Impact of Irrigation Erosion on the Agrochemical Properties of Mountain-brown Soils and the Productivity of Cereals and Legumes

The cultivation of perennial grasses in mountainous areas protects the slopes from erosion and provides animals with good food. According to the results of the studies, it was determined that the restoration of fertility and the ecological balance of erosional lands and the implementation of soil and agrotechnical measures is of great importance for increasing yields. Thanks to the application of these measures, as a result of the improvement of the water-physical properties of the affected lands, runoff of surface waters is prevented. Perennial grasses accumulate the nitrogen atmosphere of legumes, enrich the soil with organic substances, accelerate the formation of a water-resistant granular-fuel structure and improve its water-physical properties, which in turn contributes to the rapid assimilation of minerals.

An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background

Plant breeding relies on the meiotic recombination or crossing over to generate the new combinations of the alleles along and among the chromosomes. However, crossing over is constrained in the crops such as barley by a combination of the low frequency and biased distribution. In this study, we attempted to identify the genes that limit the recombination by performing a suppressor screen for the restoration of fertility to the semi-fertile barley mutant desynaptic10 (des10), carrying a mutation in the barley ortholog of MutL-Homolog 3 (HvMLH3), a member of the MutL-homolog (MLH) family of DNA mismatch repair genes. des10 mutants exhibit reduced recombination and fewer chiasmata, resulting in the loss of obligate crossovers (COs) leading to chromosome mis-segregation. We identified several candidate suppressor lines and confirmed their restored fertility in an Hvmlh3 background in the subsequent generations. We focus on one of the candidate suppressor lines, SuppLine2099, which showed the most complete restoration of fertility. We characterized this line by using a target-sequence enrichment and sequencing (TENSEQ) capture array representing barley orthologs of 46 meiotic genes. We found that SuppLine2099 contained a C/T change in the anti-CO gene RecQ-like helicase 4 (RECQL4) resulting in the substitution of a non-polar glycine to a polar aspartic acid (G700D) amino acid in the conserved helicase domain. Single nucleotide polymorphism (SNP) genotyping of F3 populations revealed a significant increase in the recombination frequency in lines with Hvrecql4 in the Hvmlh3 background that was associated with the restoration of fertility. The genotyping also indicated that there was nearly double the recombination levels in homozygous Hvrecql4 lines compared to the wild type (WT). However, we did not observe any significant change in the distribution of CO events. Our results confirm the anti-CO role of RECQL4 in a large genome cereal and establish the possibility of testing the utility of increasing recombination in the context of traditional crop improvement.

Complete Purging of Ewing Sarcoma Metastases from Human Ovarian Cortex Tissue Fragments by Inhibiting the mTORC1 Signaling Pathway

Restoration of fertility by autologous transplantation of ovarian cortex tissue in former cancer patients may lead to the reintroduction of malignancy via the graft. Pharmacological ex vivo purging of ovarian cortex fragments prior to autotransplantation may reduce the risk of reseeding the cancer. In this study we have investigated the capacity of Everolimus (EVE), an inhibitor of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, to eradicate Ewing’s sarcoma (ES) from ovarian tissue by a short-term ex vivo treatment. Exposure of experimentally induced ES tumor foci in ovarian tissue to EVE for 24 h completely eliminated the malignant cells without detrimental effects on follicle morphology, survival or early folliculogenesis. This indicates that effective purging of ovarian cortex tissue from contaminating ES tumor foci is possible by short-term exposure to EVE.

Altering the Binding Properties of PRDM9 Partially Restores Fertility across the Species Boundary

Sterility or subfertility of male hybrid offspring is commonly observed. This phenomenon contributes to reproductive barriers between the parental populations, an early step in the process of speciation. One frequent cause of such infertility is a failure of proper chromosome pairing during male meiosis. In subspecies of the house mouse, the likelihood of successful chromosome synapsis is improved by the binding of the histone methyltransferase PRDM9 to both chromosome homologs at matching positions. Using genetic manipulation, we altered PRDM9 binding to occur more often at matched sites, and find that chromosome pairing defects can be rescued, not only in an intersubspecific cross, but also between distinct species. Using different engineered variants, we demonstrate a quantitative link between the degree of matched homolog binding, chromosome synapsis, and rescue of fertility in hybrids between Mus musculus and Mus spretus. The resulting partial restoration of fertility reveals additional mechanisms at play that act to lock-in the reproductive isolation between these two species.

Spatio-temporal control of phenylpropanoid biosynthesis by inducible complementation of a cinnamate 4-hydroxylase mutant

Cinnamate 4-hydroxylase (C4H) is a cytochrome P450-dependent monooxygenase that catalyzes the second step of the general phenylpropanoid pathway. Arabidopsis reduced epidermal fluorescence 3 (ref3) mutants, which carry hypomorphic mutations in C4H, exhibit global alterations in phenylpropanoid biosynthesis and have developmental abnormalities including dwarfing. Here we report the characterization of a conditional Arabidopsis C4H line (ref3-2 pOpC4H), in which wild-type C4H is expressed in the ref3-2 background. Expression of C4H in plants with well-developed primary inflorescence stems resulted in restoration of fertility and the production of substantial amounts of lignin, revealing that the developmental window for lignification is remarkably plastic. Following induction of C4H expression in ref3-2 pOpC4H, we observed rapid and significant reductions in the levels of numerous metabolites, including several benzoyl and cinnamoyl esters and amino acid conjugates. These atypical conjugates were quickly replaced with their sinapoylated equivalents, suggesting that phenolic esters are subjected to substantial amounts of turnover in wild-type plants. Furthermore, using localized application of dexamethasone to ref3-2 pOpC4H, we show that phenylpropanoids are not transported appreciably from their site of synthesis. Finally, we identified a defective Casparian strip diffusion barrier in the ref3-2 mutant root endodermis, which is restored by induction of C4H expression.

Agrochemical Idications Of The Deposits Of Azerbaijan and Effect Of Erosion Process On Plant Productivity

Taking all these into account, it is important to increase the fertility of the soil from the erosion process in Ismayilli and to prevent the washing of fodder crops from perennial herbs. The cultivation of these plants in the mountainous regions protects the slopes from the terrible erosion process and provides the animals with a strong fodder. It is proved by the results of the research that restoration of fertility and ecological balance of erosion lands and the implementation of soil-agro-technical measures to increase productivity are of great importance. Due to the application of these measures, I, as a result of improving the water and physical properties of the affected land, prevent surface water flows. In addition, the results of the study have been proven by the fact that, for certain reason, erosion and erosion hazards are most likely to be taken over by the sowing of perennial herbs. Thus, perennial herbs, in particular, accumulate the nitrogen atmosphere of the legumes, enriches the soil with organic matter, accelerates the formation of water-resistant granular - topical structure and improves its water-physical properties, which in turn facilitates the rapid digestion of foodstuffs.

AGROCHEMICAL INDICATIONS OF THE DEPOSITS OF AZERBAIJAN AND EFFECT OF EROSION PROCESS ON PLANT PRODUCTIVITY

Taking all these into account, it is important to increase the fertility of the soil from the erosion process in Ismayilli and to prevent the washing of fodder crops from perennial herbs. The cultivation of these plants in the mountainous regions protects the slopes from the terrible erosion process and provides the animals with a strong fodder. It is proved by the results of the research that restoration of fertility and ecological balance of erosion lands and the implementation of soil-agro-technical measures to increase productivity are of great importance. Due to the application of these measures, I, as a result of improving the water and physical properties of the affected land, prevent surface water flows. In addition, the results of the study have been proven by the fact that, for certain reason, erosion and erosion hazards are most likely to be taken over by the sowing of perennial herbs. Thus, perennial herbs, in particular, accumulate the nitrogen atmosphere of the legumes, enriches the soil with organic matter, accelerates the formation of water-resistant granular - topical structure and improves its water-physical properties, which in turn facilitates the rapid digestion of foodstuffs.

Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)

Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F 1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2 (875 bp) and orf456 (456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF (550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.