Molecules and morphology in basidiomycete phylogeny

1995 ◽  
Vol 73 (S1) ◽  
pp. 684-692 ◽  
Author(s):  
D. J. McLaughlin ◽  
M. E. Berres ◽  
L. J. Szabo

To obtain an understanding of the relationships of the basidiomycetes, especially those with horizontally partitioned metabasidia, and of the evolution of structural characters, members of nine orders and an additional four genera of simple-septate fungi (Auriculariales sensu lato) were studied using cladistic analysis of light microscopic and ultrastructural characters. Comparisons were made with the nucleotide sequence from the 5′ end of the nuclear large subunit ribosomal RNA gene analyzed with several algorithms, including parsimony and maximum likelihood. Analyses of both morphological and molecular characters support similar phylogenetic conclusions, but polarization of some morphological characters was difficult without guidance from molecular data. The Uredinales are shown to be an advanced taxon arising from the simple-septate Auriculariales sensu lato, and some characters that they share with the ascomycetes result from convergent evolution. The simple-septate Auriculariales consists of more than one clade, and the related gasteroid Pachnocybe ferruginea possesses numerous derived light microscopic characters, including holobasidia. Key words: basidiomycetes, character evolution, cladistics, molecular evolution, phylogeny.


Phytotaxa ◽  
2019 ◽  
Vol 387 (3) ◽  
pp. 210
Author(s):  
YA-XING WU ◽  
SHAN SHEN ◽  
CHANG-LIN ZHAO

A new wood-inhabiting fungal species, Podoscypha yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by annual, gregarious basidiocarps with spathulate to flabelliform pilei, a dimitic hyphal system with clamped generative hyphae, caulocystidia cylindrical with an apex, and ellipsoid to subglobose basidiospores measuring 3–3.5(–4) × 2.5–3(–3.5) µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of the nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS sequences showed that P. yunnanensis belonged to the genus Podoscypha and was closely related to P. fulvonitens and P. mellissii. Phylogeny based on ITS+nLSU sequences demonstrated that the new species formed a monophyletic lineage with a high support (100% BS, 100% BP, 1.00 BPP).





Phytotaxa ◽  
2017 ◽  
Vol 332 (1) ◽  
pp. 31 ◽  
Author(s):  
ZI-QIANG WU ◽  
SHAN SHEN ◽  
KAI-YUE LUO ◽  
ZHENG-HUI WANG ◽  
CHANG-LIN ZHAO

A new poroid wood-inhabiting fungal species, Atraporiella yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by cream pore surface when dry, which is easy to separate from substrate and very rapidly stained dark brown to black when bruised; hyphal system monomitic with generative hyphae hyaline to pale brown, thin-walled, unbranched, interwoven; slightly allantoid basidiospores, 2.2–3 × 0.8–1.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Atraporiella yunnanensis belonged to the residual polyporoid clade, formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and was closely related to A. neotropica, and then grouped with other related genera: Antrodiella, Pouzaroporia, Steccherinum and Xanthoporus. Both morphological and molecular characters confirmed the placement of the new species in Atraporiella.



1994 ◽  
Vol 3 (4) ◽  
pp. 219-228 ◽  
Author(s):  
R. E. Davis ◽  
T. J. Kelly ◽  
E. P. Masler ◽  
B. S. Thyagaraja ◽  
C. A. Rote ◽  
...  


Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).



Gene ◽  
1997 ◽  
Vol 194 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Ana Hurtado ◽  
Jonathan P. Clewley ◽  
Dennis Linton ◽  
Robert J. Owen ◽  
John Stanley


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