soluble form of transmembrane protein MUC1, a tumour marker which shows higher expression in breast cancer.Objective: to determine the diagnostic value of VEGF-C and CA 15-3 as tumour markers in patients with breast cancer.Methods: this diagnostic study recruited 76 patients that underwent surgical biopsy procedures at Dr. Kariadi and Pantiwilasa Citarum Hospitals Semarang. The VEGF-C and CA 15-3 levels in blood specimens taken before surgical biopsy procedure was determined using ELISA method. An ROC curve and AUC were used to establish the cut-off points and diagnostic value. Pathology examination results from the biopsy specimens were used as the gold standard.Results: the cut-off value for VEGF-C and CA 15-3 were 989.50 pg/mL and 74.00 U/mL. Sensitivity for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 were 76.6%, 64.1% and 89.1%. Specificity for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 were 75.0%, 75.0% and 50.0%. The AUC for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 was 0.831 (95% CI = 0.727-0.934), 0.742 (95% CI = 0.628-0.856) and 0.840 (95% CI = 0.742-0.938).Conclusion: VEGF-C in combination with CA 15-3 is the best diagnostic parameter for breast cancer and has the best accuracy as a tumour marker for breast cancer.

Vascular endothelial growth factor-c regulates hematopoietic stem cell fate in the dorsal aorta

Hematopoietic stem and progenitor cells (HSPCs) are multipotent cells that self-renew or differentiate to establish the entire blood hierarchy. HSPCs arise from the hemogenic endothelium of the dorsal aorta (DA) during development in a process called endothelial-to-hematopoietic transition. The factors and signals that control HSPC fate decisions from the hemogenic endothelium are not fully understood. We found that vegfc has a role in HSPC emergence from the zebrafish DA. Using time-lapse live imaging, we show that some HSPCs in the DA of vegfc loss-of-function embryos display altered cellular behavior. Instead of typical budding from the DA, emergent HSPCs exhibit crawling behavior similar to myeloid cells. This was confirmed by increased myeloid cell marker expression in the ventral wall of the DA and the caudal hematopoietic tissue. This increase in myeloid cells corresponded with a decrease in HSPCs that persisted into larval stages. Together, our data suggests vegfc regulates HSPC emergence in the hemogenic endothelium, in part by suppressing a myeloid cell fate. Our study provides a potential signal for modulation of HSPC fate in stem cell differentiation protocols.

Towards the Assessment of Soil-Erosion-Related C-Factor on European Scale Using Google Earth Engine and Sentinel-2 Images

Soil erosion is a constant environmental threat for the entirety of Europe. Numerous studies have been published during the last years concerning assessing soil erosion utilising Remote Sensing (RS) and Geographic Information Systems (GIS). Such studies commonly employ empirical erosion models to estimate soil loss on various spatial scales. In this context, empirical models have been highlighted as major approaches to estimate soil loss on various spatial scales. Most of these models analyse environmental factors representing soil-erosion-influencing conditions such as the climate, topography, soil regime, and surface vegetation coverage. In this study, the Google Earth Engine (GEE) cloud computing platform and Sentinel-2 satellite imagery data have been combined to assess the vegetation-coverage-related factor known as cover management factor (C-factor) at a high spatial resolution (10 m) considering a total of 38 European countries. Based on the employment of the RS derivative of the Normalised Difference Vegetation Index (NDVI) for January and December 2019, a C-factor map was generated due to mean annual estimation. National values were then calculated in terms of different types of agricultural land cover classes. Furthermore, the European C-factor (CEUROPE) values concerning the island of Crete (Greece) were compared with relevant values estimated for the island (CCRETE) based on Sentinel-2 images being individually selected at a monthly time-step of 2019 to generate a series of 12 maps for the C-factor in Crete. Our results yielded identical C-factor values for the different approaches. The outcomes denote GEE’s high analytic and processing abilities to analyse massive quantities of data that can provide efficient digital products for soil-erosion-related studies.

C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100A8 and S100A9

The proinflammatory alarmins S100A8 and S100A9 are among the most abundant proteins in neutrophils and monocytes but completely silenced after differentiation to macrophages. The molecular mechanisms of the extraordinarily dynamic transcriptional regulation of s100a8 and s100a9 genes, however, are only barely understood. Using an unbiased genome-wide CRISPR/Cas9 knockout based screening approach in immortalized murine monocytes we identified the transcription factor C/EBPδ as a central regulator of S100A8 and S100A9 expression. S100a8 and S100a9 expression was further controlled by the C/EBPδ-antagonists ATF3 and FBXW7. We confirmed the clinical relevance of this regulatory network in subpopulations of human monocytes in a clinical cohort of cardiovascular patients. Moreover, we identified specific C/EBPδ-binding sites within s100a8 and s100a9 promoter regions, and demonstrated that C/EBPδ-dependent JMJD3-mediated demethylation of H3K27me3 is indispensable for their expression. Overall, our work uncovered C/EBPδ as a novel regulator of S100A8 and S100A9 expression. Therefore, C/EBPδ represents a promising target for modulation of inflammatory conditions that are characterised by S100A8 and S100A9 overexpression.