Extracellular Electron Transfer Mechanisms in a Moderately Halophilic Bacterium from the Great Salt Lake for High Salinity Heavy Metal Biosensing

An aerobic, spore-forming, moderately halophilic bacterium, strain BH260T, was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30–32 °C and pH 8·0 in the presence of 12–15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity for strain BH260T with respect to Lentibacillus salsus KCTC 3911T, Lentibacillus juripiscarius JCM 12147T and Lentibacillus salicampi KCCM 41560T were 96·0, 95·5 and 95·3 %, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1γ-meso-diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260T (=KCTC 3915T=DSM 16462T).

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Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.003624-0 ◽

2009 ◽

Vol 59 (8) ◽

pp. 2058-2063 ◽

Cited By ~ 22

Author(s):

Y.-G. Chen ◽

X.-L. Cui ◽

Y.-X. Wang ◽

Y.-Q. Zhang ◽

S.-K. Tang ◽

...

Keyword(s):

Salt Lake ◽

Halophilic Bacterium ◽

Moderately Halophilic ◽

Moderately Halophilic Bacterium

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Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63180-0 ◽

2005 ◽

Vol 55 (1) ◽

pp. 95-99 ◽

Cited By ~ 44

Author(s):

Pei-Gen Ren ◽

Pei-Jin Zhou

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Salt Lake ◽

Sequence Similarity ◽

Halophilic Bacteria ◽

Halophilic Bacterium ◽

Rrna Gene ◽

Neutral Salt ◽

Moderately Halophilic ◽

Moderately Halophilic Bacterium

Two Gram-positive, rod-shaped, spore-forming and moderately halophilic bacteria (strains 28-1T, 28-4), isolated from a soil sample from a neutral salt lake in Xin-Jiang, China, were characterized polyphasically. On the basis of fasta (ungapped) analyses of 16S rRNA gene sequences, strains 28-1T and 28-4 were shown to belong to the Bacillaceae and to be closely related to Filobacillus milensis DSM 13259T (97·0 %) and Bacillus haloalkaliphilus DSM 5271T (95·7 %). 16S rRNA gene sequence similarity with other recognized species was not more than 94·1 %. Phylogenetic, chemotaxonomic, physiological and biochemical data supported the differentiation of these novel strains from F. milensis and B. haloalkaliphilus. Therefore these two previously unidentified strains are considered to represent a new genus and species, for which the name Tenuibacillus multivorans gen. nov., sp. nov. is proposed. The type strain is 28-1T (=AS 1.3442T=NBRC 100370T).

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