Pharmaceutical medicines play an important role in human life that helps to cure different diseases. For the chemical and
pharmaceutical analysis of the drug effective quality control and pharmacodynamic and pharmacokinetic studies are needed. Several
methods have been developed and validated for its pharmaceutical and biological materials since it was introduced as an important
antiretroviral agent. The literature survey reveals that only four RP HPLC method and one HPTLC was developed for the simultaneous
estimation of Dolutegravir, Lamivudine and Tenofovir disoproxil fumarate in tablet dosage form. These three drugs are used as
antiretroviral medicines which are used for HIV or AIDS prevention and treatment. The goal of this review is to define and establish a
simple, precise and selective method for estimating the dosage of Lamivudine, Tenofovir Disoproxil fumarate and Dolutegravir in
biological and pharmaceutical dosage form using the HPLC, HPTLC, UPLC, UV Visible spectroscopy, LC/MS, Infrared spectroscopy,
NMR spectroscopy, Microbiological assay, Electrochemical studies and Capillary electrophoresis. UV-detector HPLC is commonly used
in pharmaceuticals and LC-MS are widely used for biological materials with mass and tandem mass spectrometer detector systems.
Various parameters such as device suitability, process accuracy, precision, linearity, detection limit will validate the UV Visible
spectroscopy and RP-HPLC technique.