Identifikasi Sakarin pada Es Kelapa Muda yang Dijual di Pasar Rajawali Kota Palangka Raya

Saccharin is a class of food additives permitted by Indonesia Republic Minister of Health Regulation No. 33 of 2012 for use in drinks, but if consumed in excess can cause harmful effects on health such as causing allergies and bladder cancer. The purpose of this study was to determine the presence of saccharin in coconut ice drinks in Pasar Rajawali Kota Palangka Raya. The sample used was 5 samples and the sampling technique was saturated sampling. Saccharin examination is done qualitatively using the resorcinol method. The results of research that has been done show that all samples of coconut ice drinks that have been tested negatively contain saccharin sweeteners.

A Modified Kulka Micromethod for the Rapid and Safe Analysis of Fructose and 1-Deoxy-d-xylulose-5-phosphate

The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542–548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology laboratory environment; and (b) in its original formulation, it is unsuited for modern high-throughput applications. Here, we have developed a modified Kulka assay, which contains a safer formulation, employing approx. 5.4 M HCl in 250 µL aliquots, and is suitable for use in high-throughput systems biology or enzymatic applications. The modified assay has been tested extensively for the measurement of two ketoses—fructose (a common substrate in cell growth experiments) and 1-deoxy-d-xylulose-5-phosphate (DXP), the product of the DXP-synthase reaction—which until now has only been assayable using time-consuming chromatographic methods or radioactivity. The Kulka microassay has a sensitivity of 0–250 nmol fructose or 0–500 nmol DXP. The assay is suitable for monitoring the consumption of fructose in bacterial growth experiments but is too insensitive to be used directly for the measurement of DXP in in vitro enzyme assays. However, we show that after concentration of the DXP-enzyme mix by butanol extraction, the Kulka resorcinol method can be used for enzyme assays.

The unique giant Irish moss (Chondrus crispus) from Basin Head: health assessment in relation to reference sites on Prince Edward Island

Irish moss (Chondrus crispus Stackhouse) is a red alga that is common in Atlantic Canada. A unique strain of this species, the giant Irish moss, grows in a single location and is under strict protection. Unlike the common coastal form, the giant Irish moss reproduces solely by fragmentation and is found in gametophyte form. A 99.9% decline in giant Irish moss abundance (1999–2012) prompted this study to address two questions: whether the giant Irish moss remains 100% vegetative and gametophytic, and whether such decline is related to factors leading to a reduction in photosynthetic health. Six populations of the common Irish moss strain were compared with two populations of giant Irish moss, and their life history phases determined using a resorcinol method. The common Irish moss populations exhibited a 65%–86% ratio of gametophytic:tetrasporophytic fronds, while both giant strain populations were 100% gametophytic. Photosynthetic efficiency was measured with Pulse-Amplitude-Modulation (PAM) fluorometry and neither giant moss population had significantly different quantum yield values from the littoral populations. Hence, these analyses provided no evidence of giant Irish moss being exposed to particular sources of stress linked to water or habitat quality and alternative factors explaining the decline of this unique strain are proposed.

The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin

Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva.

Automated Method for Determination of Bound N-Acetylneuraminic Acid in Serum

A modified resorcinol method for sialic acid determination has been successfully adapted to the Technicon AutoAnalyzer. The present method requires only 25 µl of serum, and 30 samples can be analyzed per hour. It may be used to measure sialic acid in concentrations ranging from 10 to 100 mg/liter.