scholarly journals PTU-099 Discovery of Potential Plasma Biomarkers of Cholangiocarcinoma Utilising Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (SELDI-TOF MS)

Gut ◽  
2013 ◽  
Vol 62 (Suppl 1) ◽  
pp. A86.1-A86
Author(s):  
C A Wadsworth ◽  
V M Horneffer-van der Sluis ◽  
A Zabron ◽  
S D Taylor-Robinson ◽  
R J Edwards ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Plasma Biomarkers ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced ◽  
Tof Ms

Investigation of Tissue Inhibitor of Metalloproteinases 1 in Plasma from Colorectal Cancer Patients and Blood Donors by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2007 ◽  
Vol 22 (2) ◽  
pp. 89-94 ◽  
Author(s):  
M.B. Caspersen ◽  
N.M. Sørensen ◽  
A.S. Schrohl ◽  
P. Iversen ◽  
H.J. Nielsen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Laser Desorption ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced ◽  
Tof Ms

Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and <0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.

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