Nitric oxide production by a murine macrophage cell line (RAW264.7 cells) stimulated with Aggregatibacter actinomycetemcomitans surface-associated material

Anaerobe ◽  
2011 ◽  
Vol 17 (5) ◽  
pp. 246-251 ◽  
Author(s):  
W. Sosroseno ◽  
P.S. Bird ◽  
G.J. Seymour
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Raw264.7 Cells ◽  
Nitric Oxide Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Oxide Production

Enhanced Expression of Inducible Nitric Oxide Synthase by Juzen-Taiho-To in LPS-Activated RAW264.7 Cells, a Murine Macrophage Cell Line

2000 ◽  
Vol 28 (02) ◽  
pp. 217-226 ◽  
Author(s):  
Hiroshi Kawamata ◽  
Hiroshi Ochiai ◽  
Naoki Mantani ◽  
Katsutoshi Terasawa
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Raw264.7 Cells ◽  
Inos Mrna ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

We have investigated the effect of Juzen-taiho-to (TJ-48) on inducible NO synthase (iNOS) expression and nitric oxide (NO) production in RAW264.7 cells, a murine macrophage cell line. TJ-48-lipopolysaccharide (LPS) combination induced iNOS mRNA expression earlier, stronger and remained longer that paralleled but with a higher NO production compared to LPS stimulation. TJ-48 itself showed no inducible effect either no NO production or iNOS mRNA expression. This phenomenon could be considered to contribute, at least in part, to the beneficial effects of TJ-48 through the iNOS-mediated activation of biodefense mechanism.

Modulation of the No Secretion in the Pichilan-Activated Murine Macrophage Cell Line, Malu

1995 ◽  
Vol 8 (3) ◽  
pp. 135-149
Author(s):  
N. Vallot ◽  
F. Boudard ◽  
M. Bastide
Keyword(s):  
Cell Line ◽  
Mechanism Of Action ◽  
Metabolic Pathways ◽  
The Other ◽  
Nitric Oxide Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Kinase C

The mechanism of action of pichilan, a (1->3)-β-D-glucan on nitric oxide production by a murine macrophage cell line, MALU cells, was examined. Different metabolic pathways were investigated in order to understand pichilan-induced NO secretion. We demonstrate in the present paper that neither the acid arachidonic metabolism, the cAMP nor Ca2+ accumulation occured in the pichilan mechanism of action on NO secretion. On the other hand, we observed that a phorbol ester, PMA, modulated the NO secretion. An inhibition of 36% of the NO secretion was observed; consequently, pichilan could regulate the NO secretion by way of the protein kinase C. Furthermore, we demonstrated that TNF production stimulated by pichilan activation induced NO secretion by MALU cells. TNF would be the main modulator of NO secretion by pichilan or LPS-activated MALU cells. Moreover, we can note that pichilan and LPS did not act similarly on nitrite secretion by MALU cells.

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