scholarly journals Polymerase chain reaction for diagnosis of genital herpes in a genitourinary medicine clinic

2002 ◽  
Vol 78 (1) ◽  
pp. 21-25 ◽  
Author(s):  
A Scoular
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Chain Reaction ◽  
Medicine Clinic ◽  
Polymerase Chain ◽  
Genitourinary Medicine

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

Polymerase chain reaction for diagnosis of genital herpes: a missed opportunity?

2005 ◽  
Vol 16 (8) ◽  
pp. 579-581 ◽  
Author(s):  
E Agius ◽  
G Arthur ◽  
K Mandhyan ◽  
D Mercey
Keyword(s):  
Polymerase Chain Reaction ◽  
Tissue Culture ◽  
Herpes Simplex ◽  
Genital Herpes ◽  
Chain Reaction ◽  
Recurrent Genital Herpes ◽  
Delay In Diagnosis ◽  
Polymerase Chain ◽  
Simplex Virus ◽  
Missed Opportunity

This study audited the utilization of herpes simplex virus polymerase chain reaction (HSV PCR) in the investigation of recurrent anogenital ulceration at the Mortimer Market Centre. Clinic guidelines for use of HSV PCR were modified in April 2003 to expand PCR use. Ninety-six case-notes belonging to patients presenting with recurrent anogenital ulceration between 1 April and 16 October 2003 were reviewed and 59 were suitable for inclusion. Details of the investigations carried out at each visit were recorded. HSV PCR was used according to guidelines in eight of the 59 cases studied. This audit showed under-utilization of HSV PCR testing with poor adherence to clinic guidelines when cases of suspected recurrent genital herpes were investigated. This led to under-diagnosis and delay in diagnosis. This audit stresses the importance of informing all clinical staff of the improved sensitivity and relative affordability of HSV PCR compared with HSV tissue culture.

Diagnosis of Genital Herpes by Polymerase Chain Reaction Amplification

1993 ◽  
Vol 37 (12) ◽  
pp. 987-990 ◽  
Author(s):  
Tetsuya Goto ◽  
Yuki Yamaguchi ◽  
Madoka Hashido ◽  
Hiroyuki Yoshikawa ◽  
Takashi Kawana
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Reaction Amplification ◽  
Polymerase Chain

scholarly journals Role of polymerase chain reaction in detection of genital herpes

2013 ◽  
Vol 79 (5) ◽  
pp. 705 ◽  
Author(s):  
RadhaKanta Ratho ◽  
AJ Kanwar ◽  
Baijayantimala Mishra ◽  
MiniP Singh ◽  
Kapil Goyal
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Chain Reaction ◽  
Polymerase Chain

Audit of treatment and contact-tracing rates in immediate (presumptive) versus delayed (polymerase chain reaction) diagnosis of chlamydial infection

2005 ◽  
Vol 16 (7) ◽  
pp. 502-503
Author(s):  
I Fernando ◽  
D J Clutterbuck
Keyword(s):  
Polymerase Chain Reaction ◽  
Success Rate ◽  
Chlamydial Infection ◽  
Contact Tracing ◽  
Chain Reaction ◽  
Medicine Clinic ◽  
Gonococcal Urethritis ◽  
Polymerase Chain

An audit of all cases of chlamydial infection diagnosed in men at the Edinburgh genitourinary (GU) medicine clinic over a six-month period from January 2003 is reported. In all, 189 men identified as requiring treatment for possible chlamydial infection on first attendance (because of contact with a partner with chlamydia or the diagnosis of non-gonococcal urethritis [NGU] on microscopy), who later proved chlamydia-positive by polymerase chain reaction (PCR), were compared with 83 men in whom infection was identified only on receipt of a PCR result. Treatment rates were 100% in the first group and 97.6% in the second group (χ2 0.046, P <0.05). In men presumptively diagnosed and treated, 88.6% of contacts identified were confirmed as traced, compared with 90% confirmed as traced in the group diagnosed by PCR alone. Our audit suggests that identifying men with chlamydial NGU by routine microscopy may carry a small but significant advantage in increasing treatment rates, but makes no difference to contact-tracing success rate.

Frequent Detection of Genital Herpes Simplex Virus DNA by Polymerase Chain Reaction Among Pregnant Women

1995 ◽  
Vol 50 (2) ◽  
pp. 89-91
Author(s):  
Richard W. Cone ◽  
Ann C. Hobson ◽  
Zane Brown ◽  
Rhoda Ashley ◽  
Sylvia Berry ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Pregnant Women ◽  
Genital Herpes ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus
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