scholarly journals Non-Invasive Prenatal RHD Genotyping Using Cell-Free Fetal DNA from Maternal Plasma: An Italian Experience

2014 ◽  
Vol 42 (1) ◽  
pp. 22-28 ◽  
Author(s):  
Elena Picchiassi ◽  
Gian Carlo Di Renzo ◽  
Federica Tarquini ◽  
Vittorio Bini ◽  
Michela Centra ◽  
...  
Keyword(s):  
Maternal Plasma ◽  
Non Invasive ◽  
Fetal Dna ◽  
Italian Experience ◽  
Cell Free Fetal Dna

Non-invasive prenatal diagnosis using cell-free fetal DNA in maternal plasma from PGD pregnancies

2009 ◽  
Vol 19 (5) ◽  
pp. 714-720 ◽  
Author(s):  
Ying Li ◽  
Gheona Altarescu ◽  
Paul Renbaum ◽  
Talia Eldar-Geva ◽  
Ephrat Levy-Lahad ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Maternal Plasma ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Application of real-time PCR of sex-independent insertion-deletion polymorphisms to determine fetal sex using cell-free fetal DNA from maternal plasma

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Sherry Sze Yee Ho ◽  
Angela Barrett ◽  
Henna Thadani ◽  
Cecille Laureano Asibal ◽  
Evelyn Siew-Chuan Koay ◽  
...  
Keyword(s):  
Real Time ◽  
Y Chromosome ◽  
Real Time Pcr ◽  
Genetic Material ◽  
Maternal Plasma ◽  
Fetal Sex ◽  
Female Fetus ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

AbstractPrenatal diagnosis of sex-linked disorders requires invasive procedures, carrying a risk of miscarriage of up to 1%. Cell-free fetal DNA (cffDNA) present in cell-free DNA (cfDNA) from maternal plasma offers a non-invasive source of fetal genetic material for analysis. Detection of Y-chromosome sequences in cfDNA indicates presence of a male fetus; in the absence of a Y-chromosome signal a female fetus is inferred. We aimed to validate the clinical utility of insertion-deletion polymorphisms (INDELs) to confirm presence of a female fetus using cffDNA.Quantitative real-time PCR (qPCR) for the Y-chromosome-specific sequence,Fetal sex was correctly determined in 77/82 (93.9%) cfDNA samples.We have validated a non-invasive prenatal test to confirm fetal sex as early as 6 gestational weeks using cffDNA from maternal plasma.

scholarly journals Analysis of Cell-free Fetal DNA in Plasma and Serum of Pregnant Women

2005 ◽  
Vol 53 (3) ◽  
pp. 297-299 ◽  
Author(s):  
T.V. Zolotukhina ◽  
N.V. Shilova ◽  
E. Yu Voskoboeva
Keyword(s):  
Pregnant Women ◽  
Maternal Plasma ◽  
Sry Gene ◽  
Male Fetus ◽  
Non Invasive ◽  
Fetal Dna ◽  
Fetal Gender ◽  
Cell Free Fetal Dna ◽  
Invasive Method

Sixty blood samples from pregnant women during gestational weeks 9–28 were investigated. Cell-free fetal DNA was extracted from maternal plasma or serum to be detected by nested PCR for determination of fetal gender. The SRY gene as a marker for fetal Y chromosome was detected in 34/36 women carrying a male fetus. In 3/24 women carrying female fetuses, the SRY sequence was also detected. Overall, fetal sex was correctly predicted in 91.7% of the cases. Therefore, the new, non-invasive method of prenatal diagnosis of fetal gender for women at risk of producing children with X-linked disorders is reliable, secure, and can substantially reduce invasive prenatal tests.

Non-invasive prenatal determination of fetal gender using QF-PCR analysis of cell-free fetal DNA in maternal plasma

2012 ◽  
Vol 413 (5-6) ◽  
pp. 600-604 ◽  
Author(s):  
Shin Young Kim ◽  
Ji Hyae Lim ◽  
So Yeon Park ◽  
Moon Young Kim ◽  
June Seek Choi ◽  
...  
Keyword(s):  
Maternal Plasma ◽  
Pcr Analysis ◽  
Non Invasive ◽  
Fetal Dna ◽  
Fetal Gender ◽  
Cell Free Fetal Dna

scholarly journals Development of an Automated System for the Analysis of Cell-Free Fetal DNA from Maternal Plasma for Non-Invasive Pre-Natal Diagnostics

Proceedings ◽  
2017 ◽  
Vol 1 (8) ◽  
pp. 802
Author(s):  
Josep Lluís Acero ◽  
Carmen Bermudo ◽  
Teresa Mairal ◽  
Ciara O’Sullivan ◽  
Ian Riley ◽  
...  
Keyword(s):  
Maternal Plasma ◽  
Automated System ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

The analysis of circulating cell-free (cf) DNA from plasma, serum or urine, has the potential to [...]

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