Prophage-mediated production of a bacteriocinlike substance by SPβ lysogens of Bacillus subtilis

1980 ◽  
Vol 26 (11) ◽  
pp. 1328-1333 ◽  
Author(s):  
H. Ernest Hemphill ◽  
Irene Gage ◽  
Stanley A. Zahler ◽  
Ruth Z. Korman
Keyword(s):  
Bacillus Subtilis ◽  
Temperate Bacteriophage ◽  
Bacterial Gene ◽  
Phage Gene ◽  
Subtilis Chromosome ◽  
Phage Production

Cultures of Bacillus subtilis lysogenic for the temperate bacteriophage SPβ release "betacin," a bacteriocinlike substance that inhibits B. subtilis strains which do not carry this phage. Production of betacin is blocked by mutations in the bet gene on the prophage and a second phage gene, tol, is apparently involved in making the lysogen itself tolerant to betacin. Mutations in a bacterial gene betR, located on the B. subtilis chromosome between metC and pyrD, render nonlysogens tolerant to betacin.

scholarly journals Designed Gene Amplification on the Bacillus subtilis Chromosome

Microbiology ◽  
1988 ◽  
Vol 134 (1) ◽  
pp. 85-95 ◽  
Author(s):  
M. MORI ◽  
K.-I. HASHIGUCHI ◽  
K. YODA ◽  
M. YAMASAKI
Keyword(s):  
Bacillus Subtilis ◽  
Gene Amplification ◽  
Subtilis Chromosome ◽  
Bacillus Subtilis Chromosome

On the bidirectional replication of the Bacillus subtilis chromosome

1975 ◽  
Vol 99 (2) ◽  
pp. 347-348 ◽  
Author(s):  
M. Aideen O'Sullivan ◽  
Karen Howard ◽  
Noboru Sueoka
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Chromosome ◽  
Bacillus Subtilis Chromosome

scholarly journals A 22 kb DNA sequence in the cspB-glpPFKD region at 75  on the Bacillus subtilis chromosome

Microbiology ◽  
1996 ◽  
Vol 142 (11) ◽  
pp. 3021-3026 ◽  
Author(s):  
M. A. Noback ◽  
P. Terpstra ◽  
S. Holsappel ◽  
G. Venema ◽  
S. Bron
Keyword(s):  
Bacillus Subtilis ◽  
Dna Sequence ◽  
Subtilis Chromosome ◽  
Bacillus Subtilis Chromosome

scholarly journals Xenogeneic modulation of the ClpCP protease of Bacillus subtilis by a phage-encoded adaptor-like protein

2019 ◽  
Vol 294 (46) ◽  
pp. 17501-17511 ◽  
Author(s):  
Nancy Mulvenna ◽  
Ingo Hantke ◽  
Lynn Burchell ◽  
Sophie Nicod ◽  
David Bell ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Gene Products ◽  
Bacterial Host ◽  
Systematic Analysis ◽  
Phage Gene ◽  
Cellular Processes ◽  
Bacteriophage Infection ◽  
Archaeal Viruses ◽  
Cytotoxic Proteins ◽  
Cellular Pathways

Like eukaryotic and archaeal viruses, which coopt the host's cellular pathways for their replication, bacteriophages have evolved strategies to alter the metabolism of their bacterial host. SPO1 bacteriophage infection of Bacillus subtilis results in comprehensive remodeling of cellular processes, leading to conversion of the bacterial cell into a factory for phage progeny production. A cluster of 26 genes in the SPO1 genome, called the host takeover module, encodes for potentially cytotoxic proteins that specifically shut down various processes in the bacterial host, including transcription, DNA synthesis, and cell division. However, the properties and bacterial targets of many genes of the SPO1 host takeover module remain elusive. Through a systematic analysis of gene products encoded by the SPO1 host takeover module, here we identified eight gene products that attenuated B. subtilis growth. Of the eight phage gene products that attenuated bacterial growth, a 25-kDa protein called Gp53 was shown to interact with the AAA+ chaperone protein ClpC of the ClpCP protease of B. subtilis. Our results further reveal that Gp53 is a phage-encoded adaptor-like protein that modulates the activity of the ClpCP protease to enable efficient SPO1 phage progeny development. In summary, our findings indicate that the bacterial ClpCP protease is the target of xenogeneic (dys)regulation by a SPO1 phage–derived factor and add Gp53 to the list of antibacterial products that target bacterial protein degradation and therefore may have utility for the development of novel antibacterial agents.

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