Arteriovenous oximeter for O2 content difference, O2 saturations, and hemoglobin content

We combined two spectrophotometric oximeters to measure continuously and simultaneously arteriovenous O2 content difference (AVOD) as well as arterial and venous oxyhemoglobin saturations (SaO2, SvO2) and total hemoglobin concentration (Hb). AVOD of the flowing arterial and venous whole blood was determined by the method of Guyton et al. (J. Appl. Physiol. 10: 158-163, 1957) and Shepherd and Burgar [Am. J. Physiol. 232 (Heart Circ. Physiol. 1): H437-H440, 1977]. The new arteriovenous oximeter was tested in dog experiments in which SaO2, SvO2, Hb, and AVOD were variously changed by temporary suffocation, electric muscle stimulation, hemorrhage and transfusion, and hemodilution with saline. AVOD, SaO2, SvO2, and Hb were compared with the data of the arterial and venous blood sampled near the oximeter cuvettes and measured with an IL282 CO oximeter. In one dog experiment and one in vitro blood experiment, AVOD data of the same arterial and venous blood were compared by connecting the present oximeter in series with an A-VOX Systems oximeter developed by Shepherd and Burgar. The results showed that the new arteriovenous oximeter can continuously measure AVOD, SaO2, SvO2, and Hb over wide ranges with reasonable accuracy.

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Optoacoustic monitoring of total hemoglobin concentration: In vitro and in vivo studies

Biomedical Topical Meeting ◽

10.1364/bio.2004.fh4 ◽

2004 ◽

Author(s):

I. Y. Petrova ◽

D. S. Prough ◽

Y. Y. Petrov ◽

H.- P. F. Brecht ◽

C. Svensen ◽

...

Keyword(s):

Hemoglobin Concentration ◽

In Vivo Studies ◽

Total Hemoglobin ◽

Total Hemoglobin Concentration

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Oxygen affinity of blood in altitude Sherpas

Journal of Applied Physiology ◽

10.1152/jappl.1979.47.2.337 ◽

1979 ◽

Vol 47 (2) ◽

pp. 337-341 ◽

Cited By ~ 44

Author(s):

M. Samaja ◽

A. Veicsteinas ◽

P. Cerretelli

Keyword(s):

Sea Level ◽

Concentration Ratio ◽

Oxygen Affinity ◽

Hemoglobin Concentration ◽

Cell Counts ◽

Blood Cell Counts ◽

Total Hemoglobin ◽

The Mean ◽

Total Hemoglobin Concentration ◽

2,3 Dpg

Oxygen equilibrium curves on blood within 6 h from sampling have been estimated from polarographic measurements of oxyhemoglobin concentration, in 13 male 14- to 50-yr old Sherpas residing at 3,850 m above sea level (Kumjung, Nepal). In samples with red blood cell counts = 4.7 +/- 0.8 (SD) x 10(6)/mm3, total hemoglobin concentration [Hb] = 17.0 +/- 1.9 g/dl, and hematocrit = 53.3 +/- 5.0, the mean oxygen half-saturation of hemoglobin (P50) (pH = 7.4 and PCO2 = 40 Torr) was 27.3 +/- 1.8 Torr. The P50 of altitude Sherpas was not significantly different from that of acclimatized lowlanders (28.2 +/- 1.3; n = 7), sea-level Caucasian residents (26.5 +/- 1.0; n = 17), and Sherpas at sea level (27.1; n = 3). The 2,3-diphosphoglyceric acid-to-hemoglobin concentration ratio ([2,3-DPG]/[Hb]) in altitude Sherpas was 1.22 +/- 0.03, the same as that of acclimatized Caucasians (1.22 +/- 0.10). The Bohr effect measured for the blood of one altitude Sherpas by the ratio deltalog P50/deltapH was -0.32 and -0.45 at PCO2 levels of 40 and 20 Torr, respectively. These values are not significantly different from those found in Caucasians at sea level where deltalog P50/deltalpH was -0.35 and -0.42, respectively. It is concluded that the P50 in native highlanders is not significantly different from that observed in sea-level dwellers. [2,3-DPG]/[Hb] at altitude, both in natives and in newcomers, is 20% higher than in sea-level residents.

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Results of routine determination of clinically significant hemoglobin derivatives by multicomponent analysis.

Clinical Chemistry ◽

10.1093/clinchem/32.6.972 ◽

1986 ◽

Vol 32 (6) ◽

pp. 972-978 ◽

Cited By ~ 24

Author(s):

A Zwart ◽

E J van Kampen ◽

W G Zijlstra

Keyword(s):

Pulmonary Disease ◽

Fetal Hemoglobin ◽

Hemoglobin Concentration ◽

Major Surgery ◽

Chemical Laboratory ◽

Total Hemoglobin ◽

Clinically Significant ◽

Blood Specimens ◽

Total Hemoglobin Concentration

Abstract Our recently developed multiwavelength method for multi-component analysis of hemoglobin (Hb) derivatives (Clin Chem 1984;30:373-379) was adapted for routine use in the clinical chemical laboratory. The method was applied in 4066 determinations on blood specimens from patients awaiting major surgery (n = 3863) or visiting the outpatient department for pulmonary disease (n = 203). Mean total hemoglobin concentration was 141 (SD 14) g/L. The proportion of HbCO was slightly to moderately increased (1.5-10.0%) in 36.5% of all patients; in a few cases it was as high as 15%. Mean methemoglobin was 0.4% (SD 0.2%) in the surgical patients, but 1.5% (SD 0.8%) in the patients with pulmonary disease. In some patients of the latter group the proportion of methemoglobin amounted to 5%. Sulfhemoglobin was found less than 0.4% in all specimens. Interference by paraproteins and by increased concentrations of bilirubin and lipids in plasma was easily detected by means of the performance checks provided by the spectrophotometer (an HP 8451 UV/Vis). The method is equally suitable for measuring blood samples containing fetal hemoglobin.

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