Characterization of a dispersed repetitive DNA sequence associated with the CCDD genome of wild rice

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 681-688 ◽  
Author(s):  
M. C. Kiefer-Meyer ◽  
A. S. Reddy ◽  
M. Delseny
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Wild Rice ◽  
Repeated Sequence ◽  
Amino Acid Sequences ◽  
Open Reading Frames ◽  
Repetitive Dna Sequence ◽  
Wild Rice Species

A HindII repetitive fragment (pOD3) was isolated and cloned from the genomic DNA of an accession of Oryza latifolia, a wild rice species that possesses a tetraploid CCDD genome. Southern blot analysis using this clone as a probe demonstrated that this repetitive DNA sequence had a dispersed organization in the CCDD genome and seemed to be highly specific for this genome type. This fragment is the first CCDD-specific repeated DNA sequence to be described. The hybridization pattern is similar for most CCDD accessions tested, although a few showed no hybridization signal. The nucleotide sequence of the element cloned in pOD3 was determined and analysed. The 1783 base pair long repeated sequence shows no homology with other known nucleotide sequences. In addition, none of the amino acid sequences deduced from the potential open reading frames contained in the pOD3 repeat is homologous to any known protein. The nucleotide sequence presents several internal repeats, direct or inverted, but their significance remains unknown.Key words: rice, dispersed repetitive DNA sequences, genome-specific sequences.

scholarly journals The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA

1980 ◽  
Vol 8 (6) ◽  
pp. 1201-1216 ◽  
Author(s):  
A.S. Krayev ◽  
D.A. Kramerov ◽  
K.G. Skryabin ◽  
A.P. Ryskov ◽  
A.A. Bayev ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

Cloning and characterisation of a new rye-specific repeated sequence

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 81-87 ◽  
Author(s):  
François Guidet ◽  
Peter Rogowsky ◽  
Christopher Taylor ◽  
Weining Song ◽  
Peter Langridge
Keyword(s):  
Molecular Marker ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Genetic Material ◽  
Repeated Sequence ◽  
Repeated Dna ◽  
New Family ◽  
Repetitive Dna Sequence ◽  
Repeated Dna Families

In this paper we describe a repetitive DNA sequence unique to the rye genome. The corresponding DNA probe (pAW173) was cloned as a 450-bp fragment and does not hybridize to wheat or barley DNA and is not related to five other repeated DNA families characterised in rye. This new family (designated the R173 family) consists of moderately repeated DNA (~ 15 000 units per genome) and is present on all seven rye chromosomes. The hybridization patterns in Southern blots indicate that it is not organised as blocks of tandem arrays and in situ hybridization shows that it is dispersed throughout the rye chromosomes. The probe has been successfully used as a molecular marker in identifying rye genetic material in a wheat background.Key words: repetitive DNA sequence, rye-specific DNA, molecular marker.

scholarly journals Characterization of an unusually conserved AluI highly reiterated DNA sequence family from the honeybee, Apis mellifera.

Genetics ◽  
1993 ◽  
Vol 134 (4) ◽  
pp. 1195-1204
Author(s):  
S Tarès ◽  
J M Cornuet ◽  
P Abad
Keyword(s):  
Apis Mellifera ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Repeated Sequence ◽  
Consensus Sequences ◽  
Dna Sequence Data ◽  
Repeat Class ◽  
Honeybee Subspecies

Abstract An AluI family of highly reiterated nontranscribed sequences has been found in the genome of the honeybee Apis mellifera. This repeated sequence is shown to be present at approximately 23,000 copies per haploid genome constituting about 2% of the total genomic DNA. The nucleotide sequence of 10 monomers was determined. The consensus sequences is 176 nucleotides long and has an A + T content of 58%. There are clusters of both direct and inverted repeats. Internal subrepeating units ranging from 11 to 17 nucleotides are observed, suggesting that it could have evolved from a shorter sequence. DNA sequence data reveal that this repeat class is unusually homogeneous compared to the other class of invertebrate highly reiterated DNA sequences. The average pairwise sequence divergence between the repeats is 2.5%. In spite of this unusual homogeneity, divergence has been found in the repeated sequence hybridization ladder between four different honeybee subspecies. Therefore, the AluI highly reiterated sequences provide a new probe for fingerprinting in A. m. mellifera.

scholarly journals Nucleotide Sequence and Genetic Structure of a Novel Carbaryl Hydrolase Gene (cehA) from Rhizobium sp. Strain AC100

2002 ◽  
Vol 68 (3) ◽  
pp. 1220-1227 ◽  
Author(s):  
Masayuki Hashimoto ◽  
Mitsuru Fukui ◽  
Kouichi Hayano ◽  
Masahito Hayatsu
Keyword(s):  
Nucleotide Sequence ◽  
Insertion Sequence ◽  
Amino Acid Sequences ◽  
Open Reading Frames ◽  
Homology Search ◽  
Sequencing Analysis ◽  
Terminal Sequence ◽  
Homologous Sequences ◽  
Naphthyl Acetate ◽  
Reading Frames

ABSTRACT Rhizobium sp. strain AC100, which is capable of degrading carbaryl (1-naphthyl-N-methylcarbamate), was isolated from soil treated with carbaryl. This bacterium hydrolyzed carbaryl to 1-naphthol and methylamine. Carbaryl hydrolase from the strain was purified to homogeneity, and its N-terminal sequence, molecular mass (82 kDa), and enzymatic properties were determined. The purified enzyme hydrolyzed 1-naphthyl acetate and 4-nitrophenyl acetate indicating that the enzyme is an esterase. We then cloned the carbaryl hydrolase gene (cehA) from the plasmid DNA of the strain and determined the nucleotide sequence of the 10-kb region containing cehA. No homologous sequences were found by a database homology search using the nucleotide and deduced amino acid sequences of the cehA gene. Six open reading frames including the cehA gene were found in the 10-kb region, and sequencing analysis shows that the cehA gene is flanked by two copies of insertion sequence-like sequence, suggesting that it makes part of a composite transposon.

scholarly journals Characterisation of a novel repetitive DNA sequence fromMycobacterium bovis

1992 ◽  
Vol 96 (2-3) ◽  
pp. 179-185 ◽  
Author(s):  
T.J. Doran ◽  
A.L.M. Hodgson ◽  
J.K. Davies ◽  
A.J. Radford
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

scholarly journals C-Ag+−C based repetitive DNA sequence

2020 ◽  
Vol 1679 ◽  
pp. 022049
Author(s):  
M A Popova ◽  
V I Rolich ◽  
R R Ramazanov ◽  
N A Kasyanenko ◽  
P A Sokolov
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

scholarly journals Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome

1988 ◽  
Vol 16 (17) ◽  
pp. 8351-8360 ◽  
Author(s):  
Beatrice Neuer-Nitsche ◽  
Xiang Lu ◽  
Dieter Werner
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Functional Role ◽  
Mouse Genome ◽  
Repetitive Dna Sequence ◽  
Highly Repetitive Dna

Cloning and characterization of repetitive DNA sequences from genomes of Oryza minuta and Oryza australiensis

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 790-798 ◽  
Author(s):  
H. Aswidinnoor ◽  
R. J. Nelson ◽  
J. F. Dallas ◽  
C. L. McIntyre ◽  
H. Leung ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Repetitive Sequences ◽  
Rice Genome ◽  
Repetitive Dna Sequences ◽  
Cross Hybridization ◽  
Oryza Minuta ◽  
Wild Rice Species ◽  
Oryza Australiensis

The value of genome-specific repetitive DNA sequences for use as molecular markers in studying genome differentiation was investigated. Five repetitive DNA sequences from wild species of rice were cloned. Four of the clones, pOm1, pOm4, pOmA536, and pOmPB10, were isolated from Oryza minuta accession 101141 (BBCC genomes), and one clone, pOa237, was isolated from Oryza australiensis accession 100882 (EE genome). Southern blot hybridization to different rice genomes showed strong hybridization of all five clones to O. minuta genomic DNA and no cross hybridization to genomic DNA from Oryza sativa (AA genome). The pOm1 and pOmA536 sequences showed cross hybridization only to all of the wild rice species containing the C genome. However, the pOm4, pOmPB10, and pOa237 sequences showed cross hybridization to O. australiensis genomic DNA in addition to showing hybridization to the O. minuta genomic DNA.Key words: rice, genome-specific repetitive sequences, Oryza.

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