Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag- and CMA3-staining, and fluorescent in situ hybridization

1996 ◽  
Vol 75 (4) ◽  
pp. 234-239 ◽  
Author(s):  
J. Castro ◽  
A. Viñas ◽  
L. Sánchez ◽  
P. Martínez
Keyword(s):  
In Situ Hybridization ◽  
Brown Trout ◽  
Fluorescent In Situ Hybridization ◽  
Salmo Trutta ◽  
Brown Trout Salmo Trutta

Isolation, characterization, and chromosomal location of the tRNAMet genes in Atlantic salmon (Salmo salar) and brown trout (Salmo trutta)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 185-190 ◽  
Author(s):  
J Perez ◽  
P Moran ◽  
E Garcia-Vazquez
Keyword(s):  
In Situ Hybridization ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Genomic Library ◽  
Metaphase Chromosomes ◽  
Atlantic Salmon Salmo Salar ◽  
Brown Trout Salmo Trutta

This work describes the isolation, characterization, and physical location of the methionine tRNA in the genome of Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.). An Atlantic salmon genomic library was screened using a tRNAMet probe from Xenopus laevis. Two cosmid clones containing the Atlantic salmon tRNAMet gene were isolated, subcloned and sequenced. The tRNAMet was mapped to metaphase chromosomes by fluorescence in situ hybridization (FISH). Chromosomal data indicated that the tDNA of methionine is tandemly repeated in a single locus in both species. Analysis of genomic DNA by Southern hybridization confirmed the tandem organization of this gene. Key words: cosmids, cloning, in situ hybridization, tRNAMet.

Localization of the repetitive telomeric sequence (TTAGGG)n in four salmonid species

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1035-1038 ◽  
Author(s):  
M. Abuín ◽  
P. Martínez ◽  
L. Sánchez
Keyword(s):  
In Situ Hybridization ◽  
Salmo Salar ◽  
Fluorescent In Situ Hybridization ◽  
Salmo Trutta ◽  
Chromosome Region ◽  
Telomeric Sequence ◽  
Evolutionary Mechanism ◽  
Salmonid Species ◽  
Telomeric Probe

We have analyzed the localization of the highly conserved telomeric sequence (TTAGGG)n in four salmonid species, two of the genus Salmo (Salmo trutta and Salmo salar) and two of the genus Oncorhynchus (Onchorhynchus mykiss and Onchorhynchus kisutch), by fluorescent in situ hybridization. As expected, the hybridization signal was mostly localized at the telomeres of all chromosomes in the four species. Two species evidenced special hybridization sites with the telomeric probe: (i) interstitial heterochromatic blocks in particular long chromosomes in S. salar, this observation supports tandem fusions as the karyotypic evolutionary mechanism leading to the formation of the long acrocentric and submetacentric chromosomes in the karyotype of S. salar; (ii) the whole NOR region in O. mykiss; this observation suggests that the (TTAGGG)n sequence is scattered all along this chromosome region. Key words : Salmo, Oncorhynchus, telomeres, in situ hybridization, evolution.

Sign in / Sign up

Export Citation Format

Share Document