Plantlet formation from Lycopersicon esculentum leaf callus

1974 ◽  
Vol 52 (6) ◽  
pp. 1429-1432 ◽  
Author(s):  
Vasantha Padmanabhan ◽  
E. F. Paddock ◽  
W. R. Sharp
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Shoot Formation ◽  
Growth Hormones ◽  
Root Initiation ◽  
Plantlet Formation ◽  
Callus Proliferation ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Indole 3 Acetic Acid

Explants were obtained from leaves of three strains of tomato and grown on a modified Murashige and Skoog's medium with various combinations of indole-3-acetic acid (IAA) and kinetin. Callus proliferation began by 8–10 days. Root initiation was very common, particularly at 2 mg/liter IAA and 2 mg/liter kinetin. Shoot formation occurred within 30 days but only at a specific combination of concentrations of the two growth hormones (4 mg/liter IAA + 4 mg/liter kinetin). Most shoots became plantlets by 10 days after transfer to basal Murashige and Skoog's medium. Shoot-forming potential was neither correlated with callus-forming potential nor with vigor of strain.

Conditions d'apparition d'une embryogénèse somatique sur des cals issus de la culture de tissus foliaires du chêne vert (Quercus ilex)

1989 ◽  
Vol 67 (4) ◽  
pp. 1066-1070 ◽  
Author(s):  
C. Féraud-Keller ◽  
H. Espagnac
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Quercus Ilex ◽  
Foliar Tissue ◽  
Murashige And Skoog's Medium ◽  
Old Trees ◽  
Murashige And Skoog’S Medium

The proliferation of parenchymatous foliar tissue of Quercus ilex was obtained from current year leaves taken from old trees and cultivated in vitro on modified Murashige and Skoog's medium supplemented with benzylaminopurine (4 mg ∙ L−1) and naphthyl acetic acid (0.5 mg ∙ L−1). Only the fragments cultured in October reacted. The neoformations only appeared on calluses that had not been subcultured for 7 months. Primary nodules arising on these calluses were removed and subcultured on the same medium either in the dark or in the light. In the dark only, they produced secondary nodules, which were the source of somatic embryos both in light and dark. Presently, they seem to regulate their structure in the dark but they do not develop in a way that leads to germination.

scholarly journals Indole-3-Acetic Acid Metabolism in Relation to Root Initiation

1985 ◽  
Author(s):  
Jerry D. Cohen ◽  
Ephraim Epstein ◽  
Mark Roh ◽  
Joseph Riov
Keyword(s):  
Acetic Acid ◽  
Acid Metabolism ◽  
Root Initiation ◽  
Indole 3 Acetic Acid

Changes in indole-3-acetic acid levels during tomato (Lycopersicon esculentum Mill.) seed development

1992 ◽  
Vol 11-11 (5-6) ◽  
Author(s):  
Val�rie Hocher ◽  
Bruno Sotta ◽  
R�gis Maldiney ◽  
Magda Bonnet ◽  
Emile Miginiac
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Seed Development ◽  
Lycopersicon Esculentum Mill ◽  
Indole 3 Acetic Acid

Dynamics of Endogenous Indole-3-acetic Acid and Cytokinins During Adventitious Shoot Formation in Ipecac

2017 ◽  
Vol 36 (4) ◽  
pp. 805-813 ◽  
Author(s):  
Imari Koike ◽  
Koudai Taniguchi ◽  
Koichiro Shimomura ◽  
Mikihisa Umehara
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation ◽  
Indole 3 Acetic Acid

scholarly journals In vitro Tomato Fruit Cultures Demonstrate a Role for Indole-3-acetic Acid in Regulating Fruit Ripening

1996 ◽  
Vol 121 (3) ◽  
pp. 520-524 ◽  
Author(s):  
Jerry D. Cohen
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Fruit Ripening ◽  
Culture Media ◽  
Tomato Fruit ◽  
In Vitro System ◽  
Auxin Conjugates ◽  
Time Period ◽  
Indole 3 Acetic Acid

An in vitro system was used for the production of tomato (Lycopersicon esculentum) fruit in culture starting from immature flowers. This system produced small parthenocarpic (seedless) fruit in response to 10-4m indole-3-acetic acid (IAA) supplied in the medium. Other auxins, auxin conjugates and antiauxins tested were not effective or produced markedly fewer fruit. Additional IAA supplied to the fruit culture media before breaker stage resulted in an increase in the time period between breaker and red-ripe stages from 7 days without additional IAA to 12 days when 10-5m IAA was added. These results suggest that significant changes in the ripening period could be obtained by alteration of auxin relationships in tomato fruit.

scholarly journals Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 9-14 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
J. Faragó ◽  
Z. Jureková
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Shoot Regeneration ◽  
Explant Type ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Lycopersicon Esculentum Mill ◽  
Shoot Primordia ◽  
Indole 3 Acetic Acid

The regeneration capacity of six types of explants (segments from hypocotyl, cotyledons, epicotyl, leaf, internodes and petiole) was compared in 13 cultivars of tomato (Lycopersicon esculentum Mill). Explants were cultured on a regeneration medium containing 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. The number of shoot primordia and shoots with 1 or more fully developed leaves was evaluated after 6 weeks. The regeneration capacity was significantly influenced by cultivars and explant types. The total number of shoot primordia produced in all types of explants was highest in the cultivars Hana and Premium and lowest in UC 82 and Money Marker. Cv. Hana also produced the highest number of shoots. The most responsive explants in most cultivars were hypocotyls and epicotyls with up to 100% regeneration and mean production of 6.3 and 6.5 shoot primordia per explant, respectively.  

scholarly journals Quantification of Indole-3-Acetic Acid in Dark-Grown Seedlings of the Diageotropica and Epinastic Mutants of Tomato (Lycopersicon esculentum Mill.)

1988 ◽  
Vol 88 (3) ◽  
pp. 780-784 ◽  
Author(s):  
David W. Fujino ◽  
Scott J. Nissen ◽  
A. Daniel Jones ◽  
David W. Burger ◽  
Kent J. Bradford
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Lycopersicon Esculentum Mill ◽  
Indole 3 Acetic Acid

scholarly journals Genotypic Differences in Shoot-forming Capacity of Cultured Leaf Explants of Lycopersicon hirsutum

HortScience ◽  
1991 ◽  
Vol 26 (10) ◽  
pp. 1317-1320 ◽  
Author(s):  
John R. Stommel ◽  
Stephen L. Sinden
Keyword(s):  
Acetic Acid ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Genotypic Differences ◽  
Lycopersicon Hirsutum ◽  
Shoot Induction ◽  
Wild Tomato Species ◽  
Cotyledon Explants ◽  
Tomato Species ◽  
Indole 3 Acetic Acid

Cultured leaf explants obtained from 36 accessions of the wild tomato species, Lycopersicon hirsutum Humb. and Bonpl., were evaluated for morphogenic capacity in response to three cytokinins (zeatin, BA, and kinetin) in combination with IAA. Media containing 0.1 μm IAA plus 4.6 or 9.2 μm zeatin were optimal for shoot induction. Cotyledon explants were superior to true leaf explants for obtaining shoot formation. Morphogenic responses of L. hirsutum f. typicum and L. hirsutum f. glabratum were clearly accession-dependent and ranged from exceptional with numerous shoots produced to recalcitrant with no shoots produced. The high morphogenetic capacity of leaf explants from L. hirsutum f. typicum accession 128644 was also evident in protoplast-derived calli that readily regenerated shoots. Chemical names used (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin), N-(phenylmethyl) -1H-purin-6-amine (BA), N- (2-furanylmethyl) -1H- purin-6-amine (kinetin), 1H- indole-3-acetic acid (IAA).

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