scholarly journals Complete Genome Sequence of Tetracycline-Resistant Serratia liquefaciens S1, Isolated from Mixed Greens, Obtained Using Illumina MiSeq and Oxford Nanopore MinION Sequencing

2020 ◽  
Vol 9 (19) ◽  
Author(s):  
Maria Stein ◽  
Erik Brinks ◽  
Jana Rathje ◽  
Gyu-Sung Cho ◽  
Charles M. A. P. Franz

Serratia liquefaciens strain S1 was isolated from mixed greens and sequenced with short-read Illumina MiSeq technology and long-read MinION technology. Hybrid sequence assembly generated a complete single circular chromosome and two extrachromosomal contigs, which together encoded 5,098 proteins, 85 tRNAs, and 22 rRNAs.

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Natsuki Tomariguchi ◽  
Kentaro Miyazaki

Rubrobacter xylanophilus strain AA3-22, belonging to the phylum Actinobacteria, was isolated from nonvolcanic Arima Onsen (hot spring) in Japan. Here, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.


2020 ◽  
Vol 9 (21) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Apirak Wiseschart ◽  
Kusol Pootanakit ◽  
Kei Kitahara

ABSTRACT We isolated the novel strain Vibrio rotiferianus AM7 from the shell of an abalone. In this article, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.


2019 ◽  
Vol 8 (45) ◽  
Author(s):  
Hiroki Yu ◽  
Makoto Taniguchi ◽  
Kazuma Uesaka ◽  
Apirak Wiseschart ◽  
Kusol Pootanakit ◽  
...  

Staphylococcus arlettae is one coagulase-negative species in the bacterial genus Staphylococcus. Here, we describe the closed complete genome sequence of S. arlettae strain P2, which was obtained using a hybrid approach combining Oxford Nanopore long-read and Illumina MiSeq short-read sequencing data.


2020 ◽  
Vol 9 (32) ◽  
Author(s):  
Sohaib Sarfraz ◽  
Saïd Oulghazi ◽  
Jérémy Cigna ◽  
Shahbaz Talib Sahi ◽  
Kashif Riaz ◽  
...  

ABSTRACT Pectobacterium punjabense is a newly described species causing blackleg disease in potato plants. Therefore, by the combination of long (Oxford Nanopore Technologies, MinION) and short (Illumina MiSeq) reads, we sequenced the complete genome of P. punjabense SS95T, which contains a circular chromosome of 4.793 Mb with a GC content of 50.7%.


2016 ◽  
Vol 6 (8) ◽  
Author(s):  
Kazuma Nakano ◽  
Maiko Minami ◽  
Misuzu Shinzato ◽  
Makiko Shimoji ◽  
Noriko Ashimine ◽  
...  

ABSTRACT Lactococcus lactis subsp. lactis G50 is a strain with immunostimulating activity, isolated from Napier grass ( Pennisetum purpureum ). We determined the complete genome sequence of this strain using the PacBio RS II platform. The single circular chromosome consists of 2,346,663 bp, with 35.03% G+C content and no plasmids.


2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Naoko Moriya ◽  
Kazuma Nakano ◽  
Akino Shiroma ◽  
Misuzu Shinzato ◽  
Noriko Ashimine ◽  
...  

ABSTRACT Lactobacillus plantarum LQ80 is a strain isolated from liquid feed for pigs. We determined the complete genome sequence of this strain using the PacBio RS II platform. LQ80 contained a single circular chromosome of 3,230,192 bp, with 44.66% G+C content and seven plasmids.


2017 ◽  
Vol 5 (18) ◽  
Author(s):  
Qinghai Hu ◽  
Jingjing Qi ◽  
Huimin Bo ◽  
Guangqing Liu ◽  
Minjie Tao ◽  
...  

ABSTRACT The complete genome sequence of highly virulent Riemerella anatipestifer strain HXb2 was determined. The genome consisted of a single circular chromosome of 2,425,237 bp containing 2,383 putative open reading frames (ORFs), 9 rRNA operons, and 40 tRNA genes.


2020 ◽  
Vol 9 (16) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Erina Hase ◽  
Tomomasa Maruya

We isolated Pseudomonas otitidis strain MrB4 from the near-shore area of Lake Biwa in Japan and generated its complete genome sequence. MrB4 possesses a single circular chromosome of 6,089,454 bp, with ∼97% average nucleotide identity to the P. otitidis type strain MCC10330 (draft genome).


2019 ◽  
Vol 8 (47) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Kouki Izumi

A novel strain of Alteromonas, I4, was isolated from a shallow beach of the Japan Sea. Here, we report the complete genome sequence of I4; this strain contains a single circular chromosome (5,133,645 bp; G+C content, 48.4%) and a single circular putative plasmid (123,836 bp; G+C content, 45.1%).


2021 ◽  
Vol 10 (26) ◽  
Author(s):  
Lin Lin ◽  
Chunhui Li ◽  
Fei Wang ◽  
Xueying Wang ◽  
Yue Zhang ◽  
...  

Here, we report the complete genome sequence of clinical Pasteurella multocida strain HuN001, which was cultured from a sputum sample from a patient with pneumonia. Oxford Nanopore Technologies sequencing provided a complete genome sequence of P. multocida HuN001, which contains a 2,287,216-bp circular chromosome with an average G+C content of 40.33%.


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