Butanol Extraction of Serum and Urinary ?-Glutamyltransferase

2015 ◽  
pp. 137-139
Author(s):  
Peter R. Beck
Keyword(s):  
Butanol Extraction

False-positive reactions for porphobilinogen. Detection by butanol extraction and report of a case

JAMA ◽  
1966 ◽  
Vol 197 (7) ◽  
pp. 584-586
Author(s):  
M. H. Fine
Keyword(s):  
False Positive ◽  
False Positive Reactions ◽  
Butanol Extraction

scholarly journals A Modified Kulka Micromethod for the Rapid and Safe Analysis of Fructose and 1-Deoxy-d-xylulose-5-phosphate

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 77
Author(s):  
Shreya Shaw ◽  
Robin Ghosh
Keyword(s):  
High Throughput ◽  
Enzyme Assays ◽  
Original Formulation ◽  
Laboratory Environment ◽  
Butanol Extraction ◽  
Common Substrate ◽  
Biology Laboratory ◽  
Resorcinol Method ◽  
Growth Experiments

The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542–548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology laboratory environment; and (b) in its original formulation, it is unsuited for modern high-throughput applications. Here, we have developed a modified Kulka assay, which contains a safer formulation, employing approx. 5.4 M HCl in 250 µL aliquots, and is suitable for use in high-throughput systems biology or enzymatic applications. The modified assay has been tested extensively for the measurement of two ketoses—fructose (a common substrate in cell growth experiments) and 1-deoxy-d-xylulose-5-phosphate (DXP), the product of the DXP-synthase reaction—which until now has only been assayable using time-consuming chromatographic methods or radioactivity. The Kulka microassay has a sensitivity of 0–250 nmol fructose or 0–500 nmol DXP. The assay is suitable for monitoring the consumption of fructose in bacterial growth experiments but is too insensitive to be used directly for the measurement of DXP in in vitro enzyme assays. However, we show that after concentration of the DXP-enzyme mix by butanol extraction, the Kulka resorcinol method can be used for enzyme assays.

New ionic liquid [P4,4,4,4][NTf2] in bio-butanol extraction on investigation of limiting activity coefficients

2018 ◽  
Vol 475 ◽  
pp. 89-94 ◽  
Author(s):  
Urszula Domańska ◽  
Michal Wlazło ◽  
Monika Karpińska ◽  
Maciej Zawadzki
Keyword(s):  
Ionic Liquid ◽  
Activity Coefficients ◽  
Butanol Extraction

EFFECT OF GLUCOCORTICOIDS, BUTANOL EXTRACTION OF SERUM AND ADRENALECTOMY ON SOMATOMEDIN LEVELS IN THE RAT

1981 ◽  
Vol 90 (3) ◽  
pp. 411-420 ◽  
Author(s):  
A. T. HOLDER ◽  
M. A. PREECE ◽  
M. WALLIS
Keyword(s):  
Inhibitory Effect ◽  
Rat Serum ◽  
Immature Rat ◽  
Cartilage Metabolism ◽  
Butanol Extraction ◽  
Adrenalectomized Rats

Serum from adrenalectomized rats was equipotent with serum from non-adrenalectomized animals when measured in a rat cartilage somatomedin bioassay. Extraction with butanol of sera from normal or adrenalectomized rats reduced their potency in the somatomedin bioassay rather than increasing it as has been previously reported. Butanol-soluble inhibitors of cartilage metabolism were found in sera from both normal and adrenalectomized rats. Cortisol and corticosterone, up to mildly supraphysiological levels, were found to have no effect on basal cartilage metabolism. These results suggest that physiological levels of glucocorticoids do not exert an inhibitory effect on the uptake of 35SO42− into immature rat cartilage. Since butanol-soluble inhibitors of cartilage metabolism were found in adrenalectomized rat serum it is unlikely that these substances are glucocorticoids.

Gas Chromatographic Determination of Lower Alcohols in Biologic Samples

1964 ◽  
Vol 10 (5) ◽  
pp. 429-432 ◽  
Author(s):  
Harold Lyons ◽  
Jacquelyn Bard
Keyword(s):  
Simultaneous Determination ◽  
Calcium Sulfate ◽  
Low Cost ◽  
Chromatographic Determination ◽  
Good Sensitivity ◽  
Gas Chromatograph ◽  
Butanol Extraction ◽  
Hydrogen Flame ◽  
Flame Detection

Abstract A procedure is described for the simultaneous determination of methanol, ethanol, and isopropanol in biologic samples, using a low-cost gas chromatograph. A combination of the use of anhydrous calcium sulfate (Drierite) absorption of most of the water in the sample followed by an n-butanol extraction of the alcohols from the solid absorbent is employed. This extraction technic is efficient and affords good sensitivity while eliminating the need for hydrogen flame detection and sample-port clean-out.

Butanol extraction to predict bioavailability of PAHs in soil

Chemosphere ◽  
2002 ◽  
Vol 46 (7) ◽  
pp. 1011-1017 ◽  
Author(s):  
Hans-Holger Liste ◽  
Martin Alexander
Keyword(s):  
Butanol Extraction
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