qtl analysis
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2022 ◽  
Vol 185 ◽  
pp. 111771
Author(s):  
Hongbin Yang ◽  
Yunqian Zou ◽  
Xin Li ◽  
Mingfei Zhang ◽  
Zhifeng Zhu ◽  
...  

2022 ◽  
Vol 293 ◽  
pp. 110726
Author(s):  
Naveen Kalluri ◽  
Iban Eduardo ◽  
Pere Arús
Keyword(s):  

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Bailing Chen ◽  
Yulong Li ◽  
Meilin Tian ◽  
Hao Su ◽  
Wei Sun ◽  
...  

AbstractR. esculentum is a popular seafood in Asian countries and an economic marine fishery resource in China. However, the genetic linkage map and growth-related molecular markers are still lacking, hindering marker assisted selection (MAS) for genetic improvement of R. esculentum. Therefore, we firstly used 2b-restriction site-associated DNA (2b-RAD) method to sequence 152 R. esculentum specimens and obtained 9100 single nucleotide polymorphism (SNP) markers. A 1456.34 cM linkage map was constructed using 2508 SNP markers with an average interval of 0.58 cM. Then, six quantitative trait loci (QTLs) for umbrella diameter and body weight were detected by QTL analysis based on the new linkage map. The six QTLs are located on four linkage groups (LGs), LG4, LG13, LG14 and LG15, explaining 9.4% to 13.4% of the phenotypic variation. Finally, 27 candidate genes in QTLs regions of LG 14 and 15 were found associated with growth and one gene named RE13670 (sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1-like) may play an important role in controlling the growth of R. esculentum. This study provides valuable information for investigating the growth mechanism and MAS breeding in R. esculentum.


2021 ◽  
Vol 12 ◽  
Author(s):  
Nitika Sandhu ◽  
Gomsie Pruthi ◽  
Om Prakash Raigar ◽  
Mohini Prabha Singh ◽  
Kanika Phagna ◽  
...  

The phenomenal increase in the use of nitrogenous fertilizers coupled with poor nitrogen use efficiency is among the most important threats to the environment, economic, and social health. During the last 2 decades, a number of genomic regions associated with nitrogen use efficiency (NUE) and related traits have been reported by different research groups, but none of the stable and major effect QTL have been utilized in the marker-assisted introgression/pyramiding program. Compiling the data available in the literature could be very useful in identifying stable and major effect genomic regions associated with the root and NUE-related trait improving the rice grain yield. In the present study, we performed meta-QTL analysis on 1,330 QTL from 29 studies published in the past 2 decades. A total of 76 MQTL with a stable effect over different genetic backgrounds and environments were identified. The significant reduction in the confidence interval of the MQTL compared to the initial QTL resulted in the identification of annotated and putative candidate genes related to the traits considered in the present study. A hot spot region associated with correlated traits on chr 1, 4, and 8 and candidate genes associated with nitrate transporters, nitrogen content, and ammonium uptake on chromosomes 2, 4, 6, and 8 have been identified. The identified MQTL, putative candidate genes, and their orthologues were validated on our previous studies conducted on rice and wheat. The research-based interventions such as improving nitrogen use efficiency via identification of major genomic regions and candidate genes can be a plausible, simple, and low-cost solution to address the challenges of the crop improvement program.


2021 ◽  
Vol 12 ◽  
Author(s):  
Brigitte Uwimana ◽  
Gerald Mwanje ◽  
Michael Batte ◽  
Violet Akech ◽  
Trushar Shah ◽  
...  

The first step toward marker-assisted selection is linking the phenotypes to molecular markers through quantitative trait loci (QTL) analysis. While the process is straightforward in self-pollinating diploid (2x) species, QTL analysis in polyploids requires unconventional methods. In this study, we have identified markers associated with weevil Cosmopolites sordidus (Germar) resistance in bananas using 138 triploid (2n = 3x) hybrids derived from a cross between a tetraploid “Monyet” (2n = 4x) and a 2x “Kokopo” (2n = 2x) banana genotypes. The population was genotyped by Diversity Arrays Technology Sequencing (DArTSeq), resulting in 18,009 polymorphic single nucleotide polymorphisms (SNPs) between the two parents. Marker–trait association was carried out by continuous mapping where the adjusted trait means for the corm peripheral damage (PD) and total cross-section damage (TXD), both on the logit scale, were regressed on the marker allele frequencies. Forty-four SNPs that were associated with corm PD were identified on the chromosomes 5, 6, and 8, with 41 of them located on chromosome 6 and segregated in “Kokopo.” Eleven SNPs associated with corm total TXD were identified on chromosome 6 and segregated in “Monyet.” The additive effect of replacing one reference allele with the alternative allele was determined at each marker position. The PD QTL was confirmed using conventional QTL linkage analysis in the simplex markers segregating in “Kokopo” (AAAA × RA). We also identified 43 putative genes in the vicinity of the markers significantly associated with the two traits. The identified loci associated with resistance to weevil damage will be used in the efforts of developing molecular tools for marker-assisted breeding in bananas.


2021 ◽  
Vol 21 ◽  
pp. 100855
Author(s):  
Stavroula Oikonomou ◽  
Alexandros Tsakogiannis ◽  
Christina Kriaridou ◽  
Theodoros Danis ◽  
Tereza Manousaki ◽  
...  

2021 ◽  
Vol 41 (10) ◽  
Author(s):  
Weijun Ye ◽  
Yong Yang ◽  
Peiran Wang ◽  
Yin Zhang ◽  
Liya Zhang ◽  
...  

2021 ◽  
Author(s):  
Yuriy Chesnokov ◽  

A possible relationship between the approaches of adaptive crop production and precision agriculture to produce environmentally friendly lines and varieties with high adaptive potential and productivity is shown. In recent decades, more and more attention has been paid to technogenic and biological systems of farming, based on the ecologization and biologization of the intensification processes of adaptive crop produc-tion. Such approaches are precision agriculture (PA) system and QTL analysis. The use of these approaches allows not only to ensure a sustainable increase in productivity through the combined use of the advantages of precision agriculture and molecular genetic assessment, including the creation of new forms and varieties re-sponsive to agricultural practices of PA, but also to neutralize the negative impact of abiotic and biotic envi-ronmental factors limiting the size and quality of the crop as well as plant productivity.


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