Abstract 1861: Advancing bispecific antibody discovery using common light chain immunoglobulin humanized mouse

2021 ◽  
Author(s):  
Li Hui ◽  
Hui Lu ◽  
Yabo Zhang ◽  
Shuwen Huang ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Light Chain ◽  
Bispecific Antibody ◽  
Humanized Mouse ◽  
Antibody Discovery ◽  
Light Chain Immunoglobulin

scholarly journals Identification of an octamer-binding site in the mouse kappa light-chain immunoglobulin enhancer.

1989 ◽  
Vol 9 (10) ◽  
pp. 4239-4247 ◽  
Author(s):  
R A Currie ◽  
R G Roeder
Keyword(s):  
B Cell ◽  
Binding Site ◽  
Light Chain ◽  
Specific Binding ◽  
Specific Factor ◽  
Immunoglobulin Gene ◽  
Kappa Light Chain ◽  
Nf Kappa B ◽  
Light Chain Immunoglobulin ◽  
A Site

A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specific (OTF-2) and ubiquitous (OTF-1) octamer-binding transcription factors specifically bound to a site centered about 136 bp 5' of the nuclear factor NF-kappa B site. A third specific factor, NF-kappa E, bound to a site that was about 75 bp 5' of the NF-kappa B site and within a region important for enhancer function. This novel factor was found in both mature B and HeLa cell nuclei. B-cell OTF-2, B-cell OTF-1, and HeLa OTF-1 bound to the kappa enhancer and kappa promoter octamer sites with similar affinities despite a 2-bp difference in the kappa enhancer octamer sequence. However, DNase I footprint analyses indicated that affinity-purified OTF-2 bound both to the enhancer OTF site and, surprisingly, to 80 bp of A + T-rich flanking sequence. Moreover, methylation interference studies demonstrated distinct differences in OTF interactions between the consensus octamer in the kappa promoter and the nonconsensus octamer identified in the enhancer. This novel observation of an OTF-binding site in the kappa enhancer provides a common link with the OTF sites in the promoter-proximal regions of all kappa promoters and thus mirrors the structural arrangement of OTF sites found in the promoters and enhancers of immunoglobulin heavy-chain genes.

scholarly journals Single-step Protein A and Protein G avidity purification methods to support bispecific antibody discovery and development

mAbs ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1464-1478 ◽  
Author(s):  
Romain Ollier ◽  
Paul Wassmann ◽  
Thierry Monney ◽  
Christelle Ries Fecourt ◽  
Sunitha Gn ◽  
...  
Keyword(s):  
Bispecific Antibody ◽  
Protein A ◽  
Single Step ◽  
Protein G ◽  
Antibody Discovery ◽  
Purification Methods

Characterization of light chain immunoglobulin in urine from animals and humans infected with prion diseases

2005 ◽  
Vol 162 (1-2) ◽  
pp. 12-18 ◽  
Author(s):  
Zehavit Kariv-Inbal ◽  
Michele Halimi ◽  
Yael Dayan ◽  
Roni Engelstein ◽  
Ruth Gabizon
Keyword(s):  
Light Chain ◽  
Prion Diseases ◽  
Light Chain Immunoglobulin

Identification of an octamer-binding site in the mouse kappa light-chain immunoglobulin enhancer

1989 ◽  
Vol 9 (10) ◽  
pp. 4239-4247
Author(s):  
R A Currie ◽  
R G Roeder
Keyword(s):  
B Cell ◽  
Binding Site ◽  
Light Chain ◽  
Specific Binding ◽  
Specific Factor ◽  
Immunoglobulin Gene ◽  
Kappa Light Chain ◽  
Nf Kappa B ◽  
Light Chain Immunoglobulin ◽  
A Site

A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specific (OTF-2) and ubiquitous (OTF-1) octamer-binding transcription factors specifically bound to a site centered about 136 bp 5' of the nuclear factor NF-kappa B site. A third specific factor, NF-kappa E, bound to a site that was about 75 bp 5' of the NF-kappa B site and within a region important for enhancer function. This novel factor was found in both mature B and HeLa cell nuclei. B-cell OTF-2, B-cell OTF-1, and HeLa OTF-1 bound to the kappa enhancer and kappa promoter octamer sites with similar affinities despite a 2-bp difference in the kappa enhancer octamer sequence. However, DNase I footprint analyses indicated that affinity-purified OTF-2 bound both to the enhancer OTF site and, surprisingly, to 80 bp of A + T-rich flanking sequence. Moreover, methylation interference studies demonstrated distinct differences in OTF interactions between the consensus octamer in the kappa promoter and the nonconsensus octamer identified in the enhancer. This novel observation of an OTF-binding site in the kappa enhancer provides a common link with the OTF sites in the promoter-proximal regions of all kappa promoters and thus mirrors the structural arrangement of OTF sites found in the promoters and enhancers of immunoglobulin heavy-chain genes.

Secondary monoclonal gammopathy after bone marrow autotransplantation as a cause of worse renal function in light chain immunoglobulin deposition disease

2016 ◽  
Vol 88 (6) ◽  
pp. 80
Author(s):  
I. G. Rekhtina ◽  
L. P. Mendeleeva ◽  
E. S. Stolyarevich ◽  
I. V. Galtseva ◽  
P. E. Povilaitite ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Renal Function ◽  
Light Chain ◽  
Monoclonal Gammopathy ◽  
Deposition Disease ◽  
Light Chain Immunoglobulin

scholarly journals Peripheral Neuropathy and VIth Nerve Palsy Related to Randall Disease Successfully Treated by High-Dose Melphalan, Autologous Blood Stem Cell Transplantation, and VIth Nerve Decompression Surgery

2010 ◽  
Vol 2010 ◽  
pp. 1-3
Author(s):  
C. Foguem ◽  
P. Manckoundia ◽  
P. Pfitzenmeyer ◽  
J.-L. Dupond
Keyword(s):  
Peripheral Neuropathy ◽  
Light Chain ◽  
Nerve Palsy ◽  
Motor Nerve ◽  
High Dose ◽  
Decompression Surgery ◽  
Kappa Light Chain ◽  
Light Chain Immunoglobulin ◽  
High Dose Melphalan ◽  
Nerve Decompression Surgery

Randall disease is an unusual cause of extraocular motor nerve (VI) palsy. A 35-year-old woman was hospitalized for sicca syndrome. The physical examination showed general weakness, weight loss, diplopia related to a left VIth nerve palsy, hypertrophy of the submandibular salivary glands, and peripheral neuropathy. The biological screening revealed renal insufficiency, serum monoclonal kappa light chain immunoglobulin, urinary monoclonal kappa light chain immunoglobulin, albuminuria, and Bence-Jones proteinuria. Bone marrow biopsy revealed medullar plasma cell infiltration. Immunofixation associated with electron microscopy analysis of the salivary glands showed deposits of kappa light chains. Randall disease was diagnosed. The patient received high-dose melphalan followed by autostem cell transplantation which led to rapid remission. Indeed, at the 2-month followup assessment, the submandibular salivary gland hypertrophy and renal insufficiency had disappeared, and the peripheral neuropathy, proteinuria, and serum monoclonal light chain had decreased significantly. The persistent diplopia was treated with nerve decompression surgery of the left extraocular motor nerve. Cranial nerve complications of Randall disease deserve to be recognized.

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