Overexpression of the putative extracytoplasmic function sigma (σ) factor FujE enhances FK506 production in Streptomyces sp. strain KCCM 11116P

The role of the putative extracytoplasmic function sigma (σ) factor FujE, which has not been characterized as a member of the FK506 biosynthetic gene cluster, on FK506 production was identified by gene deletion, overexpression, and transcription analysis experiments in Streptomyces sp. strain KCCM 11116P. Inactivation of fujE had no effect on FK506 production, growth, or morphological differentiation. Overexpression of fujE with integrative vectors increased FK506 production by 2.87-fold (24.5 ± 1.4 mg·L–1) compared with the wild type (8.5 ± 0.5 mg·L–1). Semiquantitative reverse transcription – polymerase chain reaction analysis indicated that the overexpression of fujE stimulates the transcription of the FK506 biosynthetic genes. These results demonstrated that fujE is a new member of the FK506 biosynthetic gene cluster.

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Faculty Opinions recommendation of Expression analysis of the tylosin-biosynthetic gene cluster: pivotal regulatory role of the tylQ product.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004877.55457 ◽

2002 ◽

Author(s):

Ben Shen

Keyword(s):

Gene Cluster ◽

Expression Analysis ◽

Biosynthetic Gene Cluster ◽

Regulatory Role ◽

Biosynthetic Gene

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Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

Bioscience Biotechnology and Biochemistry ◽

10.1093/bbb/zbaa082 ◽

2021 ◽

Vol 85 (3) ◽

pp. 714-721

Author(s):

Risa Takao ◽

Katsuyuki Sakai ◽

Hiroyuki Koshino ◽

Hiroyuki Osada ◽

Shunji Takahashi

Keyword(s):

Heterologous Expression ◽

Gene Cluster ◽

Secondary Metabolite ◽

Response Regulator ◽

Bac Library ◽

Gene Clusters ◽

Biosynthetic Gene Cluster ◽

Gene Organization ◽

Biosynthetic Gene ◽

Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

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Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412

Microbiology ◽

10.1099/mic.0.2006/003194-0 ◽

2007 ◽

Vol 153 (4) ◽

pp. 1095-1102 ◽

Cited By ~ 29

Author(s):

Si-Sun Choi ◽

Yoon-Ah Hur ◽

David H Sherman ◽

Eung-Soo Kim

Keyword(s):

T Cell ◽

Gene Cluster ◽

Biosynthetic Gene Cluster ◽

Biosynthetic Gene ◽

Streptomyces Sp

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Isolation and characterization of meridamycin biosynthetic gene cluster from Streptomyces sp. NRRL 30748

Gene ◽

10.1016/j.gene.2006.03.021 ◽

2006 ◽

Vol 377 ◽

pp. 109-118 ◽

Cited By ~ 16

Author(s):

Min He ◽

Bradley Haltli ◽

Mia Summers ◽

Xidong Feng ◽

John Hucul

Keyword(s):

Gene Cluster ◽

Biosynthetic Gene Cluster ◽

Biosynthetic Gene ◽

Streptomyces Sp ◽

Isolation And Characterization

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Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009

Organic Letters ◽

10.1021/acs.orglett.0c01224 ◽

2020 ◽

Vol 22 (12) ◽

pp. 4614-4619 ◽

Cited By ~ 2

Author(s):

Ling Liu ◽

Sainan Li ◽

Runze Sun ◽

Xiangjing Qin ◽

Jianhua Ju ◽

...

Keyword(s):

Gene Cluster ◽

Biosynthetic Gene Cluster ◽

Biosynthetic Gene ◽

Streptomyces Sp

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Gene Cluster Involved in the Biosynthesis of Griseobactin, a Catechol-Peptide Siderophore of Streptomyces sp. ATCC 700974

Journal of Bacteriology ◽

10.1128/jb.01250-09 ◽

2009 ◽

Vol 192 (2) ◽

pp. 426-435 ◽

Cited By ~ 46

Author(s):

Silke I. Patzer ◽

Volkmar Braun

Keyword(s):

Gene Cluster ◽

Linear Form ◽

Streptomyces Griseus ◽

Biosynthetic Gene Cluster ◽

Peptide Structure ◽

Biosynthetic Gene ◽

Biosynthesis Gene Cluster ◽

Streptomyces Sp ◽

Peptide Synthetase ◽

Biosynthesis Gene

ABSTRACT The main siderophores produced by streptomycetes are desferrioxamines. Here we show that Streptomyces sp. ATCC 700974 and several Streptomyces griseus strains, in addition, synthesize a hitherto unknown siderophore with a catechol-peptide structure, named griseobactin. The production is repressed by iron. We sequenced a 26-kb DNA region comprising a siderophore biosynthetic gene cluster encoding proteins similar to DhbABCEFG, which are involved in the biosynthesis of 2,3-dihydroxybenzoate (DHBA) and in the incorporation of DHBA into siderophores via a nonribosomal peptide synthetase. Adjacent to the biosynthesis genes are genes that encode proteins for the secretion, uptake, and degradation of siderophores. To correlate the gene cluster with griseobactin synthesis, the dhb genes in ATCC 700974 were disrupted. The resulting mutants no longer synthesized DHBA and griseobactin; production of both was restored by complementation with the dhb genes. Heterologous expression of the dhb genes or of the entire griseobactin biosynthesis gene cluster in the catechol-negative strain Streptomyces lividans TK23 resulted in the synthesis and secretion of DHBA or griseobactin, respectively, suggesting that these genes are sufficient for DHBA and griseobactin biosynthesis. Griseobactin was purified and characterized; its structure is consistent with a cyclic and, to a lesser extent, linear form of the trimeric ester of 2,3-dihydroxybenzoyl-arginyl-threonine complexed with aluminum under iron-limiting conditions. This is the first report identifying the gene cluster for the biosynthesis of DHBA and a catechol siderophore in Streptomyces.

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