Electron Microscopic Study of Penetration of Newcastle Disease Virus into Cells Leading to Formation of Polykaryocytes

1967 ◽  
Vol 2 (1) ◽  
pp. 71-76
Author(s):  
N. MEISELMAN ◽  
A. KOHN ◽  
D. DANON
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Newcastle Disease Virus ◽  
Microscopic Study ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cell Membranes ◽  
Viral Envelope ◽  
Electron Microscopic ◽  
Input Multiplicity

Treatment of FL or Lu 106 epithelial cells with Newcastle disease virus (NDV) at an input multiplicity of 500 EID50 per cell induces in these cells the formation of polykaryocytes at the end of 2-3 h of contact. Electron micrographs of such NDV-treated monolayers after 2-10 min of incubation show the presence of virions adsorbed to the cell membranes, in vacuoles and with the viral envelope partly fused with the cell membrane. In polykaryocytes induced by NDV, remnants of cell membranes showing numerous breaks may still be present after 3 h.

scholarly journals The Tween 80 Toxicity in Chicken Embryos and Effect on the Kinetics of Newcastle Disease Virus Replication

2020 ◽  
Vol 14 (4) ◽  
pp. 229-236
Author(s):  
Shahla Shahsavandi ◽  
◽  
Mohammad Majid Ebrahimi ◽  
Mohammad Bagher Ghadiri ◽  
Mohammad Reza Samiee ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Tween 80 ◽  
Cell Membrane Permeability ◽  
Ionic Surfactant ◽  
Infectious Dose ◽  
Chicken Embryos ◽  
High Concentrations

Background: Non-ionic surfactant, Tween-80 (TW80) is commonly used for drug delivery due to its effect on the cell membrane permeation. The change in permeability can also increase viral infectivity in cells. This study was undertaken to improve upon Newcastle disease virus (NDV) titer cultivated with embryonic chicken eggs.  Methods: The toxicity of TW80 was investigated against chicken embryos at varying concentrations, and changes in the morphology and weights of the heart, liver, and spleen of 4-day old chicken embryos were analyzed. Also, the effect of non-toxic concentrations of TW80 was examined on the infectivity of NDV. The virus was titrated in the allantoic fluid, using a 50% embryo infectious dose (EID50).  Results: At high concentrations of TW80, hemorrhage-induced mortality was observed in embryos at the early stages of incubation. The embryos’ viability was not affected at low TW80 concentrations, indicating that its toxicity to the chicken embryos was dose-dependent. The infectivity titer of NDV was increased in the presence of TW80 compared to those inoculated with NDV only.  Conclusion: The data suggest that TW80 is toxic to chicken embryos at high concentrations, but it enhances cell membrane permeability for NDV particles at low concentrations without affecting the embryos’ viability.

Protective effect of antibodies to two viral envelope glycoproteins on lethal infection with Newcastle disease virus

1987 ◽  
Vol 94 (1-2) ◽  
pp. 97-107 ◽  
Author(s):  
Y. Umino ◽  
T. Kohama ◽  
M. Kohase ◽  
A. Sugiura ◽  
H. -D. Klenk ◽  
...  
Keyword(s):  
Protective Effect ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Viral Envelope ◽  
Envelope Glycoproteins ◽  
Lethal Infection
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