Restriction enzyme mapping of the nuclear ribosomal cistron in selected Laminariales (Phaeophyta): a phylogenetic assessment

Restriction enzyme mapping of the nuclear ribosomal cistron was completed for a variety of Laminariales. Taxa investigated included Alaria marginata Postels and Ruprecht, Egregia menziesii (Turner) Areschoug, Eisenia arborea Areschoug, Lessoniopsis littoralis (Tilden) Reinke, Macrocystis integrifolia Bory, Nereocystis leutkeana (Mertens) Postels and Ruprecht, Postelsia palmaeformis Ruprecht, and Pterygophora californica Ruprecht, with Sargassum muticum (Yendo) Fensholt (Fucales) as an outgroup. The restriction maps establish a foundation for future phylogenetic, as well as other molecular, investigations in the kelp. We also assess restriction enzyme mapping of the nuclear ribosomal cistron for suitability in resolving intrafamilial and interfamilial taxonomic relationships in the Laminariales. The intergenic spacer proved too variable to be of use for phylogenetic comparisons at this level. Conversely, the gene regions were highly conserved, with only three restriction-site differences observed among all the laminarialean taxa investigated. Key words: Laminariales, rRNA, restriction enzyme mapping.

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Restriction Enzyme mapping: A simple student practical

Biochemical Education ◽

10.1016/0307-4412(90)90221-9 ◽

1990 ◽

Vol 18 (3) ◽

pp. 144-146 ◽

Cited By ~ 2

Author(s):

Stephen J Higgins ◽

B David Hames ◽

Elizabeth McIntosh ◽

Alan Colman

Keyword(s):

Restriction Enzyme ◽

Restriction Enzyme Mapping ◽

Enzyme Mapping

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Identification of restriction enzyme in the FSHR gene of indonesian local cattle

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012024 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012024

Author(s):

P W Prihandini ◽

A Primasari ◽

M Luthfi ◽

D Pamungkas ◽

A P Z N L Sari ◽

...

Keyword(s):

Restriction Enzyme ◽

Restriction Site ◽

Follicle Stimulating Hormone ◽

Restriction Enzymes ◽

Future Studies ◽

Fshr Gene ◽

Pcr Rflp ◽

Mapping Analysis ◽

Pcr Products ◽

Enzyme Mapping

Abstract The restriction enzyme is important for genotyping using the PCR-RFLP technique. Therefore, this study aims to identify the restriction enzyme mapping in the partial sequence of the follicle-stimulating hormone receptor (FSHR) gene in Indonesian local cattle. A total of 29 samples sized 306 bp, were aligned with Genbank sequence acc no. NC_032660, resulting three polymorphic sites, namely g.193G>C, g.227T>C, and g.275A>C. Furthermore, the restriction mapping analysis using the NEBcutter program V2.0 showed that no enzyme recognized the SNP g.275A>C, while the SNP g.193G>C and g.227T>C were identified by the AluI and MscI enzymes, respectively. The AluI enzyme cuts at two positions (193 bp and 243 bp) in the G allele sample producing three fragments namely 50 bp, 63 bp, and 193 bp, meanwhile, in the C allele, the AluI cuts only in position 243 bp, hence, the fragment products are 63 bp and 243 bp. In contrast, the MscI enzyme was only recognized in the T allele, producing fragments sized 77 bp and 229 bp but failed to identify the restriction site along with the PCR products in the C allele. Based on the results, the SNPs (g.193G>C and g.227T>C) and restriction enzymes (AluI and MscI) are applicable for genotyping local Indonesian cattle using the PCR-RFLP technique in future studies.

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