Mapping of QTLs for androgenetic response based on a molecular genetic map of ×TriticosecaleWittmack

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 999-1009 ◽  
Author(s):  
Juan M González ◽  
Luis M Muñiz ◽  
Nicolás Jouve
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
Dh Population ◽  
Green Plants ◽  
Embryo Cultures ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Molecular Genetic Map ◽  
Total Influence ◽  
Dh Lines

Quantitative trait loci (QTLs) for androgenetic response were mapped in a doubled haploid (DH) population derived from the F1hybrid of 2 unrelated varieties of triticale, 'Torote' and 'Presto'. A molecular marker linkage map of this cross was previously constructed using 73 DH lines. This map contains 356 markers (18 random amplified 5polymorphic DNA, 40 random amplified microsatellite polymorphics, 276 amplified fragment length polymorphisms, and 22 simple sequence repeats) and was used for QTL analysis. The genome was well covered, and of the markers analysed, 336 were located in 21 linkage groups (81.9%) identified using SSR markers. The map covered a total length of 2465.4 cM with an average of 1 marker for each 6.9 cM. The distribution of the markers was not homogeneous across the 3 genomes, with 50.7% detected in the R genome. Several QTLs were found for the following variables related to the androgenetic response: number of embryos/100 anthers; plants regenerated from 100 embryos; number of green plants/total number of plants; and number of green plants/1000 anthers. Two were detected on chromosome 6B and 4R, which together had a 30% total influence on the induction of embryos. Another was found on 6B and on the unidentified LG1; these influenced the production of total plants from haploid embryo cultures. One QTL on chromosome 3R determined the photosynthetic viability of the haploid plantlets regenerated from microspores. Other QTLs were found on chromosomes 1B, 1R, 4R, and 7R, which helped the control of the final androgenetic response (the number of plantlets obtained for every 1000 anthers cultured).Key words: triticale, genetic map, AFLP, RAMP, RAPD, SSR, QTL, androgenesis.

A high-density molecular genetic map around the weaver locus

1996 ◽  
Vol 7 (8) ◽  
pp. 616-618 ◽  
Author(s):  
J. H. Millonig ◽  
K. J. Millen ◽  
M. E. Hatten
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
High Density ◽  
Molecular Genetic Map

scholarly journals A high-density molecular genetic map around the weaver locus

1996 ◽  
Vol 7 (12) ◽  
pp. 926-926 ◽  
Author(s):  
J. H. Millonig ◽  
K. J. Millen ◽  
M. E. Hatten
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
High Density ◽  
Molecular Genetic Map

scholarly journals An SSR-based molecular genetic map of cassava

Euphytica ◽  
2006 ◽  
Vol 147 (3) ◽  
pp. 433-440 ◽  
Author(s):  
E. Okogbenin ◽  
J. Marin ◽  
M. Fregene
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
Molecular Genetic Map

Molecular Genetic Map Construction and QTL Analysis for Fruit Maturation Period in Litchi

2011 ◽  
Vol 25 (2) ◽  
pp. 2315-2320 ◽  
Author(s):  
Y.H. Zhao ◽  
Y.S. Guo ◽  
J.X. Fu ◽  
S.S. Huang ◽  
B.B. Lu ◽  
...  
Keyword(s):  
Genetic Map ◽  
Qtl Analysis ◽  
Molecular Genetic ◽  
Fruit Maturation ◽  
Maturation Period ◽  
Map Construction ◽  
Molecular Genetic Map

Analysis of QTL for Three Leaf Traits in Phalaenopsis based on AFLP Molecular Genetic Map

2012 ◽  
Author(s):  
Shenping Xu ◽  
Xiaorong Liu ◽  
Jingmei Liu ◽  
Guoping Wang ◽  
Fangqing Lian ◽  
...  
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
Leaf Traits ◽  
Molecular Genetic Map

The identification of restriction fragment length polymorphisms linked to seed colour genes in Brassica napus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 534-542 ◽  
Author(s):  
A. E. Van Deynze ◽  
B. S. Landry ◽  
K. P. Pauls
Keyword(s):  
Brassica Napus ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rflp Markers ◽  
Seed Colour ◽  
Dh Population ◽  
Length Polymorphisms ◽  
Dh Lines ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) linked to genes controlling seed colour were identified in rapeseed (Brassica napus). The efficiency of the RFLP analysis was enhanced by utilizing bulked segregant analysis, DNA clones that had previously been used to construct a RFLP map of B. napus, and a doubled-haploid (DH) population segregating for seed colour. Markers for two of the three seed colour genes segregating in the DH population were identified on the basis of χ2 analyses of marker distributions among visually classified black-, brown-, and yellow-seeded DH lines as well as ANOVA and quantitative trait locus analysis of light-reflectance measurements from seeds of the DH lines. The RFLP markers linked to seed colour that were identified in the present study will allow breeding strategies based on genotype selection to be developed for seed colour in rapeseed.Key words: RFLP markers, seed colour genes, rapeseed.

scholarly journals Construction of a molecular genetic map for hawthorn based on SRAP markers

2015 ◽  
Vol 29 (3) ◽  
pp. 441-447 ◽  
Author(s):  
Gang Wang ◽  
Yinshan Guo ◽  
Yuhui Zhao ◽  
Kai Su ◽  
Jijun Zhang
Keyword(s):  
Genetic Map ◽  
Molecular Genetic ◽  
Srap Markers ◽  
Molecular Genetic Map

scholarly journals Expanded Genetic Map of Gibberella moniliformis (Fusarium verticillioides)

2002 ◽  
Vol 68 (4) ◽  
pp. 1972-1979 ◽  
Author(s):  
James E. Jurgenson ◽  
Kurt A. Zeller ◽  
John F. Leslie
Keyword(s):  
Genetic Map ◽  
Fragment Length ◽  
Fusarium Verticillioides ◽  
Pathogenic Fungi ◽  
Aflp Markers ◽  
Crop Species ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Further Development ◽  
Gibberella Moniliformis

ABSTRACT Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food- and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processors. The dissection of important biological characters in this fungus has been hampered by the lack of a uniformly dense genetic map. The existing restriction fragment length polymorphism-based map contains significant gaps, making it difficult to routinely locate biologically important genes, such as those involved in pathogenicity or mycotoxin production, with precision. We utilized amplified fragment length polymorphisms (AFLPs) to saturate the existing genetic map and added 486 AFLP markers to the ∼150 markers on the existing map. The resulting map has an average marker interval of 3.9 map units and averages ∼21 kb/map unit. The additional markers expanded the map from 1,452 to 2,188 map units distributed across 12 chromosomes. The maximum distance between adjacent markers is 29 map units. We identified AFLP markers less than 1 map unit from the mating type (MAT) locus and 2.5 map units from the spore killer (SK) locus; eight AFLP markers map within 8.5 units of the FUM1 (fumonisin biosynthetic) locus. The increased saturation of this map will facilitate further development of G. moniliformis as a model system for the genetic and population genetic studies of related, but less genetically tractable, plant pathogenic fungi.

scholarly journals A Molecular Genetic Map and Electrophoretic Karyotype of the Plant Pathogenic Fungus Cochliobolus sativus

2002 ◽  
Vol 15 (5) ◽  
pp. 481-492 ◽  
Author(s):  
Shaobin Zhong ◽  
Brian J. Steffenson ◽  
J. Patrick Martinez ◽  
Lynda M. Ciuffetti
Keyword(s):  
Genetic Map ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Molecular Genetic ◽  
Aflp Markers ◽  
Cochliobolus Sativus ◽  
Electrophoretic Karyotype ◽  
Linkage Groups ◽  
Molecular Genetic Map ◽  
Map Distance

A molecular genetic map was constructed and an electrophoretic karyotype was resolved for Cochliobolus sativus, the causal agent of spot blotch of barley and wheat. The genetic map consists of 27 linkage groups with 97 amplified fragment length polymorphism (AFLP) markers, 31 restriction fragment length polymorphism (RFLP) markers, two polymerase chain reaction amplified markers, the mating type locus (CsMAT), and a gene (VHv1) conditioning high virulence on barley cv. Bowman. These linkage groups covered a map distance of 849 cM. The virulence gene VHv1 cosegregated with six AFLP markers and was mapped on one of the major linkage groups. Fifteen chromosome-sized DNAs were resolved in C. sativus isolates ND93-1 and ND90Pr with contour-clamped homogeneous electric field (CHEF) electrophoresis combined with telo-mere probe analysis of comigrating chromosome-sized DNAs. The chromosome sizes ranged from 1.25 to 3.80 Mbp, and the genome size of the fungus was estimated to be approximately 33 Mbp. By hybridizing genetically mapped RFLP and AFLP markers to CHEF blots, 25 of the 27 linkage groups were assigned to specific chromosomes. The barley-specific virulence locus VHv1 was localized on a chromosome of 2.80 Mbp from isolate ND90Pr in the CHEF gel. The total map length of the fungus was estimated to be at least 1,329 cM based on the map distance covered by the linked markers and the estimated gaps. Therefore, the physical to genetic distance ratio is approximately 25 kb/cM. Construction of a high-resolution map around target loci will facilitate the cloning of the genes conferring virulence and other characters in C. sativus by a map-based cloning strategy.

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