scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

Polymerase chain reaction for diagnosis of genital herpes: a missed opportunity?

2005 ◽  
Vol 16 (8) ◽  
pp. 579-581 ◽  
Author(s):  
E Agius ◽  
G Arthur ◽  
K Mandhyan ◽  
D Mercey
Keyword(s):  
Polymerase Chain Reaction ◽  
Tissue Culture ◽  
Herpes Simplex ◽  
Genital Herpes ◽  
Chain Reaction ◽  
Recurrent Genital Herpes ◽  
Delay In Diagnosis ◽  
Polymerase Chain ◽  
Simplex Virus ◽  
Missed Opportunity

This study audited the utilization of herpes simplex virus polymerase chain reaction (HSV PCR) in the investigation of recurrent anogenital ulceration at the Mortimer Market Centre. Clinic guidelines for use of HSV PCR were modified in April 2003 to expand PCR use. Ninety-six case-notes belonging to patients presenting with recurrent anogenital ulceration between 1 April and 16 October 2003 were reviewed and 59 were suitable for inclusion. Details of the investigations carried out at each visit were recorded. HSV PCR was used according to guidelines in eight of the 59 cases studied. This audit showed under-utilization of HSV PCR testing with poor adherence to clinic guidelines when cases of suspected recurrent genital herpes were investigated. This led to under-diagnosis and delay in diagnosis. This audit stresses the importance of informing all clinical staff of the improved sensitivity and relative affordability of HSV PCR compared with HSV tissue culture.

scholarly journals Comparison of the Membrane-Filtration Fluorescent Antibody Test, the Enzyme-Linked Immunosorbent Assay, and the Polymerase Chain Reaction to Detect Renibacterium Salmoninarum in Salmonid Ovarian Fluid

1998 ◽  
Vol 10 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Ronald J. Pascho ◽  
Dorothy Chase ◽  
Connie L. McKibben
Keyword(s):  
Polymerase Chain Reaction ◽  
Immunosorbent Assay ◽  
Membrane Filtration ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Fluid ◽  
Chain Reaction ◽  
Renibacterium Salmoninarum ◽  
Polymerase Chain

Ovarian fluid samples from naturally infected chinook salmon ( Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels of R. salmoninarum cells. Among the positive fish, the R. salmoninarum concentrations ranged from 25 cells/ml to 4.3 × 109 cells/ml. A soluble antigenic fraction of R. salmoninarum was detected in 39% of the fish (40/103) by the ELISA. The ELISA is considered one of the most sensitive detection methods for bacterial kidney disease in tissues, yet it did not detect R. salmoninarum antigen consistently at bacterial cell concentrations below about 1.3 × 104 cells/ml according to the MF-FAT counts. When total DNA was extracted and tested in a nested PCR designed to amplify a 320-base-pair region of the gene encoding a soluble 57-kD protein of R. salmoninarum, 100% of the 100 samples tested were positive. The results provided strong evidence that R. salmoninarum may be present in ovarian fluids thought to be free of the bacterium on the basis of standard diagnostic methods.

Sign in / Sign up

Export Citation Format

Share Document