Enhanced Expression of Inducible Nitric Oxide Synthase by Juzen-Taiho-To in LPS-Activated RAW264.7 Cells, a Murine Macrophage Cell Line

2000 ◽  
Vol 28 (02) ◽  
pp. 217-226 ◽  
Author(s):  
Hiroshi Kawamata ◽  
Hiroshi Ochiai ◽  
Naoki Mantani ◽  
Katsutoshi Terasawa
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Raw264.7 Cells ◽  
Inos Mrna ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

We have investigated the effect of Juzen-taiho-to (TJ-48) on inducible NO synthase (iNOS) expression and nitric oxide (NO) production in RAW264.7 cells, a murine macrophage cell line. TJ-48-lipopolysaccharide (LPS) combination induced iNOS mRNA expression earlier, stronger and remained longer that paralleled but with a higher NO production compared to LPS stimulation. TJ-48 itself showed no inducible effect either no NO production or iNOS mRNA expression. This phenomenon could be considered to contribute, at least in part, to the beneficial effects of TJ-48 through the iNOS-mediated activation of biodefense mechanism.

Inducible Activity of Ginger Rhizome (Zingiber Offifinale Rosc.) on the mRNA Expression of Macrophage-Inducible Nitric Oxide (NO) Synthase and NO Production in a Macrophage Cell Line, RAW264.7 Cells

2004 ◽  
Vol 32 (05) ◽  
pp. 727-735 ◽  
Author(s):  
Nobuko Imanishi ◽  
Naoki Mantani ◽  
Shinya Sakai ◽  
Miyuki Sato ◽  
Yuko Katada ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Kinetic Studies ◽  
No Synthase ◽  
Raw264.7 Cells ◽  
No Production ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Inducible Nitric Oxide

We have investigated the effect of Zingiber offifinale Rosc. (ZOR) on macrophage-inducible nitric oxide (NO) synthase (macNOS) mRNA expression and NO production in RAW264.7 cells, a murine macrophage cell line; 100 μg/ml ZOR can induce macNOS mRNA expression, but induction effects at a dose below 10 μg/ml were weak or negligible. Kinetic studies showed that macNOS mRNA can be detected from 4 hours to 24 hours after dosing, with a peak at 8 hours. In accordance with the induction of macNOS mRNA expression, NO concentrations increased from 3.4 μM at 2 hours to almost 150 μM at 24 hours, reflecting a longer period of macNOS mRNA expression. The activity of ZOR can be considered to contribute, at least in part, to the beneficial effects of ZOR through the macNOS-mediated activation of the biodefense mechanism.

SYNERGISTIC UP-REGULATION OF IκBα mRNA EXPRESSION IN A MURINE MACROPHAGE CELL LINE FOLLOWING EXPOSURE TO GLUCAN PHOSPHATE AND ENDOTOXIN

Shock ◽  
1997 ◽  
Vol 7 (Supplement) ◽  
pp. 92
Author(s):  
David Williams ◽  
Tuanzhu Ha ◽  
Chuanfu Li ◽  
Antje Müller ◽  
John Raptis ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Line Following

Inhibition of inducible nitric oxide synthesis by oxidized lipoprotein(a) in a murine macrophage cell line

FEBS Letters ◽  
2000 ◽  
Vol 478 (1-2) ◽  
pp. 95-99 ◽  
Author(s):  
Thomas Moeslinger ◽  
Roswitha Friedl ◽  
Ivo Volf ◽  
Monika Brunner ◽  
Elisabeth Koller ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Nitric Oxide Synthesis ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Lipoprotein A ◽  
Oxidized Lipoprotein ◽  
Inducible Nitric Oxide

Effect of L-carnitine on the synthesis of nitric oxide in RAW 264·7 murine macrophage cell line

2011 ◽  
Vol 29 (8) ◽  
pp. 679-685 ◽  
Author(s):  
A. Koc ◽  
T. Ozkan ◽  
A. Z. Karabay ◽  
A. Sunguroglu ◽  
F. Aktan
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

Role of nitric oxide in hydroxyapatite-induced phagocytosis by murine macrophage cell line (RAW264.7)

2006 ◽  
Vol 51 (4) ◽  
pp. 339-344 ◽  
Author(s):  
V.K. Gopinath ◽  
M. Musa ◽  
A.R. Samsudin ◽  
P. Lalitha ◽  
W. Sosroseno
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line
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