Idiopathic Acquired Generalized Anhidrosis: Electron-Microscopic and Immunohistochemical Studies and Analysis of Lectin-Binding Pattern of the Cell Membrane

Dermatology ◽  
1989 ◽  
Vol 178 (3) ◽  
pp. 123-125 ◽  
Author(s):  
Tadashi Terui ◽  
Yohkon Ohkawa ◽  
Hachiro Tagami
Author(s):  
R.P. Nayyar ◽  
C.F. Lange ◽  
J. L. Borke

Streptococcal cell membrane (SCM) antiserum injected mice show a significant thickening of glomerular basement membrane (GBM) and an increase in mesangial matrix within 4 to 24 hours of antiserum administration (1,2,3). This study was undertaken to evaluate the incorporation of 3H proline into glomerular cells and GBM under normal and anti-SCM induced conditions. Mice were administered, intraperitoneally, 0.1 ml of normal or anti-SCM serum followed by a 10 µC/g body weight injection of 3H proline. Details of the preparation of anti-SCM (Group A type 12 streptococcal pyogenes) and other sera and injection protocol have been described elsewhere (2). After 15 minutes of isotope injection a chase of cold proline was given and animal sacrificed at 20 minutes, 1,2,4,8,24 and 48 hours. One of the removed kidneys was processed for immunofluorescence, light and electron microscopic radioautographic studies; second kidney was used for GBM isolation and aminoacid analysis.


2013 ◽  
Vol 48 (5) ◽  
pp. 850-857 ◽  
Author(s):  
CG Barbeito ◽  
HH Ortega ◽  
V Matiller ◽  
EJ Gimeno ◽  
NR Salvetti

2012 ◽  
Vol 75 (8) ◽  
pp. 1124-1135 ◽  
Author(s):  
Gianluca Accogli ◽  
Sara Zizza ◽  
Angel García-lópez ◽  
Carmen Sarasquete ◽  
Salvatore Desantis

1967 ◽  
Vol 2 (1) ◽  
pp. 71-76
Author(s):  
N. MEISELMAN ◽  
A. KOHN ◽  
D. DANON

Treatment of FL or Lu 106 epithelial cells with Newcastle disease virus (NDV) at an input multiplicity of 500 EID50 per cell induces in these cells the formation of polykaryocytes at the end of 2-3 h of contact. Electron micrographs of such NDV-treated monolayers after 2-10 min of incubation show the presence of virions adsorbed to the cell membranes, in vacuoles and with the viral envelope partly fused with the cell membrane. In polykaryocytes induced by NDV, remnants of cell membranes showing numerous breaks may still be present after 3 h.


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