Divergence between C. melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern

2016 ◽  
Vol 150 (2) ◽  
pp. 150-155 ◽  
Author(s):  
Kunpeng Li ◽  
Huaisong Wang ◽  
Jiming Wang ◽  
Jianying Sun ◽  
Zongyun Li ◽  
...  
Keyword(s):  
Diploid Species ◽  
Distribution Patterns ◽  
45S Rdna ◽  
Rdna Sites ◽  
Cucumis Species ◽  
Cytogenetic Evidence ◽  
Rdna Fish ◽  
5S And 45S Rdna ◽  
Painting Probes

The 5S and 45S rDNA sites are useful chromosome landmarks and can provide valuable information about karyotype evolution and species interrelationships. In this study, we employed fluorescence in situ hybridization (FISH) to determine the number and chromosomal location of 5S and 45S rDNA loci in 8 diploid Cucumis species. Two oligonucleotide painting probes specific for the rDNA-bearing chromosomes in C. melo were hybridized to other Cucumis species in order to investigate the homeologies among the rDNA-carrying chromosomes in Cucumis species. The analyzed diploid species showed 3 types of rDNA distribution patterns, which provided clear cytogenetic evidence on the divergence between C. melo and wild diploid African Cucumis species. The present results not only show species interrelationships in the genus Cucumis, but the rDNA FISH patterns can also be used as cytological markers for the discrimination of closely related species. The data will be helpful for breeders to choose the most suitable species from various wild species for improvement of cultivated melon.

Comparative analysis of rDNA distribution in 29 species of Begonia sect. Coelocentrum Irmsch.

Phytotaxa ◽  
2018 ◽  
Vol 381 (1) ◽  
pp. 141 ◽  
Author(s):  
YAN-LI HAN ◽  
DAI-KE TIAN ◽  
NAI-FENG FU ◽  
YAN XIAO ◽  
ZONG-YUN LI ◽  
...  
Keyword(s):  
5S Rdna ◽  
Distribution Patterns ◽  
Hybrid Origin ◽  
45S Rdna ◽  
Species Relationships ◽  
Rdna Sites ◽  
Rdna Fish ◽  
5S And 45S Rdna ◽  
Chromosome Landmarks

The rDNA sites are useful chromosome landmarks and can provide valuable information for species identification and species relationships. In this study, we investigated the distribution of 5S and 45S rDNA sites in 29 species of Begonia sect. Coelocentrum Irmsch. using a two-colour fluorescence in situ hybridization (FISH) technique. This is the first report of chromosomal rDNA mapping in Begonia species. The analyzed species showed considerable diversity in rDNA distribution patterns. The 45S rDNA signals are always located in terminal regions on 1−4 chromosomes, while 5S rDNA signals are mainly located at proximal regions on 2−8 chromosomes, varying from specific major signals to highly dispersed minor signals. Based on rDNA FISH patterns, most of the investigated species could be distinguished from each other and species relationships were identified. In addition, the results provided clear proof that B. huangii is of hybrid origin and the triploid B. longgangensis was allotriploid rather than autotriploid as suggested before. The data will provide a useful reference for evaluation, conservation and utilization of the natural resources of the mega-diverse genus Begonia.

scholarly journals Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)

2003 ◽  
Vol 128 (5) ◽  
pp. 736-740 ◽  
Author(s):  
Young A Choi ◽  
Ryutaro Tao ◽  
Keizo Yonemori ◽  
Akira Sugiura
Keyword(s):  
Fluorescent In Situ Hybridization ◽  
Diploid Species ◽  
5S Rdna ◽  
Diospyros Kaki ◽  
45S Rdna ◽  
Multicolor Fish ◽  
Rdna Probe ◽  
Rdna Sites ◽  
5S And 45S Rdna

5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on different chromosomes of Diospyros species tested, suggesting that 5S and 45S rDNA sites can be used as chromosome markers in Diospyros. The number of 5S rDNA sites varied with the Diospyros species. More 5S rDNA sites were observed in four diploid species native to Southern Africa than in three Asian diploid species. The former had four or six 5S rDNA sites while the latter had two. Three Asian polyploidy species had four to eight 5S rDNA sites. Among the Asian species, the number of 5S rDNA sites seemed to increase according to ploidy level of species. These features of 5S rDNA sites were very similar to those of 45S rDNA sites in Diospyros. Phylogenetic relationship between D. kaki and wild species tested are discussed based on the number and chromosomal distribution of 5S and 45S rDNA.

Phylogenetic study of Ipomoeeae (Convolvulaceae) based on chromosome painting

2020 ◽  
Author(s):  
Lei Chen ◽  
Jianying Sun ◽  
Dan Su ◽  
Qinghe Cao ◽  
Zongyun Li ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Chromosome Painting ◽  
Distribution Patterns ◽  
The Other ◽  
Phylogenetic Study ◽  
45S Rdna ◽  
Species Relationship ◽  
5S And 45S Rdna ◽  
The Difference

Abstract Background: The phylogenetic relationships for Ipomoeeae species are incongruence in previous studies. Comparative chromosome painting (CCP) can provide valuable information for phylogenetic relationships among species. Results: In this study, 31 Ipomoea species were analyzed by oligo-based chromosome painting (CP) technique to better understand their phylogenetic relationships. The results indicated that the distribution patterns of oligo probes in three species with 28 chromosomes were significantly different from the other species with 30 chromosomes. Therefore, we proposed that the difference of chromosome number should be foremost factor in determining the phylogenetic relationships among species and the previous division of two large clades in Ipomoeeae wasn’t supported. The division of smaller clades within the Astripomoeinae in Ipomoeeae was generally supported with a few exceptions, whereas the division of smaller clades within Argyreiinae wasn’t resolved by our CP results. In addition, species relationship for the species belonging to same smaller clade with same CP pattern was further resolved by physical mapping of the 5S and 45S rDNA using fluorescence in situ hybridization (FISH) technique. Conclusions: This study is the first phylogenetic analysis of Ipomoea species based on CP technique. Our results provide intuitive and reliable resolution of phylogenetic relationships for 31 Ipomoea species and demonstrate the validity of oligo-based CCP analysis in phylogenetic study.

Cytogenetic evidence of nucleolar dominance in allotetraploid species of Brachypodium

Genome ◽  
2008 ◽  
Vol 51 (5) ◽  
pp. 387-391 ◽  
Author(s):  
Dominika Idziak ◽  
Robert Hasterok
Keyword(s):  
Nuclear Dna ◽  
Genomic Library ◽  
Diploid Species ◽  
Rrna Genes ◽  
Root Tip ◽  
45S Rdna ◽  
Bac Clone ◽  
Nucleolar Dominance ◽  
Rdna Sites ◽  
Cytogenetic Evidence

Sequential silver staining and fluorescence in situ hybridization (FISH) were used to establish activity and number of 45S rDNA sites in meristematic root tip cells of 6 ecotypes of allotetraploid (2n = 4x = 30) species of Brachypodium and their putative ancestors, B. distachyon (2n = 2x = 10) and ABR114 (2n = 2x = 20). Using either total nuclear DNA of ABR114 or the ABR1-63-E6 BAC clone from a B. distachyon genomic library as an auxiliary probe, it was possible to distinguish by FISH between the two genomes composing the ecotypes of allotetraploid Brachypodium species and to determine unambiguously the parentage of both dominant and suppressed rRNA genes. Each of the diploid species possessed two rDNA loci, both transcriptionally active. The number of 45S rDNA sites in 6 ecotypes of allotetraploid Brachypodium species was always equal to the sum of loci present in their putative diploid parents. Two smaller sites were located in chromosomes corresponding to the ABR114 chromosomal set, and two larger ones in the chromosomes of B. distachyon origin. In all analyzed allotetraploid ecotypes, only rRNA genes belonging to the B. distachyon–like genome were transcriptionally active, while rDNA from the other parental genome was always suppressed. Thus the occurrence of nucleolar dominance in the allotetraploid (2n = 4x = 30) species of Brachypodium is demonstrated for the first time.

scholarly journals Comparative molecular cytogenetic characterization of five wild Vigna species (Fabaceae)

2020 ◽  
Vol 14 (2) ◽  
pp. 243-264
Author(s):  
Chao-Wen She ◽  
Ying Mao ◽  
Xiang-Hui Jiang ◽  
Chun-Ping He
Keyword(s):  
Genomic Dna ◽  
Interspecific Variation ◽  
Rrna Genes ◽  
Fluorochrome Banding ◽  
45S Rdna ◽  
Molecular Cytogenetic ◽  
Rdna Sites ◽  
Cytogenetic Characterization ◽  
Rdna Fish

To extend our knowledge on karyotype variation of the genus Vigna Savi, 1824, the chromosomal organization of rRNA genes and fluorochrome banding patterns of five wild Vigna species were studied. Sequential combined PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindole) (CPD) staining and fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes were used to analyze the karyotypes of V. luteola (Jacquin, 1771) Bentham, 1959, V. vexillata (Linnaeus, 1753) A. Richard, 1845, V. minima (Roxburgh, 1832) Ohwi & H. Ohashi, 1969, V. trilobata (Linnaeus, 1753) Verdcourt, 1968, and V. caracalla (Linnaeus, 1753) Verdcourt,1970. For further phylogenetic analysis, genomic in situ hybridization (GISH) with the genomic DNA of V. umbellata (Thunberg, 1794) Ohwi & H.Ohashi, 1969 onto the chromosomes of five wild Vigna species was also performed. Detailed karyotypes were established for the first time using chromosome measurements, fluorochrome bands, and rDNA-FISH signals. All species had chromosome number 2n = 2x = 22, and symmetrical karyotypes that composed of only metacentric or metacentric and submetacentric chromosomes. CPD staining revealed all 45S rDNA sites in the five species analyzed, (peri)centromeric GC-rich heterochromatin in V. luteola, V. trilobata and V. caracalla, interstitial GC-rich and pericentromeric AT-rich heterochromatin in V. caracalla. rDNA-FISH revealed two 5S loci in V. caracalla and one 5S locus in the other four species; one 45S locus in V. luteola and V. caracalla, two 45S loci in V. vexillata and V. trilobata, and five 45S loci in V. minima. The karyotypes of the studied species could be clearly distinguished by the karyotypic parameters, and the patterns of the fluorochrome bands and the rDNA sites, which revealed high interspecific variation among the five species. The V. umbellata genomic DNA probe produced weak signals in all proximal regions of V. luteola and all (peri)centromeric regions of V. trilobata. The combined data demonstrate that distinct genome differentiation has occurred among the five species during evolution. The phylogenetic relationships between the five wild species and related cultivated species of Vigna are discussed based on our present and previous molecular cytogenetic data.

Comparative Karyotype Investigations in the European Crayfish Astacus astacus and A. leptodactylus (Decapoda, Astacidae)

Crustaceana ◽  
2011 ◽  
Vol 84 (12-13) ◽  
pp. 1497-1510 ◽  
Author(s):  
M. Pavlica ◽  
M. Mcžić ◽  
G. Klobučar ◽  
M. Šrut ◽  
I. Maguire ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Chromosome Complement ◽  
Size Difference ◽  
45S Rdna ◽  
Astacus Astacus ◽  
Fluorochrome Staining ◽  
Rdna Sites ◽  
Freshwater Habitats ◽  
Acrocentric Chromosomes

AbstractThis study reports on the chromosome number and karyological characteristics of the endangered species of European crayfish, Astacus astacus and A. leptodactylus (Decapoda, Astacidae), both native to Croatian freshwater habitats. The karyotype of A. astacus and A. leptodactylus consists of 2n = 176 and 2n = 180 chromosomes, respectively. The haploid chromosome complement of A. astacus consists of 52 metacentric, 35 metacentric-submetacentric, and 1 acrocentric chromosomes. Fluorochrome staining with 4,6-diamino-2-phenylindole (DAPI) has revealed that the karyotypes of A. astacus and A. leptodactylus are characterized by large heterochromatic blocks located at centromeric and intercalary positions on the chromosomes. Interstitial heterochromatic blocks were more frequent in A. astacus than in A. leptodactylus. In both species pairing of chromosomes in meiosis was regular with the majority of bivalents in a ring- and a dumbbell-form. Fluorescence in situ hybridization (FISH) has revealed that two 45S rDNA loci were present in the investigated species. In A. astacus one of the two 45S rDNA-bearing chromosome pairs was highly heteromorphic, exhibiting a three-fold size difference between 45S rDNA sites on homologous chromosomes. Such a size difference was significantly less pronounced in A. leptodactylus. The karyotype differences between A. astacus and A. leptodactylus suggest changes in chromosome number as well as position of repetitive DNAs have played a role in the karyotype evolution of the species of Astacus.

scholarly journals A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH)

2009 ◽  
Vol 55 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Ke Bi ◽  
James P. Bogart ◽  
Jinzhong Fu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dispersal Ability ◽  
Habitat Isolation ◽  
45S Rdna ◽  
Isolated Populations ◽  
Ambystoma Jeffersonianum ◽  
Rdna Sites ◽  
Rdna Polymorphism

Abstract The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A. jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A. Jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution.

Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridisation

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 911-918 ◽  
Author(s):  
Ki-Byung Lim ◽  
Jannie Wennekes ◽  
J Hans de Jong ◽  
Evert Jacobsen ◽  
Jaap M van Tuyl
Keyword(s):  
In Situ Hybridisation ◽  
5S Rdna ◽  
Lilium Longiflorum ◽  
Fluorescence In Situ Hybridisation ◽  
45S Rdna ◽  
C Banding ◽  
Rdna Sequences ◽  
Rdna Signal ◽  
5S And 45S Rdna

Detailed karyotypes of Lilium longiflorum and L. rubellum were constructed on the basis of chromosome arm lengths, C-banding, AgNO3 staining, and PI-DAPI banding, together with fluorescence in situ hybridisation (FISH) with the 5S and 45S rDNA sequences as probes. The C-banding patterns that were obtained with the standard BSG technique revealed only few minor bands on heterologous positions of the L. longiflorum and L. rubellum chromosomes. FISH of the 5S and 45S rDNA probes on L. longiflorum metaphase complements showed overlapping signals at proximal positions of the short arms of chromosomes 4 and 7, a single 5S rDNA signal on the secondary constriction of chromosome 3, and one 45S rDNA signal adjacent to the 5S rDNA signal on the subdistal part of the long arm of chromosome 3. In L. rubellum, we observed co-localisation of the 5S and 45S rDNA sequences on the short arm of chromosomes 2 and 4 and on the long arms of chromosomes 2 and 3, and two adjacent bands on chromosome 12. Silver staining (Ag-NOR) of the nucleoli and NORs in L. longiflorum and L. rubellum yielded a highly variable number of signals in interphase nuclei and only a few faint silver deposits on the NORs of mitotic metaphase chromosomes. In preparations stained with PI and DAPI, we observed both red- and blue-fluorescing bands at different positions on the L. longiflorum and L. rubellum chromosomes. The red-fluorescing or so-called reverse PI-DAPI bands always coincided with rDNA sites, whereas the blue-fluorescing DAPI bands corresponded to C-bands. Based on these techniques, we could identify most of chromosomes of the L. longiflorum and L. rubellum karyotypes.Key words: fluorescence in situ hybridisation, FISH, 5S rDNA, 45S rDNA, C-banding, reverse PI-DAPI banding.

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution in Cucumis

Genome ◽  
2016 ◽  
Vol 59 (7) ◽  
pp. 449-457 ◽  
Author(s):  
Zhen-Tao Zhang ◽  
Shu-Qiong Yang ◽  
Zi-Ang Li ◽  
Yun-Xia Zhang ◽  
Yun-Zhu Wang ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
5S Rdna ◽  
Chromosome Analysis ◽  
45S Rdna ◽  
Evolutionary Patterns ◽  
Site Number ◽  
Rdna Sites ◽  
Wild Accessions ◽  
Ribosomal Dnas

Ribosomal DNAs are useful cytogenetic markers for chromosome analysis. Studies investigating site numbers and distributions of rDNAs have provided important information for elucidating genome organization and chromosomal relationships of many species by fluorescence in situ hybridization. But relevant studies are scarce for species of the genus Cucumis, especially in wild species. In the present study, FISH was conducted to investigate the organization of 45S and 5S rDNA among 20 Cucumis accessions, including cultivars and wild accessions. Our results showed that the number of 45S rDNA sites varied from one to five pairs in different accessions, and most of these sites are located at the terminal regions of chromosomes. Interestingly, up to five pairs of 45S rDNA sites were observed in C. sativus var. sativus, the species which has the lowest chromosome number, i.e., 2n = 14. Only one pair of 5S rDNA sites was detected in all accessions, except for C. heptadactylus, C. sp, and C. spp that had two pairs of 5S rDNA sites. The distributions of 5S rDNA sites showed more variation than 45S rDNA sites. The phylogenetic analysis in this study showed that 45S and 5S rDNA have contrasting evolutionary patterns. We find that 5S rDNA has a polyploidization-related tendency towards the terminal location from an interstitial location but maintains a conserved site number, whereas the 45S rDNA showed a trend of increasing site number but a relatively conserved location.

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