scholarly journals Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Takumi Takeuchi ◽  
Masahito Takahashi ◽  
Kazuhiko Satomi ◽  
Hideaki Ohne ◽  
Atsushi Hasegawa ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Electrical Activity ◽  
Motor Function ◽  
Cervical Spinal Cord ◽  
Cervical Cord ◽  
Adult Rats ◽  
Corticospinal Tracts ◽  
Compound Muscle Action Potentials ◽  
Cord Injury ◽  
Recovery Of Motor Function

The objective of this study was to electrophysiologically assess the corticospinal tracts of adult rats and the recovery of motor function of their forelimbs after cervical cord hemisection. Of 39 adult rats used, compound muscle action potentials (CMAPs) of the forelimbs of 15 rats were evaluated, before they received left C5 segmental hemisection of the spinal cord, by stimulating the pyramid of the medulla oblongata on one side using an exciting microelectrode. All 15 rats exhibited contralateral electrical activity, but their CMAPs disappeared after hemisection. The remaining 24 rats received hemisection first, and CMAPs of 12 rats were assessed over time to study their recovery time. All of them exhibited electrical activity of the forelimbs in 4 weeks after surgery. The remaining 12 rats received additional right C2 segmental hemisection, and variation of CMAPs between before and after surgery was examined. The right side of the 12 rats that received the additional hemisection exhibited no electrical activity in response to the stimulation of the pyramids on both sides. These results suggest that changes in path between the resected and healthy sides, activation of the ventral corticospinal tracts, and propriospinal neurons were involved in the recovery of motor function after cervical cord injury.

scholarly journals Mechanism of Forelimb Motor Function Restoration after Cervical Spinal Cord Hemisection in Rats: A Comparison of Juveniles and Adults

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Atsushi Hasegawa ◽  
Masahito Takahashi ◽  
Kazuhiko Satomi ◽  
Hideaki Ohne ◽  
Takumi Takeuchi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Motor Function ◽  
Rating Scale ◽  
Cervical Spinal Cord ◽  
Age Groups ◽  
Adult Rats ◽  
Juvenile Rats ◽  
Motor Paralysis ◽  
Significant Difference ◽  
Cord Injury

The aim of this study was to investigate forelimb motor function after cervical spinal cord injury in juvenile and adult rats. Both rats received a left segmental hemisection of the spinal cord after C3-C4 laminectomy. Behavioral evaluation of motor function was monitored and assessed using the New Rating Scale (NRS) and Forelimb Locomotor Scale (FLS) and by measuring the range of motion (ROM) of both the elbow and wrist. Complete left forelimb motor paralysis was observed in both rats. The NRS showed motor function recovery restored to50.2±24.7%in juvenile rats and34.0±19.8%in adult rats. FLS was60.4±26.8%in juvenile rats and46.5±26.9%in adult rats. ROM of the elbow and wrist were88.9±20.6%and44.4±24.1%in juvenile rats and70.0±29.2%and40.0±21.1%in adult rats. Thus, the NRS and ROM of the elbow showed a significant difference between age groups. These results indicate that left hemisection of the cervical spinal cord was not related to right-sided motor functions. Moreover, while motor paralysis of the left forelimb gradually recovered in both groups, the improvement was greater in juvenile rats.

Serotonin2 receptors mediate respiratory recovery after cervical spinal cord hemisection in adult rats

2001 ◽  
Vol 91 (6) ◽  
pp. 2665-2673 ◽  
Author(s):  
Shi-Yi Zhou ◽  
Gregory J. Basura ◽  
Harry G. Goshgarian
Keyword(s):  
Spinal Cord ◽  
Receptor Antagonist ◽  
Phrenic Nerve ◽  
Cervical Spinal Cord ◽  
Sprague Dawley ◽  
Related Activity ◽  
Adult Rats ◽  
Cord Injury ◽  
Respiratory Recovery ◽  
Spinal Cord Hemisection

The aim of the present study was to specifically investigate the involvement of serotonin [5-hydroxytryptamine (5-HT2)] receptors in 5-HT-mediated respiratory recovery after cervical hemisection. Experiments were conducted on C2 spinal cord-hemisected, anesthetized (chloral hydrate, 400 mg/kg ip), vagotomized, pancuronium- paralyzed, and artificially ventilated female Sprague-Dawley rats in which CO2 levels were monitored and maintained. Twenty-four hours after spinal hemisection, the ipsilateral phrenic nerve displayed no respiratory-related activity indicative of a functionally complete hemisection. Intravenous administration of the 5-HT2A/2C-receptor agonist (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) induced respiratory-related activity in the phrenic nerve ipsilateral to hemisection under conditions in which CO2 was maintained at constant levels and augmented the activity induced under conditions of hypercapnia. The effects of DOI were found to be dose dependent, and the recovery of activity could be maintained for up to 2 h after a single injection. DOI-induced recovery was attenuated by the 5-HT2-receptor antagonist ketanserin but not with the 5-HT2C-receptor antagonist RS-102221, suggesting that 5-HT2A and not necessarily 5-HT2C receptors may be involved in the induction of respiratory recovery after cervical spinal cord injury.

4:40104. Experimental Distraction Spinal Cord Injury and Recovery of Motor Function

2006 ◽  
Vol 6 (5) ◽  
pp. 51S ◽  
Author(s):  
Mary K. Nagai ◽  
Kirk Dabney ◽  
Marina Ehrenshteyn ◽  
Dianna Willis ◽  
Jeffery Twiss
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Cord Injury ◽  
Recovery Of Motor Function

scholarly journals Spinal Interneurons and Forelimb Plasticity after Incomplete Cervical Spinal Cord Injury in Adult Rats

2015 ◽  
Vol 32 (12) ◽  
pp. 893-907 ◽  
Author(s):  
Elisa Janine Gonzalez-Rothi ◽  
Angela M. Rombola ◽  
Celeste A. Rousseau ◽  
Lynne M. Mercier ◽  
Garrett M. Fitzpatrick ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cervical Spinal Cord ◽  
Cervical Spinal Cord Injury ◽  
Adult Rats ◽  
Spinal Interneurons ◽  
Cord Injury
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