Experimental infection of lambs with Border disease virus isolated from a Pyrenean chamois

2010 ◽  
Vol 167 (16) ◽  
pp. 619-621 ◽  
Author(s):  
O. Cabezon ◽  
R. Velarde ◽  
R. Rosell ◽  
S. Lavin ◽  
J. Segales ◽  
...  
Keyword(s):  
Disease Virus ◽  
Experimental Infection ◽  
Border Disease Virus ◽  
Border Disease ◽  
Pyrenean Chamois

scholarly journals EXPERIMENTAL INFECTION OF PREGNANT PYRENEAN CHAMOIS (RUPICAPRA PYRENAICA) WITH BORDER DISEASE VIRUS SUBTYPE 4

2013 ◽  
Vol 49 (1) ◽  
pp. 55-68 ◽  
Author(s):  
Claire Martin ◽  
Véronique Duquesne ◽  
Jean-Michel Guibert ◽  
Coralie Pulido ◽  
Emmanuelle Gilot-Fromont ◽  
...  
Keyword(s):  
Disease Virus ◽  
Experimental Infection ◽  
Border Disease Virus ◽  
Rupicapra Pyrenaica ◽  
Border Disease ◽  
Pyrenean Chamois ◽  
Virus Subtype

scholarly journals Experimental Infection of Pigs with Border Disease Virus Isolated from Pyrenean Chamois (Rupicapra Pyrenaica)

2010 ◽  
Vol 22 (3) ◽  
pp. 360-365 ◽  
Author(s):  
Cabezón Oscar ◽  
Rosa Rosell ◽  
Marina Sibila ◽  
Lavín Santiago ◽  
Ignasi Marco ◽  
...  
Keyword(s):  
Disease Virus ◽  
Experimental Infection ◽  
Border Disease Virus ◽  
Rupicapra Pyrenaica ◽  
Border Disease ◽  
Pyrenean Chamois

scholarly journals Detection of Border Disease Virus in Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

2009 ◽  
Vol 21 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Ana L. García-Pérez ◽  
Esmeralda Minguijón ◽  
Jesús F. Barandika ◽  
Gorka Aduriz ◽  
Inés Povedano ◽  
...  
Keyword(s):  
Disease Virus ◽  
Experimental Infection ◽  
Clinical Symptoms ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Rt Pcr ◽  
Genotype 4 ◽  
Antigen Elisa ◽  
Border Disease

The purpose of the present study was to evaluate the use of enzyme-linked immunosorbent assay (ELISA) antigen detection in blood or fetal fluids and reverse transcription polymerase chain reaction (RT-PCR) amplification in tissues for routine laboratory diagnosis of Border disease virus (BDV) infection. Samples from 67 fetuses, 6 stillbirths, and 11 lambs from 25 commercial flocks with suspicion of BDV abortion and 3 fetuses, 7 stillbirths, and 15 lambs obtained from an experimental infection with a local isolate (BDV genotype 4) were investigated. Presence of BDV was detected by RT-PCR in 7.9% of fetuses, 50% of stillbirths, and 50% of lambs from the commercial flocks analyzed, corresponding to 8 of the 25 farms (32%). A similar percentage of the lambs and stillbirths from the experimental infection were positive by RT-PCR of tissue samples (54.5%), and the highest positivity was detected in lymph node, thyroid gland, and kidney. The current study revealed that RT-PCR analysis of stillbirths and lambs with clinical symptoms is more suitable than the analysis of fetuses to confirm the presence of BDV in a flock. Pestiviral antigen was detected by antigen ELISA in a high proportion of fetuses (24/58) and stillbirths (3/4) from commercial flocks, but in lambs, the presence of colostral antibodies masked the detection of the antigen by ELISA. Nevertheless, in lambs from the experimental infection that were not fed colostrum, antigen ELISA was less efficient than RT-PCR in detecting viral presence in stillbirths and lambs. Antigen ELISA is therefore recommended for fetuses with advanced autolysis that can adversely affect RNA integrity.

scholarly journals Experimental infection with chamois border disease virus causes long-lasting viraemia and disease in Pyrenean chamois (Rupicapra pyrenaica)

2011 ◽  
Vol 92 (11) ◽  
pp. 2494-2501 ◽  
Author(s):  
Oscar Cabezón ◽  
Roser Velarde ◽  
Gregorio Mentaberre ◽  
Laura Fernández-Sirera ◽  
Encarna Casas-Díaz ◽  
...  
Keyword(s):  
Disease Virus ◽  
Neutralizing Antibodies ◽  
Border Disease Virus ◽  
Post Inoculation ◽  
Experimental Conditions ◽  
Humoral Immune ◽  
Rupicapra Pyrenaica ◽  
Viral Virulence ◽  
Border Disease ◽  
Pyrenean Chamois

Since 2001, severe outbreaks of disease associated with border disease virus (BDV) infection have been reported in Pyrenean chamois. The disease is characterized by variable degrees of cachexia, alopecia and neurological manifestations prior to death. The aim of this study was to investigate this disease under experimental conditions. To assess viral virulence, humoral immune response, dissemination and probable routes of transmission, seven chamois (five seronegative and two seropositive for BDV) were inoculated with a BDV isolated from a naturally infected chamois. A group of three chamois were maintained as uninfected controls. The five seronegative chamois became viraemic from day 2 post-inoculation (p.i.) until their death (three animals) or the end of the experiment (on day 34 p.i.) and developed neutralizing antibodies from day 18 p.i. until the end of the study. Continuous shedding of the virus was detected by RT-PCR in oral, nasal and rectal swabs in viraemic chamois from day 5 p.i. Despite none of the viraemic chamois showing obvious neurological signs, all of them had a non-suppurative meningoencephalitis as seen in naturally infected chamois. The two inoculated BDV-seropositive chamois did not become viraemic. This study confirms that BDV is the primary agent of the disease that has been affecting chamois populations in recent years in the Pyrenees and that previously acquired humoral immunity is protective.

Complete genomic sequence of a border disease virus isolated from Pyrenean chamois

2010 ◽  
Vol 152 (1-2) ◽  
pp. 164-168 ◽  
Author(s):  
Stefan Vilcek ◽  
Kim Willoughby ◽  
Peter Nettleton ◽  
Paul Becher
Keyword(s):  
Disease Virus ◽  
Genomic Sequence ◽  
Border Disease Virus ◽  
Complete Genomic Sequence ◽  
Border Disease ◽  
Pyrenean Chamois ◽  
Complete Genomic

scholarly journals Spatial and Temporal Phylogeny of Border Disease Virus in Pyrenean Chamois (Rupicapra p. pyrenaica)

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168232 ◽  
Author(s):  
Camilla Luzzago ◽  
Erika Ebranati ◽  
Oscar Cabezón ◽  
Laura Fernández-Sirera ◽  
Santiago Lavín ◽  
...  
Keyword(s):  
Disease Virus ◽  
Border Disease Virus ◽  
Border Disease ◽  
Pyrenean Chamois

Morphometric analysis of growth retardation in fetal lambs following experimental infection of pregnant ewes with Border Disease virus

1997 ◽  
Vol 62 (3) ◽  
pp. 245-248 ◽  
Author(s):  
J.F Caffrey ◽  
A.M Dudgeon ◽  
W.J.C Donnelly ◽  
B.J Sheahan ◽  
G.J Atkins
Keyword(s):  
Disease Virus ◽  
Morphometric Analysis ◽  
Experimental Infection ◽  
Growth Retardation ◽  
Border Disease Virus ◽  
Border Disease

scholarly journals Border Disease Virus Shedding and Detection in Naturally Infected Pyrenean Chamois (Rupicapra Pyrenaica)

2010 ◽  
Vol 22 (5) ◽  
pp. 744-747 ◽  
Author(s):  
Oscar Cabezón ◽  
Rosa Rosell ◽  
Roser Velarde ◽  
Gregorio Mentaberre ◽  
Encarna Casas-Díaz ◽  
...  
Keyword(s):  
Disease Virus ◽  
Border Disease Virus ◽  
Virus Shedding ◽  
Rupicapra Pyrenaica ◽  
Border Disease ◽  
Pyrenean Chamois

Haematology and serum chemistry of Pyrenean chamois (Rupicapra pyrenaica) naturally infected with a border disease virus

2011 ◽  
Vol 90 (3) ◽  
pp. 463-467 ◽  
Author(s):  
L. Fernández-Sirera ◽  
G. Mentaberre ◽  
J.R. López-Olvera ◽  
R. Cuenca ◽  
S. Lavín ◽  
...  
Keyword(s):  
Disease Virus ◽  
Border Disease Virus ◽  
Serum Chemistry ◽  
Rupicapra Pyrenaica ◽  
Border Disease ◽  
Pyrenean Chamois
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