POLYMORPHISM IN THE GIEMSA C-BANDING PATTERN OF RYE CHROMOSOMES

1978 ◽  
Vol 20 (3) ◽  
pp. 307-312 ◽  
Author(s):  
T. Lelley ◽  
K. Josifek ◽  
P. J. Kaltsikes

Extensive polymorphism was found with regard to the presence and size of Giemsa-staining bands in the chromosomes of six inbred lines of cultivated rye (Secale cereale L.). The amount of polymorphism differed from chromosome to chromosome, with 6R being the most variable and 3R or 7R the least.

1978 ◽  
Vol 20 (2) ◽  
pp. 199-204 ◽  
Author(s):  
J. P. Gustafson ◽  
K. D. Krolow

Three tetraploid triticales were analysed by C-banding techniques in order to establish their chromosome constitutions. All three tetraploid triticales contained seven rye chromosomes with the banding pattern of Secale cereale L. A mixture of A- and B-genome chromosomes from Triticum turgidum L. constituted the wheat genome present in the tetraploid triticales. Triticale Trc 4x3 contained 1A, 2B, 3A, 4A, 5B, 6A, and 7B. Triticale Trc 4x2 contained 1A, 2B, 3B, 4B, 5B, 6A, and 7B, while triticale Trc 4x5 contained 1A, 2B, 3B, 4A, 5A, 6A, and 7B. The reliability of the staining technique is subject to errors in identification, which are discussed.


1976 ◽  
Vol 18 (3) ◽  
pp. 491-496 ◽  
Author(s):  
A. E. Limin ◽  
J. Dvořák

Various times, temperatures and concentrations of SSC were tested in an attempt to elucidate the mechanism of C-banding in plants. It is shown that C-bands can be induced in rye (Secale cereale L.) chromosomes by SSC treatment at temperatures as low as 0 °C for periods as short as 1 min, an effect previously unknown in either plants or animals. Barium hydroxide treatment appears to be essential for the production of bands. If chromosomes are treated with SSC omitting the Ba(OH)2 treatment, relatively uniform loss of nucleoproteins occurs without the production of C-bands. It is suggested that Ba(OH)2 alters the chemical structure of nucleoproteins in heterochromatin rendering them insoluble in SSC. It is unlikely that SSC functions as a DNA reassociation agent in the production of C-bands. More likely it functions as a leaching agent which extracts soluble nucleoproteins from the chromosomes. Incubation in 2 × SSC at room temperatures for 5-10 min was found to be sufficient for the production of a well contrasted banding pattern in rye chromosomes.


1979 ◽  
Vol 21 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. J. Scoles ◽  
L. E. Evans

Three inbred lines of rye (Secale cereale L.) known to be capable of restoring fertility to a cytoplasmic male-sterile line were crossed with the sterile line. The proportions of male fertile, partially male fertile and male sterile plants in F2 and backcross progenies indicated that three dominant restorer genes were present in each line. These were designated Rf1, Rf2 and Rf3, their relative expressivity was Rf1>Rf2>Rf3. Expressivity was dependent upon environment. Partial fertility occurred when certain genotypes carried two of the three alleles as dominant, but was dependent upon genotype and environment.


Genetica ◽  
1994 ◽  
Vol 92 (3) ◽  
pp. 149-154 ◽  
Author(s):  
M. M. Jim�nez ◽  
E. Romera ◽  
M. J. Puertas ◽  
R. N. Jones

1983 ◽  
Vol 25 (6) ◽  
pp. 668-670 ◽  
Author(s):  
Graham J. Scoles

Five inbred lines of rye (Secale cereale L.) and an open-pollinated rye cultivar were used to pollinate wheat (Triticum aestivum L.) cultivars of differeing crossability. No hybrid seed was produced with the cultivar of low crossability, but with the highly crossable cultivar an average seed set of 65% was obtained. Significant differences in terms of seed set and weight of F1 seed were detected among the inbreds.


2014 ◽  
Vol 50 (4) ◽  
pp. 567-574
Author(s):  
Helena Kubicka ◽  
Bogusław Kubicki

Brittlenes of thie stem was found to be one of the more frequently segregating traits in inbred rye lines. In dependenece on the outset cultivated variety this trait appears in the S<sub>2</sub> generation in 4.0-0.6 per cent of the inbred lines. Genetical analysis demonstrated that the trait of brttlenes is determined by one recessive gene denoted by the symbol b<sub>s</sub> (brittlee stem). The bs gene exerts a strong pleliotropic effect on the whole plant, beside brittleness of the stem it causes fragility of the roots, heads and leaves and depresses the general viability of the plants. Anatomilcal observations of thie stem and root sholwed that the b<sub>s</sub> gene causes disturbances in the norirmal lignifcation of the sclerenichyma cells both in shoots and roots, so that these cells are th1i-walled. The tickness of the sclerenchyma layers and the number and size of the vasclcuar in the brittle forms are significantly smaller than in the normal ones. It was found that these changes appear in the bittle forms at the phase of heading of the plants.


1979 ◽  
Vol 59 (1) ◽  
pp. 163-169 ◽  
Author(s):  
G. J. SCOLES ◽  
L. E. EVANS

Six inbred lines of spring rye (Secale cereale L.) and an open-pollinated cultivar of spring rye were crossed with a cytoplasmic male-sterile line of winter rye. The open-pollinated cultivar and all except one inbred line exhibited segregation for fertility restoration. Through further inbreeding, sub-lines of certain breeds were obtained which were homozygous for maintenance of sterility or for restoration of fertility. Environmental effects on the expression of fertility restoration were detected.


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