Inheritance of mitochondrial DNA in interspecific crosses of Piceaglauca and Piceaomorika

1996 ◽  
Vol 26 (3) ◽  
pp. 428-432 ◽  
Author(s):  
Andrew J. David ◽  
Daniel E. Keathley
Keyword(s):  
Mitochondrial Dna ◽  
Interspecific Hybrids ◽  
Maternal Inheritance ◽  
White Spruce ◽  
Mitochondrial Genes ◽  
Interspecific Crosses ◽  
Length Polymorphisms ◽  
Dna Restriction ◽  
Total Dna ◽  
Serbian Spruce

Fifteen interspecific hybrids of Serbian spruce (Piceaomorika (Panc) Purkyne) and white spruce (Piceaglauca (Moench) Voss) representing five separate crosses, including reciprocals, were used to demonstrate maternal inheritance of mitochondrial DNA. Total DNA was extracted from foliage samples of Serbian spruce (S), white spruce (W), and both S(♂) × W(♀) and W(♂) × S(♀) hybrids, digested and probed with one of two maize mitochondrial genes, ATPaseα or COXII. ATPaseα generated diagnostic Serbian and white spruce genotypes for all five enzymes tested, while COXII differentiated between the two species for four of five enzymes. Maternal inheritance was indicated in all hybrids for every diagnostic enzyme–probe combination. No paternal or nonparental bands were detected. A dilution experiment indicated that the Serbian and white spruce mitochondrial DNA restriction fragment length polymorphisms could be detected in as little as 60 and 500 ng of total DNA, respectively. It appears that the mechanism that controls the inheritance of mitochondria in Picea is still functional in wide interspecific crosses.

Mitochondrial DNA Restriction Fragment Length Polymorphisms in Malus Species

1993 ◽  
Vol 111 (2) ◽  
pp. 162-165 ◽  
Author(s):  
S. Kato ◽  
S. Ishikawa ◽  
S. Imakawa ◽  
S. Komori ◽  
T. Mikami ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Malus Species ◽  
Length Polymorphisms ◽  
Dna Restriction ◽  
Restriction Fragment Length

scholarly journals Molecular phylogeny of the Taeniapterini (Diptera: Micropezidae) using nuclear and mitochondrial DNA, with a reclassification of the genus Taeniaptera Macquart

2015 ◽  
Vol 46 (5) ◽  
pp. 411-430 ◽  
Author(s):  
Morgan D. Jackson ◽  
Stephen A. Marshall ◽  
Jeffrey H. Skevington
Keyword(s):  
Mitochondrial Dna ◽  
Bayesian Inference ◽  
Molecular Phylogeny ◽  
Maximum Parsimony ◽  
New World ◽  
Molecular Data ◽  
Species Group ◽  
Mitochondrial Genes ◽  
Paraphyletic Group ◽  
Total Dna

DNA molecular data are used to generate a phylogeny for the micropezid subfamily Taeniapterinae. Thirty-two taeniapterine species were sampled, including 10 of the 20 New World genera recognized by Steyskal, as well as one genus formerly treated as a synonym of Poecilotylus Hennig (Hemichaeta Steyskal). Five species from the Micropezinae were included as outgroups. A total DNA dataset of 4705 bp, including mitochondrial genes (12S and cytochrome c oxidase I (COI)) and nuclear coding genes (wingless and CAD), was analysed using maximum parsimony and Bayesian inference. The genus Taeniaptera Macquart was found to be non-monophyletic with respect to the remainder of the Taeniapterini analysed here. Taeniaptera is restricted to the Taeniaptera trivittata Macquart species group, Mitromyia Cresson is resurrected to contain the Taeniaptera grata (Wulp) species group, and Paragrallomyia Hendel is resurrected to contain most species previously considered Taeniaptera. Poecilotylus is recognized as a paraphyletic group awaiting further research.

Analysis of mitochondrial DNA restriction fragment length polymorphisms for examining genetic variability among isolates of Phellinus linteus

Mycoscience ◽  
2002 ◽  
Vol 43 (6) ◽  
pp. 443-445 ◽  
Author(s):  
Tomoyuki Nakamura ◽  
Masaki Fukuda ◽  
Tomoyuki Nakamura ◽  
Seiichi Matsugo ◽  
Yasuyuki Uzuka
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variability ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Phellinus Linteus ◽  
Length Polymorphisms ◽  
Dna Restriction ◽  
Restriction Fragment Length

scholarly journals Maternal Inheritance of Mouse mtDNA in Interspecific Hybrids: Segregation of the Leaked Paternal mtDNA Followed by the Prevention of Subsequent Paternal Leakage

Genetics ◽  
1998 ◽  
Vol 148 (2) ◽  
pp. 851-857
Author(s):  
Hiroshi Shitara ◽  
Jun-Ichi Hayashi ◽  
Sumiyo Takahama ◽  
Hideki Kaneda ◽  
Hiromichi Yonekawa
Keyword(s):  
First Generation ◽  
Maternal Inheritance ◽  
Germ Line ◽  
Interspecific Cross ◽  
F1 Hybrids ◽  
Interspecific Crosses ◽  
Female Germ Line ◽  
Total Dna ◽  
Species Specific ◽  
Pcr Techniques

Abstract The transmission profiles of sperm mtDNA introduced into fertilized eggs were examined in detail in F1 hybrids of mouse interspecific crosses by addressing three aspects. The first is whether the leaked paternal mtDNA in fertilized eggs produced by interspecific crosses was distributed stably to all tissues after the eggs' development to adults. The second is whether the leaked paternal mtDNA was transmitted to the subsequent generations. The third is whether paternal mtDNA continuously leaks in subsequent backcrosses. For identification of the leaked paternal mtDNA, we prepared total DNA samples directly from tissues or embryos and used PCR techniques that can detect a few molecules of paternal mtDNA even in the presence of 108-fold excess of maternal mtDNA. The results showed that the leaked paternal mtDNA was not distributed to all tissues in the F1 hybrids or transmitted to the following generations through the female germ line. Moreover, the paternal mtDNA leakage was limited to the first generation of an interspecific cross and did not occur in progeny from subsequent backcrosses. These observations suggest that species-specific exclusion of sperm mtDNA in mammalian fertilized eggs is extremely stringent, ensuring strictly maternal inheritance of mtDNA.

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