Changes in the peroxidase activity of subcellular fractions from aging Phaseolus hypocotyls

1975 ◽  
Vol 53 (9) ◽  
pp. 852-860 ◽  
Author(s):  
B. Truelove ◽  
R. Rodriguez-Kabana ◽  
Larry R. Jones
Keyword(s):  
Phaseolus Vulgaris ◽  
Peroxidase Activity ◽  
Specific Activity ◽  
Total Activity ◽  
Chronological Age ◽  
Phaseolus Vulgaris L ◽  
Soluble Peroxidase ◽  
Tissue Homogenates ◽  
Hypocotyl Tissue ◽  
Nitrogen Contents

Changes in nitrogen contents and peroxidase activities of fractions isolated from hypocotyl tissue of Black Valentine bean (Phaseolus vulgaris L.) of increasing age were studied. As beans aged in darkness, a decreasing percentage of their nitrogen content was recovered in the isolated particulate fractions. Peroxidase activity of particulate fractions from dark-grown beans accounted for 49% of the total activity of both 3-day-old seedlings and 16-day-old senescing plants. Peroxidase specific activity of dark-grown tissue homogenates did not increase with plant age; however, after a certain period of growth, further aging resulted in increased peroxidase specific activity associated with the particulate fractions. Between day 3 and day 8 the patterns of peroxidase activity of the different fractions varied, but over the period day 9 to day 16, the patterns of all fractions were correlated. The nitrogen contents and peroxidase activities of fractions isolated from beans transferred from dark to light were different from those of fractions from beans of similar chronological age kept in darkness. Transfer of plants to light resulted in increased soluble peroxidase activity and prevention of the steep increase in particulate fraction activity recorded for dark-grown plants.

scholarly journals Partial purification and some properties of a cholinesterase from bush bean (Phaseolus vulgaris L.) roots

1978 ◽  
Vol 175 (3) ◽  
pp. 769-777 ◽  
Author(s):  
D H Mansfield ◽  
G Webb ◽  
D G Clark ◽  
I E P Taylor
Keyword(s):  
Phaseolus Vulgaris ◽  
Specific Activity ◽  
Apparent Molecular Weight ◽  
Fold Increase ◽  
Partial Purification ◽  
Phaseolus Vulgaris L ◽  
Native Form ◽  
Bush Bean ◽  
Electric Eel ◽  
Stokes Radius

A cholinesterase was partially purified from bush bean (Phaseolus vulgaris L.) roots by using acridinium-based ligand affinity chromatography. The procedure gave a 78-fold increase in specific activity, although at least three inactive contaminants remained. The enzyme activity was maximal against acetyl esters of choline and was inhibited by neostigmine. Di-isopropyl phosphorofluoridate completely inhibited activity at concentrations greater than 0.1 mM. The catalytic centre activity was 2 × 10(-4) times that of electric eel acetylcholinesterase. Cholinesterase activity appeared as a peak (s = 4.2 +/- 0.1 S) after isokinetic sedimentation. The Stokes radius was 4.00 nm and the apparent molecular weight was 72700 +/- 1900. The smallest active and native form of the enzyme appeared to be a monomer. This contrasts with animal acetylcholinesterases, in which the smallest active and native forms are multimeric.

Tissue and subcellular immunolocalisation of enzymes of lignin synthesis in differentiating and wounded hypocotyl tissue of French bean (Phaseolus vulgaris L.)

Planta ◽  
1994 ◽  
Vol 192 (2) ◽  
Author(s):  
ColinG. Smith ◽  
MatthewW. Rodgers ◽  
Alfred Zimmerlin ◽  
Dudley Ferdinando ◽  
G.Paul Bolwell
Keyword(s):  
Phaseolus Vulgaris ◽  
French Bean ◽  
Phaseolus Vulgaris L ◽  
Lignin Synthesis ◽  
Hypocotyl Tissue

Tissue and subcellular immunolocalisation of enzymes of lignin synthesis in differentiating and wounded hypocotyl tissue of French bean (Phaseolus vulgaris L.)

Planta ◽  
1994 ◽  
Vol 192 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Colin G. Smith ◽  
Matthew W. Rodgers ◽  
Alfred Zimmerlin ◽  
Dudley Ferdinando ◽  
G. Paul Bolwell
Keyword(s):  
Phaseolus Vulgaris ◽  
French Bean ◽  
Phaseolus Vulgaris L ◽  
Lignin Synthesis ◽  
Hypocotyl Tissue

ĐIỀU TRA, TINH SẠCH VÀ TÌM HIỂU TÍNH CHẤT ĐẶC TRƯNG CỦA LECTIN MỘT SỐ GIỐNG ĐẬU CÔ VE (PHASEOLUS VULGARIS L.)

2013 ◽  
Vol 75 (6) ◽  
Author(s):  
Cao Đăng Nguyên ◽  
Nguyễn Thị Cẩm Hạnh
Keyword(s):  
Phaseolus Vulgaris ◽  
Phaseolus Vulgaris L ◽  
White Bean

Đã điều tra lectin của 6 giống đậu cô ve thấy rằng cả 6 giống đều có hoạt tính lectin trong đó giống đậu cove hạt trắng dạng bụi (white bean core bush type white seeds) có hoạt tính lectin mạnh nhất, đặc biệt đối với hồng cầu trâu, bò, lợn. Lectin của 6 giống này đều không có biểu hiện đặc hiệu nhóm máu. Lectin đậu cove hạt trắng dạng bụi hoạt động tốt nhất ở nhiệt độ 300C – 400C, pH 6,8 – 7,6. Các đường α-D-glucose, α-D-galactose, D-mannose, D-fructose, D-saccharide,  D-lactose, D-arabinose và D-manitose ở nồng độ 0,05 – 0,1 M có tác dụng kìm hãm hoạt tính của lectin đậu cove hạt trắng dạng bụi. Lectin này cũng bị kìm hãm bởi protein của một số huyết thanh người và động vật (trâu, bò, lợn). Đã tinh sạch lectin đậu cove hạt trắng dạng bụi có độ tinh sạch gấp khoảng 52 lần so với dịch thô ban đầu. Trên gel polyacrylamide thấy xuất hiện 5 band có khối lượng phân tử trong khoảng 30 – 97 kDa.

Análisis de la variabilidad genética en poblaciones primitivas y silvestres de Phaseolus vulgaris, mediante marcadores bioquímicos y moleculares

2006 ◽  
Author(s):  
◽  
Marta Zulema Galván
Keyword(s):  
Phaseolus Vulgaris ◽  
Phaseolus Vulgaris L

El poroto común (Phaseolus vulgaris L.) es una leguminosa originaria de América y representa un componente importante en la dieta de la población latinoamericana por su alto contenido en proteínas y carbohidratos. El poroto cultivado se originó de los porotos silvestres, que son plantas anuales, herbáceas y trepadoras, que se distribuyen desde el norte de México hasta el noroeste de la Argentina. Estudios basados en caracteres morfológicos, bioquímicos y moleculares revelaron que tanto dentro de las variedades cultivadas como de las silvestres existen dos acervos génicos principales, uno Andino y el otro Mesoamericano. Las provincias del Noroeste Argentino (NOA) albergan un gran número de porotos silvestres y de variedades locales tradicionales (primitivas o “landraces”) mantenidas durante años en un sistema de cultivo tradicional. Sin embargo muchas de estas poblaciones están en peligro de extinción debido a las presiones de explotación forestal y pastoreo existentes en la zona, por lo que resulta de fundamental importancia su recolección y estudio. En esta tesis se analizó la variabilidad genética de un grupo de poblaciones silvestres y primitivas de poroto común del NOA, recolectadas en diferentes sitios de las provincias fitogeográficas de las Yungas, Prepuna y del Chaco, entre los 1300 y 2900 msnm, utilizando marcadores bioquímicos (proteínas de reserva de las semillas: faseolinas) y moleculares (RAPD e ISSR).

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