Construction of an RAPD linkage map and localization of QTLs for oleic acid level using recombinant inbreds in mustard (Brassica juncea)

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 467-472 ◽  
Author(s):  
R Sharma ◽  
R AK Aggarwal ◽  
R Kumar ◽  
T Mohapatra ◽  
R P Sharma
Keyword(s):  
Oleic Acid ◽  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Brassica Juncea ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Acid Level ◽  
Recombinant Inbred Lines ◽  
Phenotypic Variance ◽  
Trait Loci

RAPD markers were employed for construction of a linkage map and localization of QTLs for oleic acid level using a set of 94 recombinant inbred lines (RILs) of mustard (Brassica juncea L.) as a mapping population. Only 30% of the 235 random primers used were useful in terms of polymorphism detected and the reproducibility of those patterns. Normal Mendelian segregation was observed for the majority of the 130 markers obtained with 71 informative primers; only 13.1% deviated (P < 0.01) from the expected 1:1 ratio. One-hundred and fourteen markers were assigned to 21 linkage groups (LGs) covering a total length of 790.4 cM with an average distance of 6.93 cM between markers. Two quantitative trait loci (QTL) for oleic acid level were mapped to 14- and 10.6-cM marker intervals on two different LGs. Both loci together explained 32.2% of phenotypic variance. One major QTL explained 28.5% of the trait variance observed in this species.Key words: Brassica juncea, linkage map, oleic acid, quantitative trait loci, RAPD, recombinant inbred line.

scholarly journals Detection of Quantitative Trait Loci (QTL) Associated with the Fruit Morphology of Tomato

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1117
Author(s):  
Pragya Adhikari ◽  
James McNellie ◽  
Dilip R. Panthee
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Tomato Fruit ◽  
Snp Array ◽  
Fruit Shape ◽  
Phenotypic Variance ◽  
Fruit Morphology ◽  
Trait Loci ◽  
Shape And Size

Tomato (Solanum lycopersicum L.) is the second most-consumed vegetable in the world. The market value and culinary purpose of tomato are often determined by fruit size and shape, which makes the genetic improvement of these traits a priority for tomato breeders. The main objective of the study was to detect quantitative trait loci (QTL) associated with the tomato fruit shape and size. The use of elite breeding materials in the genetic mapping studies will facilitate the detection of genetic loci of direct relevance to breeders. We performed QTL analysis in an intra-specific population of tomato developed from a cross between two elite breeding lines NC 30P × NC-22L-1(2008) consisting of 110 recombinant inbred lines (RIL). The precision software Tomato Analyzer (TA) was used to measure fruit morphology attributes associated with fruit shape and size traits. The RIL population was genotyped with the SolCAP 7720 SNP array. We identified novel QTL controlling elongated fruit shape on chromosome 10, explaining up to 24% of the phenotypic variance. This information will be useful in improving tomato fruit morphology traits.

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